A5038494526- Escherichia coli research studies
- Endoplasmic Reticulum Stress and Disease
- Viral gastroenteritis research and epidemiology
- Clostridium difficile and Clostridium perfringens research
- Transgenic Plants and Applications
- Bacterial Genetics and Biotechnology
- Autophagy in Disease and Therapy
- Heat shock proteins research
- Toxin Mechanisms and Immunotoxins
- Bacteriophages and microbial interactions
- Glycosylation and Glycoproteins Research
- Pancreatic function and diabetes
- RNA regulation and disease
- Pneumonia and Respiratory Infections
- Immune Cell Function and Interaction
- Cellular transport and secretion
- Monoclonal and Polyclonal Antibodies Research
- Streptococcal Infections and Treatments
- Diabetes and associated disorders
- Vibrio bacteria research studies
- Probiotics and Fermented Foods
- Cytomegalovirus and herpesvirus research
- Microbial Inactivation Methods
- Bacterial Infections and Vaccines
- Gut microbiota and health
The University of Adelaide
2016-2025
Centre National de la Recherche Scientifique
2024
Centre d’Immunologie de Marseille-Luminy
2024
Nirma (India)
2012
University of Tübingen
2011
Arizona State University
2011
Monash University
2009
The University of Melbourne
2009
Michigan State University
2009
Women's and Children's Hospital
1992-2001
ABSTRACT Shiga toxigenic Escherichia coli (STEC) comprises a diverse group of organisms capable causing severe gastrointestinal disease in humans. Within the STEC family, certain strains appear to be greater virulence for humans, example, those belonging serogroups O111 and O157 with particular combinations other putative traits. We have developed two multiplex PCR assays detection genetic characterization cultures feces or foodstuffs. Assay 1 utilizes four primer pairs detects presence stx...
Protein folding by the endoplasmic reticulum (ER) is physiologically critical; its disruption causes ER stress and augments disease. activates unfolded protein response (UPR) to restore homeostasis. If persists, UPR induces apoptotic cell death, but mechanisms remain elusive. Here, we report that unmitigated promoted apoptosis through cell-autonomous, UPR-controlled activation of death receptor 5 (DR5). stressors induced DR5 transcription via mediator CHOP; however, sensor IRE1α transiently...
Shiga toxin has the potential to induce expression of inflammation-associated genes, although underlying mechanisms are not well understood. We examined effects subtilase cytotoxin (SubAB), an AB(5) produced by some toxigenic Escherichia coli, on activation NF-kappaB. SubAB is known be a protease which selectively degrades GRP78/Bip. Treatment NRK-52E cells with caused rapid cleavage GRP78. Following degradation GRP78, transient NF-kappaB was observed peak at 6-12 h; subsided within 24 h...
Stem cells generate rapidly dividing transit-amplifying that have lost the capacity for self-renewal but cycle a number of times until they exit cell and undergo terminal differentiation. We know very little type signals trigger earliest steps stem differentiation mediate to transition. show in normal intestinal epithelium, endoplasmic reticulum (ER) stress activity unfolded protein response (UPR) are induced at transition from cell. Induction ER causes loss stemness Perk-eIF2α-dependent...
ABSTRACT The capacity of Shiga toxigenic Escherichia coli (STEC) to adhere the intestinal mucosa undoubtedly contributes pathogenesis human disease. majority STEC strains isolated from severe cases produce attaching and effacing lesions on mucosa, a property mediated by locus enterocyte effacement (LEE) pathogenicity island. This element is not essential for pathogenesis, as some disease, including hemolytic uremic syndrome (HUS), are caused LEE-negative strains, but mechanism whereby these...
ABSTRACT We recently described a novel megaplasmid-encoded adhesin produced by certain Shiga toxigenic Escherichia coli (STEC) strains that lack the locus for enterocyte effacement (LEE) pathogenicity island. This adhesin, designated Saa (STEC autoagglutinating adhesin), may be marker subset of LEE-negative STEC capable causing severe gastrointestinal and systemic diseases in humans. In this study, we developed pentavalent PCR assay detection saa as well other proven putative virulence genes...
Shiga-like toxin-producing Escherichia coli (SLTEC) strains are a diverse group of organisms which known to cause diarrhea and hemorrhagic colitis in humans. This can lead potentially fatal systemic sequelae, such as hemolytic-uremic syndrome (HUS). Strains belonging more than 100 different O:H serotypes have been associated with severe SLTEC disease humans, only O157 (which uncommon Australia) distinguishable cultural characteristic (sorbitol negative). During an outbreak HUS Adelaide,...
