A5052411771- Zebrafish Biomedical Research Applications
- Neuroinflammation and Neurodegeneration Mechanisms
- Immune cells in cancer
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- DNA and Nucleic Acid Chemistry
- Microtubule and mitosis dynamics
- Cancer-related Molecular Pathways
- Immunotherapy and Immune Responses
- Bone Metabolism and Diseases
- Advanced Breast Cancer Therapies
- Ubiquitin and proteasome pathways
- Circular RNAs in diseases
- Immune Cell Function and Interaction
- Phagocytosis and Immune Regulation
- Gene expression and cancer classification
- Intensive Care Unit Cognitive Disorders
- ATP Synthase and ATPases Research
- Extracellular vesicles in disease
- Protein Kinase Regulation and GTPase Signaling
- Reproductive System and Pregnancy
- RNA modifications and cancer
- Melanoma and MAPK Pathways
- Bone health and treatments
- Invertebrate Immune Response Mechanisms
Quzhou City People's Hospital
2024
Wenzhou Medical University
2024
Harvard University
2021-2024
Brigham and Women's Hospital
2021-2024
Sun Yat-sen University
2023
Sun Yat-sen University Cancer Center
2023
Loxo Oncology at Lilly (United States)
2021
University of Rochester
2020
Michigan Technological University
2009-2020
Hong Kong University of Science and Technology
2018-2020
Loss-of-function mutations in the retinoblastoma gene RB1 are common several treatment-refractory cancers such as small-cell lung cancer and triple-negative breast cancer. To identify drugs synthetic lethal with mutation (RB1 mut), we tested 36 cell-cycle inhibitors using a cell panel profiling approach optimized to discern cytotoxic from cytostatic effects. Inhibitors of Aurora kinases AURKA AURKB showed strongest association this assay. LY3295668, an inhibitor over 1,000-fold selectivity...
Abstract Background Human carcinoembryonic antigen cell adhesion molecule 1 (CEACAM1) is an inhibitory surface protein that functions through homophilic and heterophilic ligand binding. Its expression on immune cells in human tumors poorly understood. Methods An antibody distinguishes CEACAM1 from other highly related CEACAM family members was labeled with 159 Tb inserted into a panel of antibodies included specificity for programmed death (PD1) PD-L1, which are targets immunotherapy, to...
Abstract Teriparatide, human PTH (1‐34), a new therapy for osteoporosis, elicits markedly different skeletal responses depending on the treatment regimen. In order to understand potential mechanisms this dichotomy, present investigation utilized microarrays delineate genes and pathways that are regulated by intermittent (subcutaneous injection of 80 μg/kg/day) continuous infusion 40 μg/kg/day osmotic mini pump) (1–34) 1 week in 6‐month‐old female rats. The effect each regimen was confirmed...
The origin of Langerhans cells (LCs), which are skin epidermis-resident macrophages, remains unclear. Current lineage tracing LCs largely relies on the promoter-Cre-LoxP system, often gives rise to contradictory conclusions with different promoters. Thus, reinvestigation an improved method is necessary. Here, using a laser-mediated temporal-spatial resolved cell labeling method, we demonstrated that most adult originated from ventral wall dorsal aorta (VDA), equivalent mouse aorta, gonads,...
Abstract KRAS-G12C is an important oncogenic mutation in patients with NSCLC, CRC, and other cancer types. Currently, there are no FDA-approved inhibitors, those clinical development have relatively modest activity compared to approved therapies targeting classic drivers. This may be potentially due part incomplete target occupancy trapping of mutant KRAS the inactive GDP-bound state. Achieving maximal benefit harboring a mutation, require potent inhibitor capable achieving near complete...
Abstract Endometrial cancer (EC), as one of the most common cancers, severely threatens female reproductive health. Our previous study has shown that Kinesin family member C1 (KIFC1) played crucial roles in progression EC. In addition, abnormal centrosome amplification, which was reported to be partially regulated by KIFC1, usually occurred different cancers. However, whether KIFC1 promoted EC through amplification and potential mechanism remain revealed. The present demonstrated...
