A5081880237- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- CAR-T cell therapy research
- Cytomegalovirus and herpesvirus research
- vaccines and immunoinformatics approaches
- Influenza Virus Research Studies
- Immune Response and Inflammation
- Cancer Immunotherapy and Biomarkers
- Glutathione Transferases and Polymorphisms
- SARS-CoV-2 and COVID-19 Research
- Glycosylation and Glycoproteins Research
- Cancer, Hypoxia, and Metabolism
- Cell Adhesion Molecules Research
- Celiac Disease Research and Management
- Reproductive System and Pregnancy
- HIV Research and Treatment
- Galectins and Cancer Biology
- Toxin Mechanisms and Immunotoxins
- Cytokine Signaling Pathways and Interactions
- Peptidase Inhibition and Analysis
- Enzyme Structure and Function
- Microscopic Colitis
- Diabetes and associated disorders
Monash University
2016-2025
Cardiff University
2016-2025
Australian Regenerative Medicine Institute
2016-2025
Institute of Infection and Immunity
2014-2025
Australian Research Council
2015-2024
University Hospital of Wales
2023-2024
ARC Centre of Excellence in Advanced Molecular Imaging
2011-2023
Discovery Institute
2016-2023
Clayton Foundation
2020
ORCID
2020
Mucosal-associated invariant T cells (MAIT cells) express a semi-invariant cell receptor (TCR) α-chain, TRAV1-2–TRAJ33, and are activated by vitamin B metabolites bound the major histocompatibility complex (MHC)–related class I–like molecule, MR1. Understanding MAIT biology has been restrained lack of reagents to specifically identify characterize these cells. Furthermore, use surrogate markers may misrepresent population. We show that modified human MR1 tetramers loaded with potent ligand,...
Celiac disease is a genetic condition that results in debilitating immune reaction the gut to antigens grain. The antigenic peptides recognized by T cells cause this are incompletely defined. Our understanding of epitopes pathogenic CD4+ based primarily on responses shown intestinal T-cells vitro hydrolysates or polypeptides gluten, causative antigen. A protease-resistant 33-amino acid peptide from wheat α-gliadin immunodominant antigen, but little known about spectrum cell rye and barley...
A surprising immune twist for RORC The system needs its full array of soldiers—including cells and the molecules they secrete—to optimally protect host. When this isn't case, minor infections can become chronic or even deadly. Markle et al. report discovery seven individuals carrying loss-of-function mutations in RORC, which encodes transcription factors RORγ RORγT. These lacked that produce cytokine interleukin-17, causing them to suffer from candidiasis. RORC-deficient also exhibited...
Rheumatoid arthritis (RA) is strongly associated with the human leukocyte antigen (HLA)-DRB1 locus that possesses shared susceptibility epitope (SE) and citrullination of self-antigens. We show how citrullinated aggrecan vimentin epitopes bind to HLA-DRB1*04:01/04. Citrulline was accommodated within electropositive P4 pocket HLA-DRB1*04:01/04, whereas electronegative RA-resistant HLA-DRB1*04:02 allomorph interacted arginine or citrulline-containing epitopes. Peptide elution studies revealed...
Studies on the biology of mucosal-associated invariant T cells (MAIT cells) in mice have been hampered by a lack specific reagents. Using MR1-antigen (Ag) tetramers that specifically bind to MR1-restricted MAIT cell receptors (TCRs), we demonstrate are detectable broad range tissues C57BL/6 and BALB/c mice. These include CD4−CD8−, CD4−CD8+, CD4+CD8− subsets, their frequency varies tissue- strain-specific manner. Mouse CD44hiCD62Llo memory phenotype produce high levels IL-17A, whereas other...
Obesity and type 2 diabetes (T2D) are associated with low-grade inflammation, activation of immune cells, alterations the gut microbiota. Mucosal-associated invariant T (MAIT) which innate-like cells that recognize bacterial ligands, present in blood enriched mucosal inflamed tissues. Here, we analyzed MAIT adipose tissues patients T2D and/or severe obesity. We determined circulating cell frequency was dramatically decreased both patient groups, this population even undetectable some obese...
Mucosal-associated invariant T (MAIT) cells express an cell receptor (TCR) α-chain (TRAV1-2 joined to TRAJ33, TRAJ20, or TRAJ12 in humans), which pairs with array of TCR β-chains. MAIT TCRs can bind folate- and riboflavin-based metabolites restricted by the major histocompatibility complex (MHC)-related class I−like molecule, MR1. However, impact MR1-ligand heterogeneity on biology is unclear. We show how a previously uncharacterized MR1 ligand, acetyl-6-formylpterin (Ac-6-FP), markedly...