A5100345161- Lipid metabolism and biosynthesis
- Plant Molecular Biology Research
- Cancer-related molecular mechanisms research
- Photosynthetic Processes and Mechanisms
- Plant Virus Research Studies
- Genomics and Phylogenetic Studies
- Plant Stress Responses and Tolerance
- RNA modifications and cancer
- Genetic Mapping and Diversity in Plants and Animals
- GABA and Rice Research
- Food composition and properties
- CRISPR and Genetic Engineering
- Research in Cotton Cultivation
- Plant nutrient uptake and metabolism
- Microbial Metabolic Engineering and Bioproduction
- Plant Disease Resistance and Genetics
- Plant tissue culture and regeneration
- Rice Cultivation and Yield Improvement
- Biofuel production and bioconversion
- Plant and Fungal Interactions Research
- Plant biochemistry and biosynthesis
- Plant responses to water stress
- Plant Gene Expression Analysis
- RNA and protein synthesis mechanisms
- Insect Resistance and Genetics
Agriculture and Food
2016-2025
Commonwealth Scientific and Industrial Research Organisation
2016-2025
University of Chinese Academy of Sciences
2011-2025
Yangzhou University
2012-2025
Wuhan Polytechnic University
2024-2025
Huazhong University of Science and Technology
2025
Hunan Agricultural University
2009-2025
Chengdu University of Technology
2025
Nanjing Agricultural University
2001-2025
Shandong University
2020-2025
Post-transcriptional silencing of plant genes using anti-sense or co-suppression constructs usually results in only a modest proportion silenced individuals. Recent work has demonstrated the potential for encoding self-complementary 'hairpin' RNA (hpRNA) to efficiently silence genes. In this study we examine design rules efficient gene silencing, terms both independent transgenic plants showing and degree silencing. Using hpRNA containing sense/anti-sense arms ranging from 98 853 nt gave...
Spinal muscular atrophy (SMA) is an often fatal neuromuscular disease that has been directly linked to the protein product of Survival Motor Neurons (SMN) gene. The SMN tightly associated with a novel protein, SIP1, and together they form complex several spliceosomal snRNP proteins. Here we show SMN–SIP1 snRNAs U1 U5 in cytoplasm Xenopus oocytes. Antibodies directed against strongly interfere cytoplasmic assembly common (Sm) proteins import into nucleus. Thus, involved biogenesis snRNPs....
Summary High biomass crops have recently attracted significant attention as an alternative platform for the renewable production of high energy storage lipids such triacylglycerol ( TAG ). While typically accumulates in seeds compounds fuelling subsequent germination, levels vegetative tissues are generally low. Here, we report accumulation more than 15% (17.7% total lipids) by dry weight N icotiana tabacum (tobacco) leaves co‐expression three genes involved different aspects without...
Abstract As a resource allocation strategy, plant growth and defense responses are generally mutually antagonistic. Brassinosteroid (BR) regulates many aspects of development stress responses, however, genetic evidence its integrated effects on tolerance is lacking. We overexpressed the Arabidopsis BR biosynthetic gene AtDWF4 in oilseed Brassica napus scored response phenotypes. The transgenic B. plants, comparison to wild type, displayed increased seed yield leading overall oil content per...
Summary Plant laccase ( LAC ) enzymes belong to the blue copper oxidase family and polymerize monolignols into lignin. Recent studies have established involvement of micro RNA s in this process; however, physiological functions regulation plant laccases remain poorly understood. Here, we show that a gene, 4 , regulated by miR397b, controls both lignin biosynthesis seed yield Arabidopsis. In transgenic plants, overexpression miR397b OX miR397b) reduced deposition. The secondary wall thickness...
Background Omega-3 long-chain (≥C20) polyunsaturated fatty acids (ω3 LC-PUFA) such as eicosapentaenoic acid (EPA) and docosapentaenoic (DHA) are critical for human health development. Numerous studies have indicated that deficiencies in these can increase the risk or severity of cardiovascular, inflammatory other diseases disorders. EPA DHA predominantly sourced from marine fish although primary producers microalgae. Much work has been done to engineer a sustainable land-based source reduce...
DNA quantification is important for biomedical research, but the routinely used techniques rely on nucleic acid amplification which have inherent issues like cross-contamination risk and bias. Here, we report a CRISPR-Cas12a-based molecular diagnostic technique amplification-free absolute of at single-molecule level. To achieve this, first screened out optimal reaction parameters high-efficient Cas12a assay, yielding over 50-fold improvement in sensitivity compared with reported assays. We...
We have genetically modified the fatty acid composition of cottonseed oil using recently developed technique hairpin RNA-mediated gene silencing to down-regulate seed expression two key desaturase genes, ghSAD-1-encoding stearoyl-acyl-carrier protein Delta 9-desaturase and ghFAD2-1-encoding oleoyl-phosphatidylcholine omega 6-desaturase. Hairpin RNA-encoding constructs (HP) targeted against either ghSAD-1 or ghFAD2-1 were transformed into cotton (Gossypium hirsutum cv Coker 315). The...
Understanding the fundamental nature of a molecular process or biological pathway is often catalyst for development new technologies in biology. Indeed, studies from late 1990s to early 2000s have uncovered multiple overlapping but functionally distinct RNA silencing pathways plants, including posttranscriptional microRNA and small interfering transcriptional RNA-directed DNA methylation pathway. These findings turn been exploited developing artificial such as hairpin RNA, microRNA,...
Background Omega-3 long-chain (≥C20) polyunsaturated fatty acids (ω3 LC-PUFA) have critical roles in human health and development with studies indicating that deficiencies these can increase the risk or severity of cardiovascular inflammatory diseases particular. These are predominantly sourced from fish algal oils, but it is widely recognised there an urgent need for alternative sustainable source EPA DHA. Since earliest demonstrations ω3 LC-PUFA engineering has been good progress C20 seed...
Astrocytes, due to the proximity neuronal lineage and capability proliferate, are ideal starting cells regenerate neurons. Human fetal astrocytes have been successfully converted into by small molecules, which offered a broader range of further applications than transcription factor-mediated reprogramming. Here we report that human adult could also be different set molecules. These induced exhibited typical morphologies, expressed markers, displayed electrophysiological properties....
Rationale: Recent advances have improved our ability to generate cardiomyocytes from human induced pluripotent stem cells (hiPSCs) and embryonic (hESCs). However, understanding of the transcriptional regulatory networks underlying early stages (ie, mesoderm cardiac mesoderm) cardiomyocyte differentiation remains limited. Objective: To characterize transcriptome chromatin accessibility during hiPSCs hESCs. Methods Results: We profiled temporal changes in at genome-wide levels derived 2 hiPSC...
Synthesis and accumulation of plant oils in the entire vegetative biomass offers potential to deliver yields surpassing those oilseed crops. However, current levels still fall well short typically found oilseeds. Here we show how transcriptome biochemical analyses pointed a futile cycle previously established Nicotiana tabacum line, accumulating up 15% (dry weight) storage lipid triacylglycerol leaf tissue. To overcome this metabolic bottleneck, either silenced SDP1 lipase or overexpressed...
Plant cryptochromes undergo blue light-dependent phosphorylation to regulate their activity and abundance, but the protein kinases that phosphorylate plant have remained unclear. Here we show photoexcited Arabidopsis cryptochrome 2 (CRY2) is phosphorylated in vivo on as many 24 different residues, including 7 major phosphoserines. We demonstrate four closely related Photoregulatory Protein Kinases (previously referred MUT9-like kinases) interact with CRY2. Analyses of ppk123 ppk124 triple...