A5004968028- Neuroscience and Neuropharmacology Research
- Pain Mechanisms and Treatments
- Ion channel regulation and function
- Cardiac electrophysiology and arrhythmias
- Neural dynamics and brain function
- Photoreceptor and optogenetics research
- Receptor Mechanisms and Signaling
- Retinal Development and Disorders
- Neuroscience and Neural Engineering
- Advanced Fluorescence Microscopy Techniques
- Plant-based Medicinal Research
- Zebrafish Biomedical Research Applications
- Neurobiology and Insect Physiology Research
- Amino Acid Enzymes and Metabolism
- Epigenetics and DNA Methylation
- Cell Image Analysis Techniques
- Neurological diseases and metabolism
- Animal testing and alternatives
- Single-cell and spatial transcriptomics
- Cardiovascular Effects of Exercise
- Mitochondrial Function and Pathology
- Anesthesia and Sedative Agents
- Prion Diseases and Protein Misfolding
- Nerve injury and regeneration
- Eicosanoids and Hypertension Pharmacology
McGill University
2014-2024
Canadian Nuclear Laboratories
2020-2021
SNC-Lavalin (Canada)
2021
Université Laval
2019
Montreal Neurological Institute and Hospital
2012-2017
Therapeutics Clinical Research
2015
Stornoway Historical Society
2013
Université de Bordeaux
2011-2013
Inserm
2007-2013
Université Clermont Auvergne
2007-2011
Dynamic mechanical allodynia is a widespread and intractable symptom of neuropathic pain for which there lack effective therapy. During tactile allodynia, activation the sensory fibers normally detect touch elicits pain. Here we provide new behavioral investigation into dynamic component that developed in rats after segmental removal glycine inhibition. Using vivo electrophysiological recordings, show this condition innocuous stimuli could activate superficial dorsal horn nociceptive...
Dynamic mechanical allodynia is a widespread and intractable symptom of neuropathic pain for which there lack effective therapy. We recently provided novel perspective on the mechanisms this by showing that simple switch in trigeminal glycine synaptic inhibition can turn touch into unmasking innocuous input to superficial dorsal horn nociceptive specific neurons through local excitatory, NMDA-dependent neural circuit involving expressing gamma isoform protein kinase C. Here, we further...
Parvalbumin-expressing interneurons (PVINs) play a crucial role within the dorsal horn of spinal cord by preventing touch inputs from activating pain circuits. In both male and female mice, nerve injury decreases PVINs' output via mechanisms that are not fully understood. this study, we show PVINs nerve-injured mice change their firing pattern tonic to adaptive. To examine ionic responsible for decreased output, used reparametrized Hodgkin-Huxley type model PVINs, which predicted (1)...
Glycine inhibitory dysfunction provides a useful experimental model for studying the mechanism of dynamic mechanical allodynia, widespread and intractable symptom neuropathic pain. In this model, allodynia expression relies on N-methyl-d-aspartate receptors (NMDARs), it has been shown that astrocytes can regulate their activation through release NMDAR coagonist d-serine. Recent studies also suggest potentially contribute to However, involvement in remains unknown. Here, we show after...
Summary Bone cancer pain is not always correlated with spinal overexpression of reactive glia markers, in contrast to neuropathic pain. a common and disruptive symptom patients. In animal models, massive astrogliosis the dorsal horn cord has been reported. Because astrocytes may behave as driving partners for pathological pain, we investigated temporal development behavior rat bone model induced by injecting MRMT-1 mammary gland carcinoma cells into tibia. Along lesions, gradual mechanical...
The NMDAR is thought to play a key role in the refinement of connectivity developing neural circuits. Pharmacological blockade or genetic loss-of-function manipulations that prevent function during development result disorganization topographic axonal projections. However, because NMDARs contribute overall glutamatergic neurotransmission, such experiments fail adequately distinguish between roles played by and activity general. gliotransmitter d-serine coagonist required for channel opening,...
Type 1 cannabinoid receptors (CB1Rs) are widely expressed in the vertebrate retina, but role of endocannabinoids vision is not fully understood. Here, we identified a novel mechanism underlying CB1R-mediated increase retinal ganglion cell (RGC) intrinsic excitability acting through AMPK-dependent inhibition NKCC1 activity. Clomeleon imaging and patch clamp recordings revealed that downstream CB1R activation reduces intracellular Cl− levels RGCs, hyperpolarizing resting membrane potential. We...
The developing retinotectal system of the Xenopus laevis tadpole is a model choice for studying visual experience-dependent circuit maturation in intact animal. neurotransmitter gamma-aminobutyric acid (GABA) has been shown to play critical role formation sensory circuits this preparation, however comprehensive neuroanatomical study GABAergic cell distribution not conducted. We report detailed description spatial expression GABA immunoreactivity brain at two key developmental stages: stage...
