A5014994776- Carbohydrate Chemistry and Synthesis
- Glycosylation and Glycoproteins Research
- Enzyme Production and Characterization
- Natural product bioactivities and synthesis
- Phytochemical Studies and Bioactivities
- Pneumonia and Respiratory Infections
- Electrospun Nanofibers in Biomedical Applications
- Polysaccharides and Plant Cell Walls
- Bone Tissue Engineering Materials
- Neonatal and Maternal Infections
- Legume Nitrogen Fixing Symbiosis
- Microbial Metabolites in Food Biotechnology
- Phytochemistry and Bioactive Compounds
- Tissue Engineering and Regenerative Medicine
- Chemical Synthesis and Analysis
- Streptococcal Infections and Treatments
- Proteoglycans and glycosaminoglycans research
- Enzyme Catalysis and Immobilization
- Biochemical and Structural Characterization
- Antimicrobial Resistance in Staphylococcus
- Biochemical and Molecular Research
- Microbial Natural Products and Biosynthesis
- Infant Nutrition and Health
- Monoclonal and Polyclonal Antibodies Research
- Natural Antidiabetic Agents Studies
Shandong University
2015-2024
State Key Laboratory of Microbial Technology
2012-2021
Shandong Institute for Product Quality Inspection
2021
Qingdao Center of Resource Chemistry and New Materials
2019-2020
Zhengzhou University
2015
Wayne State University
2013
Simon Fraser University
2006
Rensselaer Polytechnic Institute
2003-2004
Research Center for Eco-Environmental Sciences
2001-2004
Chinese Academy of Sciences
2001-2004
Recent progress in the development of sortase A inhibitors as novel anti-virulence drugs for antibacterial therapy has been reviewed.
β-N-Acetylhexosaminidases have attracted interest particularly for oligosaccharide synthesis, but their use remains limited by the rarity of enzyme sources , low efficiency, and relaxed regioselectivity transglycosylation. In this work, genes 13 β-N-acetylhexosaminidases, including 5 from Bacteroides fragilis ATCC 25285, Clostridium perfringens 13124, 3 Bifidobacterium bifidum JCM 1254, were cloned heterogeneously expressed in Escherichia coli The resulting recombinant enzymes purified...
Group A streptococcus (GAS) is one of the common Gram-positive pathogenic bacteria accounting for a variety infectious diseases. Currently, there no commercial vaccine GAS. To develop efficient GAS vaccines, synthetic tri-, hexa-, and nonasaccharides conserved group carbohydrate (GAC) were conjugated with an inactive mutant streptococcal C5a peptidase (ScpA), ScpA193, to create bivalent conjugate which compared corresponding CRM197 TT conjugates. Systematic evaluations these semisynthetic...
A monophosphoryl lipid (MPLA) derivative having the 6′-OH group substituted with an NH2 was synthesized and coupled upstream terminal tetrasaccharide of mycobacterial lipoarabinomannan (LAM) via amide bond to create a novel type MPLA-based fully synthetic glycoconjugate vaccine. The same also MPLA at 1-O-position. Immunological activities two conjugates were evaluated in mice compared. Both afforded robust overall IgG antibody responses, but intraperitoneal injection elicited responses...
Two natural saponins 1 and 2, isolated from Solanum indicum L., containing 2,3-branched sugar moieties, have been efficiently synthesized. Partially protected monosaccharide disaccharide donors were used to facilitate target synthesis. Stereo factors critical in incorporating sugars on steroid aglycones. Saponin was synthesized five steps 30% overall yield, while saponin 2 obtained using six straightforward sequential reactions 31% yield. shows promising cytotoxic activity toward human...
Made visible: In situ visualization of the RBL receptor on cancer cell line surfaces was developed by using biotinylated rhamnose and chacotriose bound to quantum dots. The showed different expression patterns various lines. Also, solasodine rhamnosides were found kill cells more effectively (see graphic). finding receptor-mediated anticancer activity could help design chemotherapy agents. Detailed facts importance specialist readers are published as "Supporting Information". Such documents...
Lipoarabinomannan (LAM) is one of the major constituents Mycobacterium tuberculosis cell wall and an attractive molecular scaffold for antituberculosis drug vaccine development. In this paper, a convergent strategy was developed synthesis LAM oligosaccharides with α-1,2-linked dimannopyranose cap at nonreducing end. The highlighted by efficient coupling separately prepared end reducing oligosaccharides. Glycosylations were mainly achieved thioglycoside donors, which gave excellent yields...
A new class of glycosyl donors having unprotected 2- and 2,4-hydroxyl groups were investigated under the standard glycosylation conditions. This approach was shown to be generally effective for synthesis alkyl steroidal glycosides. natural saponin, containing 2,4-branched oligosaccharide, prepared in 35% overall yield four straightforward sequential reactions by taking advantage these partially protected donors.
The first chemical synthesis of the repeating unit serotype II group B Streptococcus capsular polysaccharide, a branched heptasaccharide α-Neu p5Ac-(2→3)-β-d-Gal p-(1→4)-β-d-GlcN pAc-(1→3)-{[β-d-Gal p-(1→6)]-β-d-Gal p}-(1→4)-β-d-Glc p-(1→3)-β-d-Glc p-(1→, was achieved by convergent [4+2+1] glycosylation, after probing different synthetic strategies and overcoming series difficulties. title compound designed to carry free amino at its downstream end enable further regioselective elaboration....
Abstract A highly convergent and efficient strategy was developed for the chemical synthesis of complex oligosaccharides Streptococcus pneumoniae type 3 capsular polysaccharides that contain multiple glucuronic acid units. Once oligoglucosides were efficiently stereoselectively assembled, designated glucose units regioselectively oxidized to in one step at final synthetic stage, which helped avoid difficult glycosylations involved acid. The target had a free amino group reducing terminus...
A convergent synthesis of GAS cell-wall oligosaccharides and their efficient conjugation with the ScpA193 carrier protein to generate glycoconjugates as potential bivalent vaccines were reported.