The Shiga toxigenic Escherichia coli (STEC) O113:H21 strain 98NK2, which was responsible for an outbreak of hemolytic uremic syndrome, secretes a highly potent and lethal subtilase cytotoxin that is unrelated to any bacterial toxin described date. It the prototype new family AB5 toxins, comprising single 35-kilodalton (kD) A subunit pentamer 13-kD B subunits. subtilase-like serine protease distantly related BA_2875 gene product Bacillus anthracis. putative exported protein from Yersinia...
ABSTRACT Shiga toxigenic Escherichia coli (STEC) strains are a diverse group of organisms capable causing severe gastrointestinal disease in humans. Within the STEC family, certain appear to have greater virulence for carrying eae and belonging serogroup O157 or O111 been responsible vast majority outbreaks reported date. Here we describe O113:H21 strain lacking that was cluster three cases hemolytic-uremic syndrome. This produces single Stx2-related toxin adheres efficiently Henle 407 cells.
Subtilase cytotoxin (SubAB) is the prototype of a new family AB(5) cytotoxins produced by Shiga toxigenic Escherichia coli. Its cytotoxic activity due to its capacity enter cells and specifically cleave essential endoplasmic reticulum (ER) chaperone BiP (GRP78). In present study, we have examined trigger three ER stress-signalling pathways in Vero cells. Activation PKR-like kinase was demonstrated phosphorylation eIF2alpha, which occurred within 30 min toxin treatment, correlated with...
Japanese encephalitis virus (JEV), a mosquito-borne flavivirus, is the leading cause of viral in Southeast Asia with potential to become global pathogen. Here, we identify glucose-regulated protein 78 (GRP78) as an important host for entry and replication. Using plasma membrane fractions from mouse neuronal (Neuro2a) cells, mass spectroscopy analysis identified GRP78 interacting recombinant JEV envelope domain III. was found be expressed on membranes Neuro2a primary neurons, human epithelial...
Biosynthesis of insulin – critical to metabolic homeostasis begins with folding the proinsulin precursor, including formation three evolutionarily conserved intramolecular disulfide bonds. Remarkably, normal pancreatic islets contain a subset molecules bearing at least one free cysteine thiol. In human (or rodent) perturbed endoplasmic reticulum environment, non-native enters intermolecular disulfide-linked complexes. genetically obese mice otherwise wild-type islets, complexes are more...
ABSTRACT During infection, Streptococcus pneumoniae exists mainly in sessile biofilms rather than planktonic form, except during sepsis. The capacity to form is believed be important for nasopharyngeal colonization as well disease pathogenesis, but relatively little known about the regulation of this process. Here, we investigated effect exogenous iron [Fe(III)] role luxS (encoding S-ribosylhomocysteine lyase) on biofilm formation by S. D39. Fe(III) strongly enhanced at concentrations ≥50...
Abstract Mammals express the sialic acids N -acetylneuraminic acid (Neu5Ac) and N- glycolylneuraminic (Neu5Gc) on cell surfaces, where they act as receptors for pathogens, including influenza A virus (IAV). Neu5Gc is synthesized from Neu5Ac by enzyme cytidine monophosphate- hydroxylase (CMAH). In humans, this inactive only produced. Ferrets are susceptible to human-adapted IAV strains have been dominant animal model studies. Here we show that ferrets, like do not synthesize Neu5Gc. Genomic...
Significance The pneumococcus accounts for 25% of deaths in children under 5 y age developing countries. One the most important virulence factors expressed by this pathogen is pore-forming toxin, pneumolysin (Ply), an example a Gram-positive cholesterol-dependent cytolysin (CDC). We show that Ply interacts with Lewis histo-blood group antigen sialyl LewisX and blocking interaction can protect RBCs from lysis. also identify glycan receptors on CDC streptolysin O A streptococcus. Our study...
Ubiquitin ligases (E3s) embedded in the endoplasmic reticulum (ER) membrane regulate essential cellular activities including protein quality control, calcium flux, and sterol homeostasis. At least 25 different, transmembrane domain (TMD)-containing E3s are predicted to be ER-localised, but for most their organisation roles remain poorly defined. Using a comparative proteomic workflow, we mapped over 450 protein-protein interactions 21 stably expressed, full-length E3s. Bioinformatic analysis...