A5018860616- Hearing, Cochlea, Tinnitus, Genetics
- Cellular Mechanics and Interactions
- Cancer-related molecular mechanisms research
- Hearing Loss and Rehabilitation
- Vestibular and auditory disorders
- Cancer Treatment and Pharmacology
- 3D Printing in Biomedical Research
- Climate variability and models
- Ear Surgery and Otitis Media
- Arctic and Antarctic ice dynamics
- Developmental Biology and Gene Regulation
- Neuroinflammation and Neurodegeneration Mechanisms
- Silk-based biomaterials and applications
- Pain Mechanisms and Treatments
- Nerve injury and regeneration
- Biochemical Analysis and Sensing Techniques
- Bone Tissue Engineering Materials
- Electrospun Nanofibers in Biomedical Applications
- Antimicrobial Peptides and Activities
- Epigenetics and DNA Methylation
- Antibiotic Resistance in Bacteria
- RNA modifications and cancer
- Cancer, Hypoxia, and Metabolism
- Ear and Head Tumors
- Noise Effects and Management
Eye & ENT Hospital of Fudan University
2016-2025
State Key Laboratory of Medical Neurobiology
2016-2025
Nanjing Medical University
2025
University of Washington
2025
Yueyang Hospital
2024
Shanghai University of Traditional Chinese Medicine
2024
Huazhong University of Science and Technology
2006-2024
Fudan University
2013-2024
National Health and Family Planning Commission
2016-2024
NOAA Global Systems Laboratory
2022-2024
The role of cytosolic calcium oscillation has long been recognized in the regulation cellular and molecular interactions. Information embedded can provide cues for cell behavior such as differentiation. Although dynamics are versatile likely to depend on type, human mesenchymal stem cells (hMSCs) its differentiation yet be fully elucidated. In present study we characterized profiles hMSCs before after subjecting osteoinductive factors. Our findings indicate that spikes decreased rapidly with...
Significance Notch signaling is known as a fundamental pathway that regulates the cell-fate determination in inner ear. In present study, we show also acts negative regulator inhibits proliferation of Lgr5 + progenitors and maintains homeostasis cochlear sensory epithelium on cell numbers. More importantly, to our knowledge provide first piece evidence illustrating interaction between Wnt postal mouse cochlea: inhibition activates canonical progenitor cells, which leads mitotic generation...
Abstract Recent studies have reported the role of Wnt/ β -catenin signaling in hair cell (HC) development, regeneration, and differentiation mouse cochlea; however, HC protection remains unknown. In this study, we took advantage transgenic mice to specifically knockout or overactivate canonical Wnt mediator HCs, which allowed us investigate protecting HCs against neomycin-induced damage. We first showed that loss made them more vulnerable injury, while constitutive activation reduced both...
Abstract Many cancer drugs are toxic to cells by activating apoptotic pathways. Previous studies have shown that mitochondria key roles in apoptosis mammalian cells, but the role of mitochondrial DNA (mtDNA) copy number variation pathogenesis tumor cell remains largely unknown. We used HEp-2, HNE2, and A549 lines explore relationship between mtDNA apoptosis. first induced three one normal adult human skin fibroblast line (HSF) with cisplatin (DDP) or doxorubicin (DOX) treatment found...
Cochlear implantation has become the most effective treatment method for patients with profound and total hearing loss. However, its therapeutic efficacy is dependent on number normal physiological function of cochlear implant-targeted spiral ganglion neurons (SGNs). Electrical stimulation can be used as an cue to regulate morphology excitatory cells. Therefore, it important develop efficient implant electroacoustic (EAS) system study behavior SGNs. In this work, we present electrical...
Abstract Antimicrobial resistance (AMR) is a pressing issue in China, with antibiotic therapy becoming less effective against bacterial infections. To address this challenge, the China Surveillance Network (CHINET) was established 2005 to monitor antimicrobial country. This study analyzed CHINET data from teaching hospitals and evaluated trends of AMR 2018 2022. A range 163,636 301,917 isolates obtained per year, majority being Gram-negative bacilli (69.0% 71.8%). The proportion important...
Fractalkine, a chemokine binding to only one known receptor CX3CR1, has recently been proposed be neuron-to-glia signal in the spinal cord leading microglial activation and glially dependent pain facilitation. The previous studies explored that blockade of endogenous fractalkine, using anti-CX3CR1 neutralizing antibody, dose-dependently attenuated neuropathic pain. present study examined role fractalkine inflammatory Intra-articular injection complete Freund's adjuvant (CFA)-induced rat...
Abstract This work sought to determine the crosstalk between Notch and Wnt signaling pathways in regulating supporting cell (SC) proliferation hair (HC) regeneration mouse utricles. We cultured postnatal day (P)3 P60 utricles, damaged HCs with gentamicin, treated utricles γ-secretase inhibitor DAPT inhibit pathway agonist QS11 active pathway. also used Sox2-CreER, Notch1-flox (exon 1), Catnb-flox 3) transgenic mice knock out activate Sox2+ SCs. inhibition alone increased SC HC number both...
Hair cell (HC) loss is the main cause of permanent hearing in mammals. Previous studies have reported that neonatal mice cochleae, Wnt activation promotes supporting (SC) proliferation and Notch inhibition trans-differentiation SCs into HCs. However, alone fails to regenerate significant amounts new HCs, regenerates HCs at cost exhausting SC population, which leads death newly regenerated Mitotic HC regeneration might preserve number while regenerating could be a better approach for...
Abstract OTOF mutations are the principal causes of auditory neuropathy. There reports on Otof ‐related gene therapy in mice, but there is no preclinical research drug evaluations. Here, Anc80L65 and mouse hair cell‐specific Myo15 promoter (mMyo15) used to selectively effectively deliver human cells mice nonhuman primates evaluate efficacy safety drugs. A new dual‐AAV ‐OTOF‐ hybrid strategy transfer full‐length generated, which can stably restore hearing adult p.Q939*/Q939* with profound...
The genetic heterogeneity of non-syndromic hearing loss (NSHL) has hampered the identification its pathogenic mutations. Several recent studies applied targeted genome enrichment (TGE) and massively parallel sequencing (MPS) to simultaneously screen a large set known (HL) genes. However, most these were focused on familial cases. To evaluate effectiveness TGE MPS screening sporadic NSHL patients, we recruited 63 unrelated probands, who had various levels HL excluded for mutations in GJB2,...
Aminoglycoside-induced cochlear ototoxicity causes hair cell (HC) loss and results in hearing impairment patients. Previous studies have developed the concept of an ototoxicity-sensitive period during which cochleae young mice are more vulnerable to auditory trauma than adults. Here, we compared neomycin-induced at following four developmental ages mice: postnatal day (P)1-P7, P8-P14, P15-P21, P60-P66. We found that when neomycin was administered between P8 P14, brainstem response threshold...
Hair cell (HC) loss is the major cause of permanent sensorineural hearing in mammals. Unlike lower vertebrates, mammalian cochlear HCs cannot regenerate spontaneously after damage, although vestibular system does maintain limited HC regeneration capacity. Thus from damaged sensory epithelium has been one main areas research field restoration. Hedgehog signaling plays important roles during embryonic development inner ear, and it involved progenitor proliferation differentiation as well fate...