A5053675071- Neuroscience and Neuropharmacology Research
- Neural dynamics and brain function
- Neurogenesis and neuroplasticity mechanisms
- Receptor Mechanisms and Signaling
- Neuroinflammation and Neurodegeneration Mechanisms
- Pluripotent Stem Cells Research
- Anesthesia and Neurotoxicity Research
- Neuroscience and Neural Engineering
- 3D Printing in Biomedical Research
- Connexins and lens biology
- CRISPR and Genetic Engineering
- Retinal Development and Disorders
- Single-cell and spatial transcriptomics
- Photoreceptor and optogenetics research
- RNA modifications and cancer
- Ferroptosis and cancer prognosis
- Genomics and Rare Diseases
- Functional Brain Connectivity Studies
- Genomics and Phylogenetic Studies
- Glioma Diagnosis and Treatment
- Genetics and Neurodevelopmental Disorders
- Cancer Immunotherapy and Biomarkers
- Ion channel regulation and function
- Nuclear Receptors and Signaling
- Bone health and treatments
Fudan University
2016-2025
State Key Laboratory of Medical Neurobiology
2015-2025
Tongji University
2003-2024
ShanghaiTech University
2024
University of California, Los Angeles
2024
Harbin Medical University
2021-2023
First Affiliated Hospital of Harbin Medical University
2021-2023
Institute of Neurobiology
2021
Chinese Institute for Brain Research
2015-2020
Huashan Hospital
2017-2018
Astrocytes, due to the proximity neuronal lineage and capability proliferate, are ideal starting cells regenerate neurons. Human fetal astrocytes have been successfully converted into by small molecules, which offered a broader range of further applications than transcription factor-mediated reprogramming. Here we report that human adult could also be different set molecules. These induced exhibited typical morphologies, expressed markers, displayed electrophysiological properties....
Abstract The apolipoprotein E4 ( APOE4 ) genotype is one of the strongest genetic risk factors for Alzheimer’s disease (AD), and generally believed to cause widespread pathological alterations in various types brain cells. Here, we developed a novel engineering method creating chimeric human cerebral organoids (chCOs) assess differential roles neurons astrocytes. First, astrogenic NFIB SOX9 were introduced into induced pluripotent stem cells (iPSCs) accelerate induction Then above iPSCs...
Eye opening, a natural and timed event during animal development, influences cortical circuit assembly maturation; yet, little is known about its precise effect on inhibitory synaptic connections. Here, we show that coinciding with eye the strength of unitary postsynaptic currents (uIPSCs) from somatostatin-expressing interneurons (Sst-INs) to nearby excitatory neurons, but not interneurons, sharply decreases in layer 2/3 mouse visual cortex. In contrast, uIPSCs fast-spiking (FS-INs) neurons...
Abstract In the neocortex, gap junctions are expressed at very early developmental stages, and they involved in many processes such as neurogenesis, neuronal migration synapse formation. Connexin43 (Cx43), a junction protein, has been found to be abundantly radial glial cells, excitatory neurons astrocytes. Although accumulating evidence suggests that Cx43-mediated gap-junctional coupling between astrocytes plays an important role central nervous system, function of Cx43 remains elusive....
Layer 1 of the neocortex harbors a unique group neurons that play crucial roles in synaptic integration and information processing. Although extensive studies have characterized properties layer mature neocortex, it remains unclear how these progressively acquire their distinct morphological, neurochemical, physiological traits. In this study, we systematically examined dynamic development Cajal-Retzius cells γ-aminobutyric acid (GABA)-ergic interneurons during first 2 postnatal weeks....
The mammalian brain is heterogeneous, containing billions of neurons and trillions synapses forming various neural circuitries, through which sense, movement, thought, emotion arise. cellular heterogeneity the has made it difficult to study molecular logic circuitry wiring, pruning, activation, plasticity, until recently, transcriptome analyses with single cell resolution makes decoding gene regulatory networks underlying aforementioned properties possible. Here we report success in...
