A5007299404- Estrogen and related hormone effects
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- RNA Interference and Gene Delivery
- Cytokine Signaling Pathways and Interactions
- Bone Metabolism and Diseases
- Bone Tissue Engineering Materials
- Bone health and osteoporosis research
- RNA regulation and disease
- Sarcoma Diagnosis and Treatment
- Bone health and treatments
- RNA and protein synthesis mechanisms
- interferon and immune responses
- Cancer-related gene regulation
- Wnt/β-catenin signaling in development and cancer
- Nanoparticle-Based Drug Delivery
- Autophagy in Disease and Therapy
- Virus-based gene therapy research
- Advanced biosensing and bioanalysis techniques
- DNA and Nucleic Acid Chemistry
- Spine and Intervertebral Disc Pathology
- Chronic Myeloid Leukemia Treatments
- Dental Implant Techniques and Outcomes
- MicroRNA in disease regulation
- Eosinophilic Disorders and Syndromes
Mayo Clinic in Arizona
2003-2021
Mayo Clinic
2008-2020
Mayo Clinic in Florida
2001-2020
WinnMed
2004-2019
Paracelsus Medical University
2012
University of Rochester
2012
National Institutes of Health
1995-1998
National Institute of Diabetes and Digestive and Kidney Diseases
1997-1998
Cleveland Clinic
1993-1998
Long Island Jewish Medical Center
1990-1995
Activation of 2-5A-dependent ribonuclease by 5′-phosphorylated, 2′,5′-linked oligoadenylates, known as 2-5A, is one pathway interferon action. Unaided uptake into HeLa cells 2-5A linked to an antisense oligonucleotide resulted in the selective ablation messenger RNA for double-stranded (dsRNA)-dependent protein kinase PKR. Similarly, purified, recombinant human was induced selectively cleave PKR RNA. Cells depleted activity were unresponsive activation nuclear factor-κB (NF-κB) dsRNA...
Trans-3,4,5-trihydroxystilbene (resveratrol), a polyphenolic compound found in juice and wine from dark-skinned grape cultivars, was recently shown to bind estrogen receptors vitro, where it activated transcription of estrogen-responsive reporter genes. The purpose this 6-day study weanling rats determine the dose response (1, 4, 10, 40, 100 μg/day) effects orally administered resveratrol on target tissues. solvent (10% ethanol) had no significant effect any measurement or derived value....
Abstract Teriparatide, human PTH (1‐34), a new therapy for osteoporosis, elicits markedly different skeletal responses depending on the treatment regimen. In order to understand potential mechanisms this dichotomy, present investigation utilized microarrays delineate genes and pathways that are regulated by intermittent (subcutaneous injection of 80 μg/kg/day) continuous infusion 40 μg/kg/day osmotic mini pump) (1–34) 1 week in 6‐month‐old female rats. The effect each regimen was confirmed...
Osteosarcoma is the most common bone cancer. Despite advances, molecular mechanisms associated with osteosarcoma have not been fully understood. Hence, an effective treatment for has yet to be developed. Even though signal transducer and activator of transcription3 (STAT3) implicated, its role in pathogenesis determined. In this study, we investigated antitumor effect napabucasin (NP) (BBI608), inhibitor STAT3 on vitro vivo studied underlying mechanism. Cell viability, colony formation,...
There is no animal model that reflects the histological and radiographical heterogeneity of osteosarcoma. We assessed seven osteosarcoma cell lines for their potential to develop orthotopic tumors lung metastasis in SCID mice. Whereas radiologically, 143B developed osteolytic tumors, SaOS-LM7 osteoblastic primary tumors. The mineralization status was confirmed by assessing alkaline phosphatase activity microarray expression profile. herein report a xenograft mouse assess lesions, which may...
Abstract Well‐differentiated liposarcoma (WDLS) is one of the most common malignant mesenchymal tumors and dedifferentiated (DDLS) a tumor consisting both WDLS transformed nonlipogenic sarcomatous component. Cytogenetically, characterized by presence ring or giant rod chromosomes containing several amplified genes, including MDM2 , TSPAN31, CDK4, others mainly derived from chromosome bands 12q13‐15. However, 12q13‐15 amplicon large discontinuous. The focus this study was to identify novel...
Bone morphogenetic proteins (BMPs) play a central role in local bone regeneration strategies, whereas the anabolic features of parathyroid hormone (PTH) are particularly appealing for systemic treatment generalized loss. The aim current study was to investigate whether BMP-2-induced could be enhanced by administration PTH (1-34). Empty or BMP-2-loaded poly(lactic-co glycolic acid)/poly(propylene fumarate)/gelatin composites were implanted subcutaneously and femoral defects rats (n = 9). For...
Alcohol is a risk factor for the development of osteoporosis, especially in men. Chronic alcohol abuse decreases bone mass, which contributes to increased incidence fractures. To better understand mechanism action ethanol on metabolism, we have studied dose-response effects conditionally immortalized human fetal osteoblasts (hFOB) culture. Ethanol treatment had no significant osteoblast number after 1 day or 7 days. did not reduce type I collagen protein levels at either time point any dose...
Abnormal secretion of PTH by the parathyroid glands contributes to a variety common skeletal disorders. Prior studies implicate platelet-derived growth factor-A (PDGF-A) as an important mediator selective actions on bone. The present used targeted gene profiling and small-molecule antagonists directed against candidate products elucidate roles specific PTH-regulated genes signaling pathways. A group 29 in rats continuously infused with cotreated PDGF receptor antagonist trapidil were...
Osteosarcoma is the most common primary malignant bone tumor in children and young adults. Surgical resection adjunctive chemotherapy are only widely available options of treatment for this disease. Anti-tumor compound 2-Methoxyestradiol (2-ME) triggers cell death through induction apoptosis osteosarcoma cells, but not normal osteoblasts. In report, we have investigated whether autophagy plays a role 2-ME actions on cells. Transmission electron microscopy imaging shows that leads to...