Synthetic oligodeoxynucleotide is purified by capping failure sequences with an acrylated phosphoramidite followed polymerization and product extraction. The method suitable for large scale oligonucleotide drug purification.
Abstract Nitidine chloride (NC), a bioactive alkaloid isolated from Zanthoxylum nitidum , has been used as herbal ingredient in toothpaste that prevents cavities for decades. It also displays potential antitumor and anti-inflammation properties. However, its anticatabolic effect on bone is not known. We investigated the of NC osteoclastogenesis, resorption RANKL-induced NF-κB NFATc1 signalling. In mouse-derived marrow monocytes (BMMs), suppressed multinucleated tartrate-resistant acid...
Immune cells in the mucosal barriers of vertebrates are highly heterogeneous their origin and function. This heterogeneity is further exemplified by recent discovery ectoderm-derived immune cells—metaphocytes zebrafish epidermis. Yet, whether non-hematopoiesis-derived generally exist barrier tissues remains obscured. Here, we report identification characterization an endoderm-derived cell population gill intestine zebrafish. Transcriptome analysis reveals that myeloid-like with high...
The readily scalable catching by polymerization purification technology has been further advanced to purify 5′-phosphorylated synthetic oligodeoxynucleotides (ODNs). new utilizes a phosphoramidite that contains fluoride-cleavable diisopropylsilyl acetal linker and polymerizable methacrylamide group, is capable of phosphorylation ODN. For purification, the was coupled 5′-end full-length ODN on synthesizer. Because failure sequences were capped in each cycle, only phosphinylated acrylated....
By use of 1,3-dithian-2-yl-methoxycarbonyl (Dmoc) as a protecting group and linker for oligodeoxynucleotide (ODN) synthesis, deprotection cleavage are achieved under non-nucleophilic oxidative conditions. The nucleophile-sensitive thioester α-chloroacetyl groups conveniently incorporated into ODN sequences. technology could be universally useful electrophilic synthesis.
Doxorubicin (Dox) is a widely used clinical drug whose cardiotoxicity cannot be ignored. Pyroptosis (inflammatory cell death) has gradually gained attention in the context of Dox-induced cardiotoxicity. In addition to inhibition platelet activation by ticagrelor, little known about its other pharmacological effects. Glycogen synthase kinase 3β (GSK-3β) been shown contribute pathological process pyroptosis, but whether it related potential role ticagrelor unclear. this study, we investigated...
Microglia are tissue-resident macrophages in the central nervous system (CNS) that play essential roles regulation of CNS development and homeostasis. Yet, genetic networks governing microglia remain incompletely defined. Here, we report identification characterization a microglia-defective zebrafish mutant wulonghkz12 (wulhkz12) isolated from an ethylnitrosourea (ENU)-based screen. We show wulhkz12 mutants harbors missense point mutation gene region encoding PRY/SPRY domain tripartite-motif...
Tissue-resident macrophages (TRMs), generally found in tissues under normal physiological conditions, play crucial roles not only immunity but also tissue development and homeostasis. Because of their diverse functions, dysregulation function has been implicated many human disorders. In the past decade, a great deal extensive studies have conducted various model organisms with cutting-edge technologies to explore origin TRMs. this review, we summarize recent findings on TRMs mouse zebrafish...
<h2>Summary</h2> Metaphocytes are tissue-resident macrophage (TRM)/dendritic cell (DC)-like cells of non-hematopoietic origin in zebrafish barrier tissues. One remarkable property metaphocytes is their ability to capture soluble antigens from the external environment via transepithelial protrusions, a unique function manifested by specialized subpopulations TRMs/DCs mammal Yet, how acquire myeloid-like properties precursors and they regulate immunity remains unknown. Here, we show that <i>in...