Abstract Clear cell renal carcinoma (ccRCC) is the most common subtype of kidney cancer and associated with poor prognosis. The histone H3 lysine 36 methyltransferase SET-domain-containing 2 (SETD2) has been reported to be expressed at low levels frequently mutated in ccRCC. Ferroptosis, a form death distinct from apoptosis necrosis, recent years cancer. However, relationship between SETD2 ferroptosis not clear. Here, we demonstrated that was ccRCC Moreover, found knockdown increased lipid...
Tooth regeneration is considered to be an optimistic approach replace current treatments for tooth loss. It important determine the most suitable seed cells regeneration. Recently, human umbilical cord mesenchymal stem (hUCMSCs) have been regarded as a promising candidate tissue However, it has not reported whether hUCMSCs can employed in Here, we report that induced into odontoblast-like vitro and vivo. Induced expressed dentin-related proteins including dentin sialoprotein (DSP) matrix...
The TRPM7 chanzyme contributes to several biological and pathological processes in different tissues. However, its role the CNS under physiological conditions remains unclear. Here, we show that knockdown hippocampal neurons reduces structural synapse density. density is rescued by α-kinase domain C terminus but not ion channel region of or increasing extracellular concentrations Mg2+ Zn2+. Early postnatal conditional knockout mice impairs learning memory plasticity. hippocampus adult rats...
RACK7 recognizes H3.3G34R to suppress expression of MHC II complex components and their delivery pathway in pediatric GBM.
Abstract The coexistence of electrical and chemical synapses among interneurons is essential for interneuron function in the neocortex. However, it remains largely unclear whether coupling between influences synapse formation microcircuit assembly during development. Here, we show that GABAergic connections robustly develop neocortical layer 1 over a similar time course. Electrical promotes action potential generation synchronous firing interneurons. Furthermore, electrically coupled exhibit...
Glioblastoma (GBM) is the most common and aggressive malignant tumor in adult brain. Even with current standard therapy including surgical resection followed by postoperative radiotherapy chemotherapy temozolomide (Temo), GBM patients still have a poor median survival. Reprogramming of cells into non-malignant might be promising therapeutic strategy for tumors, GBM. Based on previous studies using small molecules to reprogram astrocytes neuronal cells, here we further identified FTT cocktail...
Natriuretic peptides (NPs) may work as neuromodulators through their associated receptors [NP (NPRs)]. By immunocytochemistry, we showed that NPR-A and NPR-B were expressed abundantly on both ON-type OFF-type bipolar cells (BCs) in rat retina, including the dendrites, somata, axon terminals. Whole-cell recordings made from isolated BCs further brain natriuretic peptide (BNP) suppressed GABAA receptor-, but not GABAC mediated currents of BCs, which was blocked by antagonist anantin. The NPR-C...
Abstract Inwardly rectifying potassium channels (Kir channels) are important for neuronal signalling and membrane excitability. In the present work we characterized, first time, Kir in rat retinal ganglion cells (RGCs), output neurons retina, using immunocytochemical patch‐clamp techniques. Various subunits of (Kir1.1, 2.1, 2.3, 3.1, 3.2 3.3) were expressed RGCs, but with distinct subcellular localization. Kir1.1 was mainly axons RGCs. Kir2.1 Kir2.3 both somata Whereas staining Kir3.1...
GABAergic medium-sized spiny neurons (MSNs) in the nucleus accumbens (NAc) differentially express D1 and D2 dopamine receptors. Both D2- D1-MSNs NAc form projections into ventral pallidum, whereas only directly project midbrain neurons. They are critical rewarding aversive learning, understanding function of these efferents alteration their targeted brain regions responding to a reward-associated context is important. In this study, we activated mice expressing channelrhodopsin-2 under...
Electrical coupling between excitatory neurons in the neocortex is developmentally regulated. It initially prominent but eliminated at later developmental stages when chemical synapses emerge. However, it remains largely unclear whether early electrical networks broadly contribute to neocortical circuit formation and animal behavior. Here, we report that neonatal critical for proper neuronal development, synapse formation, Conditional deletion of Connexin 26 (CX26) superficial layer mouse...