Activity-dependent strengthening of central synapses is a key factor driving neuronal circuit behavior in the vertebrate CNS. At fast inhibitory synapses, thought to occur by increasing number GABA<sub>A</sub> receptors (GABARs) same subunit composition preexisting synapses. Here, we show that mouse cerebellar granule cell GABAergic occurs different mechanism. Specifically, neuropeptide hormone, insulin, strengthens recruiting α6-containing GABARs rather than accumulating more α1-containing...
Windup is a progressive, frequency-dependent increase in the excitability of trigeminal and spinal dorsal horn wide dynamic range (WDR) nociceptive neurons to repetitive stimulation primary afferent C-fibers. Superficial neurokinin 1 receptor (NK1R)-expressing were recently shown regulate sensitization WDR through activation defined spino-bulbo-spinal loop. However, windup was not regulated this In present study, we sought identify alternative circuit activated by NK1Rs that mediates neuron...
Characterizing neurons by their electrophysiological phenotypes is essential for understanding the neural basis of behavioral and cognitive functions. Technological developments have enabled collection hundreds recordings; this calls new tools capable performing feature extraction efficiently. To address urgent need a powerful accessible tool, we developed ElecFeX, an open-source MATLAB-based toolbox that (1) has intuitive graphical user interface, (2) provides customizable measurements wide...
Spinal cord dorsal horn inhibition is critical to the processing of sensory inputs, and its impairment leads mechanical allodynia. How this decreased occurs whether restoration alleviates allodynic pain are poorly understood. Here, we show that a step in loss inhibitory tone change firing pattern parvalbumin (PV)-expressing neurons (PVNs). Our results PV, calcium-binding protein, controls activity PVNs by enabling them sustain high-frequency tonic patterns. Upon nerve injury, transition...
Spontaneous arrhythmia characterization in healthy rats can support interpretation when studying novel therapies. Male (n = 55) and female 40) Sprague-Dawley with telemetry transmitters for a derivation II ECG. Arrhythmias were assessed from continuous ECG monitoring over period of 24-48 h, data analyzed using an automated detection algorithm 100% manual over-read. While total 1825 spontaneous ventricular premature beats (VPB) identified, only 7 (or 7.4%) did not present any the recording...
Abstract Parvalbumin-expressing interneurons (PVINs) play a crucial role within the dorsal horn of spinal cord by preventing touch inputs from activating pain circuits. After nerve injury, their output is decreased via mechanisms that are not fully understood. In this study, we show PVINs nerve-injured mice change firing pattern tonic to adaptive. To examine ionic responsible for output, employed reparametrized Hodgkin-Huxley (HH) type model PVINs, which predicted (1) transition due an...
Characterization of the incidence spontaneous arrhythmias to identify possible drug-related effects is often an important part analysis in safety pharmacology studies using telemetry.A retrospective non-clinical species with and without telemetry transmitters was conducted. Electrocardiograms (24 h) from male female beagle dogs (n = 131), Göttingen minipigs 108) cynomolgus non-human primates (NHP; n 78) were analyzed.Ventricular tachycardia (VT) observed 3% but absent NHPs. Ventricular...
Abstract The nervous system processes sensory information by relying on the precise coordination of neuronal networks and their specific synaptic firing patterns. In spinal cord, disturbances to pattern tonic parvalbumin (PV)-expressing inhibitory interneuron (PV neurons) disrupt ability dorsal horn integrate touch may result in pathological phenotypes. protein (PVp) is a calcium (Ca 2+ )-binding that buffers accumulation Ca following train action potential allow for firing. Here, we find...
Introduction: Corrected QT (QTc) interval is an essential proarrhythmic risk biomarker, but recent data have identified limitations to its use. The J T-peak (JTp) alternative biomarker for evaluating drug-induced risk. aim of this study was evaluate pharmacological effects using spatial magnitude leads and DII electrocardiogram (ECG) common ECG confounders (ie, stress body temperature changes) on covariate adjusted (QTca), JTp (JTpca), T-end (Tpeca) intervals. Methods: Beagle dogs were...
Pre-clinically, safety risk assessment of a drug is primarily tested in vivo using functional evaluation adult animals while the mechanistic etiology drug-induced CNS adverse effects often uncharacterized. In vitro electrophysiology may provide better understanding without additional animal use. However, protocols are typically designed for embryonic or juvenile animals. We examined whether brain tissue isolated from rats (3–5 months old) and non-human primates (NHPs) (2–8 years can generate...
Motivation Profiling neurons by their electrophysiological phenotype is essential for understanding roles in information coding within and beyond the nervous systems. Technological development has unleashed our power to record more than ever before, yet booming size of dataset poses new challenges data analysis. Current software tools require users have either significant programming knowledge or devote great time effort, which impedes prevalence adoption among experimentalists. To address...
Characterizing neurons by their electrophysiological phenotypes is essential for understanding the neural basis of behavioral and cognitive functions. Technological developments have enabled collection hundreds recordings; that calls new toolkits capable performing feature extraction efficiently. To address urgent need a powerful accessible tool, we developed ElecFeX, an open-source MATLAB-based toolbox (1) has intuitive graphical user interface, (2) provides customizable measurements...