A5001624277- Pain Mechanisms and Treatments
- Neuroscience and Neuropharmacology Research
- Photoreceptor and optogenetics research
- Neural dynamics and brain function
- Memory and Neural Mechanisms
- Anesthesia and Neurotoxicity Research
- Pain Management and Placebo Effect
- Wound Healing and Treatments
- Musculoskeletal pain and rehabilitation
- Radiomics and Machine Learning in Medical Imaging
- Ion channel regulation and function
- Retinal Development and Disorders
- Cell Image Analysis Techniques
- Sleep and Wakefulness Research
- Electrospun Nanofibers in Biomedical Applications
- Anesthesia and Sedative Agents
- 3D Printing in Biomedical Research
- Protein purification and stability
- Immune Cell Function and Interaction
- Geophysical Methods and Applications
- Catalytic Alkyne Reactions
- biodegradable polymer synthesis and properties
- Neuroscience and Neural Engineering
- Ocular and Laser Science Research
- Botulinum Toxin and Related Neurological Disorders
Columbia University Irving Medical Center
2020-2024
Shandong University
2020-2024
Shenzhen Technology University
2024
Shenzhen University
2024
Ghent University
2024
Beijing University of Chinese Medicine
2024
Shenzhen University Health Science Center
2024
Chongqing Medical University
2023
Children's Hospital of Chongqing Medical University
2023
Nanjing University
2014-2022
The anterior cingulate cortex (ACC) is thought to be important for acute pain perception as well the development of chronic after peripheral nerve injury. Nevertheless, how ACC neurons respond sensory stimulation under states not understood. Here, we used an in vivo two-photon imaging technique monitor activity individual awake, head restrained mice. Calcium dorsal revealed robust somatic layer 5 (L5) pyramidal response noxious stimuli, and degree evoked was correlated with intensity...
Abstract Increased low frequency cortical oscillations are observed in people with neuropathic pain, but the cause of such elevated and their impact on pain development remain unclear. By imaging neuronal activity a spared nerve injury (SNI) mouse model we show that neurons dorsal root ganglia (DRG) somatosensory cortex (S1) exhibit synchronized after peripheral injury. Notably, DRG occurs within hours 1-2 days before increased oscillations. This synchrony is initiated by axotomized mediated...
Glutamate is one of the 20 common amino acids and utmost importance for chemically mediated synaptic transmission in nervous systems. To expand color palette genetically encoded indicators glutamate, we used protein engineering to develop a red intensity-based glutamate-sensing fluorescent reporter (R-iGluSnFR1). Manipulating topology R-iGluSnFR1, previously reported green indicator, led development noncircularly permutated (ncp) variants. R- Rncp-iGluSnFR1 display glutamate affinities 11 μM...
Significance Neuropathic pain affects nearly 10% of the US population, and yet current treatments with small-molecule drugs fail to adequately alleviate nociception often produce serious side effects. High-voltage-activated calcium channels (HVACCs) are membrane proteins that necessary for synaptic transmission in sensory neurons, inhibiting these is a proven method achieve analgesia. In this work, we used subcutaneous injection express genetically encoded molecule blocks HVACCs neurons...
The increasing micro- and nanoplastic (MNP) pollution poses significant risks to human animal health, yet the mechanisms of their accumulation effects on absorptive tissues such as gastrointestinal tract remain poorly understood. Addressing these knowledge gaps requires tractable models coupled dynamic live cell imaging methods, enable multi-parameter analysis at single resolution. Here we report a new method combining adult stem cell-derived small intestinal organoid cultures with...
Antineoplastic agents, including vincristine, often induce neuropathic pain and magnesium deficiency clinically, but the causal link between them has not been determined. No drug is available for treating this form of pain.Injection vincristine (0.1 mg · kg day, intraperitoneally, 10 days) was used to nociceptive sensitization, which accessed with von Frey hairs plantar tester in adult male Sprague-Dawley rats. Magnesium-L- threonate administered through drinking water (604 day)....
The TRPM7 chanzyme contributes to several biological and pathological processes in different tissues. However, its role the CNS under physiological conditions remains unclear. Here, we show that knockdown hippocampal neurons reduces structural synapse density. density is rescued by α-kinase domain C terminus but not ion channel region of or increasing extracellular concentrations Mg2+ Zn2+. Early postnatal conditional knockout mice impairs learning memory plasticity. hippocampus adult rats...
Abstract Synapses at dendritic branches exhibit specific properties for information processing. However, how the synapses are orchestrated to dynamically modify their properties, thus optimizing processing, remains elusive. Here, we observed hippocampal diverse configurations of synaptic connectivity, two extremes which characterized by low transmission efficiency, high plasticity and coding capacity, or inversely. The former favors encoding, pertinent learning, while latter prefers storage,...
While dysfunction of the medial prefrontal cortex (mPFC) has been implicated in chronic pain, underlying neural circuits and contribution specific cellular populations remain unclear. Using vivo Ca2+ imaging, we report that both male female mice, peripheral nerve injury-induced neuropathic pain causes a marked reduction vasoactive intestinal polypeptide (VIP)-expressing interneuron activity prelimbic area mPFC, which contributes to decreased cortical outputs. Moreover, glutamatergic...
Aging is accompanied by a progressive decrease in learning and memory function. Synaptic loss, one of the hallmarks normal aging, likely plays an important role age-related cognitive decline. But little known about impact advanced age on synaptic plasticity neuronal function vivo . In this study, we examined structural dynamics postsynaptic dendritic spines as well calcium activity layer 5 pyramidal neurons cerebral cortex young old mice. Using transcranial two-photon microscopy, found that...
The density of functional synapses is an important parameter in determining the efficacy synaptic transmission. However, how presynaptic terminal regulated under natural physiological conditions still poorly understood. We studied factors controlling terminals at single dendritic branches hippocampal neurons and found that elevation intracellular Mg2+ concentration was effective increasing terminals. Interestingly, upregulation not due to synaptogenesis, but conversion a considerable...
Multicellular tumor spheroids are a popular 3D tissue microaggregate model for reproducing microenvironment, testing and optimizing drug therapies using bio- nanosensors in context. Their ease of production, predictable size, growth, observed nutrient metabolite gradients important to recapitulate the niche-like cell microenvironment. However, spheroid heterogeneity variability their production methods can influence overall metabolism, viability, response. This makes it difficult choose most...
Abstract Anesthesia can influence cerebral blood flow by altering vessel diameter. Using in vivo two-photon imaging, we examined the effects of volatile anesthetics, sevoflurane and isoflurane, on diameter young adult mice. Our results show that these anesthetics induce robust dilation cortical arterioles arteriole-proximate capillaries mice, with milder juveniles no infants. This anesthesia-induced vasodilation correlates decreased cytosolic Ca 2+ levels NG2 + vascular mural cells....
This study presents to develop a modified acellular porcine corneal matrix (MAPCM) maintain high transparency, stability and biocompatibility as rabbit deep cornea replacement using 1-ethyl-3–(3-dimethylaminopropyl)-carbodiimide crosslinking mild decellularization technique. Scaffolds are translucent remain higher amount of glycosaminoglycans after than (APCM). Enzymatic degradation kinetics mechanical properties scaffolds regulated by -crosslinking density. The porous structure...