A5002908830- vaccines and immunoinformatics approaches
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Drug-Induced Adverse Reactions
- Monoclonal and Polyclonal Antibodies Research
- Urticaria and Related Conditions
- CAR-T cell therapy research
- Peptidase Inhibition and Analysis
- Influenza Virus Research Studies
- Autoimmune Bullous Skin Diseases
- HIV Research and Treatment
- Asthma and respiratory diseases
- Mast cells and histamine
- Cancer Immunotherapy and Biomarkers
- Cytomegalovirus and herpesvirus research
- Glycosylation and Glycoproteins Research
- Contact Dermatitis and Allergies
- SARS-CoV-2 and COVID-19 Research
- Bioinformatics and Genomic Networks
- Advanced Proteomics Techniques and Applications
- Viral Infections and Outbreaks Research
- Platelet Disorders and Treatments
- Eosinophilic Disorders and Syndromes
- Biosensors and Analytical Detection
Australian Regenerative Medicine Institute
2016-2025
Monash University
2016-2025
Discovery Institute
2023-2025
Institute of Infection and Immunity
2024
The University of Melbourne
2010-2014
Biotechnology Institute
2012
Mucosal-associated invariant T (MAIT) cells express a semiinvariant αβ cell receptor (TCR) that binds MHC class I-like molecule (MR1). However, the molecular basis for MAIT TCR recognition by MR1 is unknown. In this study, we present crystal structure of human Vα7.2Jα33-Vβ2 TCR. Mutagenesis revealed highly conserved requirements TCR-MR1 interaction across different TCRs stimulated distinct microbial sources. Individual residues within β chain were dispensable with MR1, whereas α controlled...
The diversity of peptides displayed by class I human leukocyte antigen (HLA) plays an essential role in T cell immunity. peptide repertoire is extended various posttranslational modifications, including proteasomal splicing fragments from distinct regions to form nongenomically templated cis-spliced sequences. Previously, it has been suggested that a fraction the immunopeptidome constitutes such peptides; however, because computational limitations, not possible assess whether trans-spliced...
Manipulation of the MHC-I presentation pathway, and thus limiting cell surface expression, is used by many viruses to evade immune recognition. In particular, downregulation molecules at can reduce ability CD8+ T cells recognise viral peptides presented thereby delay clearance cells. To date, influenza has not been reported. Given that virus infections are a global health concern play an important role in promoting recovery from disease, we investigated whether A B (IAV, IBV) downregulated...
Natural killer (NK) cell activation is regulated by immunoglobulin-like receptors (KIRs) that recognize human leukocyte antigen (HLA) class I molecules. While polymorphism in HLA can directly impact these interactions, the extent to which HLA-associated peptide repertoire modulates NK function less well understood. Therefore, requirements for recognition of 2 ligands, HLA-B*57:01 and HLA-A*24:02, share similar KIR3DL1 binding residues but differ their capacity inhibit cells were assessed....
Volcano and other analytical plots (e.g., correlation plots, upset heatmaps) serve as important data visualization methods for transcriptomic proteomic analyses. Customizable generation of these is fundamentally a better understanding dysregulated expression therefore instrumental the ensuing pathway analysis biomarker identification. Here, we present an R-based Shiny application, termed ggVolcanoR, to allow customizable volcano heatmaps differential datasets, via user-friendly interactive...
Type 1 diabetes is characterized by the autoimmune destruction of pancreatic β-cells. Recognition major histocompatibility complex (MHC)-bound peptides critical for both initiation and progression disease. In this study, MHC peptide complexes were purified from NIT-1 β-cells, interferon-γ (IFN-γ)-treated cells, splenic thymic tissue 12-week-old NOD mice, identified mass spectrometry. addition to global liquid chromatography-tandem spectrometry analysis, targeted approach multiple-reaction...
Neopeptide-based immunotherapy has been recognised as a promising approach for the treatment of cancers. For neopeptides to be by CD8+ T cells and induce an immune response, their binding human leukocyte antigen class I (HLA-I) molecules is necessary first step. Most epitope prediction tools thus rely on such binding. With use mass spectrometry, scale naturally presented HLA ligands that could used develop predictors expanded. However, there are rarely efforts focus integration these...
Abstract Indigenous people worldwide are at high risk of developing severe influenza disease. HLA-A*24:02 allele, highly prevalent in populations, is associated with influenza-induced mortality, although the basis for this association unclear. Here, we define CD8 + T-cell immune landscapes against A (IAV) and B (IBV) viruses HLA-A*24:02-expressing non-Indigenous individuals, human tissues, influenza-infected patients HLA-A*24:02-transgenic mice. We identify immunodominant protective...
Background Major histocompatibility complex class-1-related protein (MR1), unlike human leukocyte antigen (HLA) class-1, was until recently considered to be monomorphic. MR1 presents metabolites in the context of host responses bacterial infection. MR1-restricted TCRs specific tumor cells have been described, raising interest their potential therapeutic application for cancer treatment. The diversity MR1-ligand biology has broadened with observation that single nucleotide variants (SNVs)...
Abstract The major histocompatibility complex (MHC) encodes a range of immune response genes, including the human leukocyte antigens (HLAs) in humans. These molecules bind peptide and present them on cell surface for T recognition. repertoires peptides presented by HLA are termed immunopeptidomes. highly polymorphic nature genres that encode confers allotype-specific differences sequences bound ligands. Allotype-specific ligand preferences often defined peptide-binding motifs. Individuals...
Immunophenotypic differences between closely related human leukocyte antigen (HLA) alleles have been associated with divergent clinical outcomes in infection, autoimmunity, transplantation and drug hypersensitivity. Here we explore the impact of micropolymorphism on peptide presentation by three HLA molecules, HLA-B*57:01, HLA-B*57:03 HLA-B*58:01, that are differentially HIV elite controller phenotype adverse reactions. For each allotype, mine ligand data sets derived from same parental cell...
The presentation of post-translationally modified (PTM) peptides by cell surface HLA molecules has the potential to increase diversity targets for surveilling T cells. Although immunopeptidomics studies routinely identify thousands HLA-bound from lines and tissue samples, in-depth analyses proportion nature bearing one or more PTMs remains challenging. Here we have analyzed a variety allotypes assessed distribution mass spectrometry-detected PTMs, finding deamidation asparagine glutamine be...
HLA-A*11:01 is one of the most prevalent human leukocyte antigens (HLAs), especially in East Asian and Oceanian populations. It also highly expressed Indigenous people who are at high risk severe influenza disease. As CD8 + T cells can provide broadly cross-reactive immunity to distinct strains subtypes, including A, B C viruses, understanding cell viruses across prominent HLA types needed rationally design a universal vaccine generate protective for high-risk only handful...