A5020862291- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- Microfluidic and Capillary Electrophoresis Applications
- CRISPR and Genetic Engineering
- SARS-CoV-2 detection and testing
- Nanoplatforms for cancer theranostics
- Innovative Microfluidic and Catalytic Techniques Innovation
- RNA Interference and Gene Delivery
- Antimicrobial Peptides and Activities
- 3D Printing in Biomedical Research
- Photodynamic Therapy Research Studies
- Viral Infections and Immunology Research
- Microfluidic and Bio-sensing Technologies
- Micro and Nano Robotics
- Marine and coastal ecosystems
- Herpesvirus Infections and Treatments
- Poxvirus research and outbreaks
- MicroRNA in disease regulation
- RNA and protein synthesis mechanisms
- Parkinson's Disease Mechanisms and Treatments
- Aquatic Ecosystems and Phytoplankton Dynamics
- Mosquito-borne diseases and control
- Viral gastroenteritis research and epidemiology
- Electrowetting and Microfluidic Technologies
- Optical Imaging and Spectroscopy Techniques
Southern Medical University
2021-2025
University of Chinese Academy of Sciences
2023
Aerospace Information Research Institute
2023
Guangzhou First People's Hospital
2016-2021
Guangzhou Medical University
2016-2021
Key Laboratory of Guangdong Province
2016-2021
South China University of Technology
2017-2021
South China Normal University
2013-2017
First Affiliated Hospital of Guangzhou Medical University
2016
Existing methods for RNA diagnostics, such as reverse transcription PCR (RT-PCR), mainly rely on nucleic acid amplification (NAA) and RT processes, which are known to introduce substantial issues, including bias, cross-contamination, sample loss. To address these problems, we a confinement effect-inspired Cas13a assay single-molecule eliminating the need NAA RT. This involves confining RNA-triggered catalysis system in cell-like-sized reactors enhance local concentrations of target reporter...
DNA quantification is important for biomedical research, but the routinely used techniques rely on nucleic acid amplification which have inherent issues like cross-contamination risk and bias. Here, we report a CRISPR-Cas12a-based molecular diagnostic technique amplification-free absolute of at single-molecule level. To achieve this, first screened out optimal reaction parameters high-efficient Cas12a assay, yielding over 50-fold improvement in sensitivity compared with reported assays. We...
The recent prevalence of monkeypox has led to the declaration a Public Health Emergency International Concern. Monkeypox lesions are typically ulcers or pustules (containing high titers replication-competent virus) in skin and mucous membranes, which allow virus transmit predominantly through intimate contact. Currently, effective clinical treatments for lacking, strategies blocking transmission fraught with drawbacks. Herein, this work constructs biomimetic nanotemplate (termed TBD@M NPs)...
Abstract Tuberculosis, a fatal infectious disease caused by Mycobacterium tuberculosis ( M.tb ), is difficult to treat with antibiotics due drug resistance and short half‐life. Phototherapy represents promising alternative in combating . Exploring an intelligent material allowing effective treatment definitely appealing, yet significantly challenging task. Herein, all‐in‐one biomimetic therapeutic nanoparticle featured aggregation‐induced second near‐infrared emission, granuloma‐targeting,...
Spinal cord injury (SCI), following explosive oxidative stress, causes an abrupt and irreversible pathological deterioration of the central nervous system. Thus, preventing secondary injuries caused by reactive oxygen species (ROS), as well monitoring assessing recovery from SCI are critical for emergency treatment SCI. Herein, strategy is developed based on selenium (Se) matrix antioxidant system to effectively inhibit stress-induced damage simultaneously real-time evaluate severity using a...
Tuberculosis (TB) is a virulent form of an infectious disease that causes global burden due to its high infectivity and fatality rate, especially the irrepressible threats latent infection. Constructing efficient strategy for prevention control TB great significance. Fortunately, we found granulomas are endowed with higher reducibility levels possibly caused by internal inflammation relatively enclosed microenvironment. Therefore, developed first targeted glutathione- (GSH-) responsive...
Electrochemical (EC) sensors have been widely developed for DNA detection, but they are seldom used in a simple, economic, and efficient manner. In this work, the first time, EC cloth-based (ECDSs) as new class of sensors, without need cumbersome chip fabrication high-cost peripheral facilities. Carbon ink- solid wax-based screen printing were to produce ultracheap sensing devices (the cost one sensor is estimated be $0.045). Also, CdTe QDs/MWCNTs nanocomposite (CdTe-MWCNTs) was applied...
Abstract Point‐of‐care (POC) pathogen detection is highly desirable in diverse fields such as infectious disease diagnosis, food safety testing, and environmental monitoring. Herein, the study seeks to address this critical need by developing an automated microfluidic photothermal quantitative polymerase chain reaction (AMP‐qPCR) system a greatly simplified format. A key element of AMP‐qPCR architecture that combines design clockwork‐like, magnetically‐driven multi‐chamber cartridge with use...
Rapid and accurate screening of pathogens is crucial for disease detection. Here, a modular microfluidic sensor has been constructed RNA detection, with integrated nucleic acid extraction, clustered regularly interspaced short palindromic repeats (CRISPR)/Cas13a reaction, electrochemiluminescence (ECL) The consists processing detection modules. module used distribution, the CRISPR/Cas reaction. Specifically, immiscible filtration assisted by surface tension employed significantly reducing...
Highlights•Droplet-based assay for precise multiplexed quantification of EV membrane proteins•α-Synuclein is associated with the on surface L1CAM+ neuronal EVs•α-Synuclein association increases under pathological conditions•EV α-synuclein an improved biomarker early Parkinson's diagnosticsSummaryAccurate diagnosis disease requires platforms suitable detecting minute amounts neuronally derived biomarkers in massive protein excess easily accessible biofluids such as blood. Here, we describe...
An ultra-compact, battery-powered and easy-to-use microfluidic platform is proposed for rapid, sensitive, multiplex real-time quantification point-of-care genetic analysis.
A microfluidic chip was developed for one-step identification and antimicrobial susceptibility testing (AST) of multiple uropathogens. The polydimethylsiloxane (PDMS) microchip used had features cell culture chamber arrays connected through a sample introduction channel. At the bottom each chamber, paper substrate preloaded with chromogenic media agents embedded. By integrating hydrophobic membrane valve on microchip, urine can be equally distributed into confined in individual chambers. AST...
Spinal Cord Injuries In article number 2207888, Xianjin Du, Jian Zhang, Hongsheng Lin, Yuhui Liao, and co-workers design an emergency treatment photoacoustic severity assessment (ETPSA) strategy for spinal cord injury (SCI) based on the selenium (Se) matrix antioxidant system Se@BDP-DOH nanoparticles, which can real-time evaluate of SCI using signals simultaneously effectively inhibit oxidative stress-induced damage in neuron cells to reconstruct redox microenvironment, accompanying...
Rapid and sensitive RNA detection is of great value in diverse areas, ranging from biomedical research to clinical diagnostics. Existing methods for often rely on reverse transcription (RT) DNA amplification or involve a time-consuming procedure poor sensitivity. Herein, we proposed CRISPR/Cas12a-enabled amplification-free assay rapid, specific, This assay, which termed T7/G4-CRISPR, involved the use T7-powered nucleic acid circuit convert single target into numerous activators via...