The posterior inclination of the tibial plateau, which is referred to as slope, determined routinely on lateral radiographs. However, radiographically, it not always possible reliably recognize making a separate assessment medial and plateaus difficult. We propose technique measure separately by defining longitudinal axis conventional MRI. plateau slope obtained from radiographs was compared with MR images in 100 consecutive patients knee pain when ligament or meniscal injury assumed. MRI...

To investigate the predictive value of magnetic resonance (MR) imaging abnormalities lumbar intervertebral disks, particularly with adjacent endplate changes, to predict symptomatic disk derangement, discography as standard.Fifty patients aged 28-50 years chronic low back pain and without radicular leg underwent prospective clinical examination sagittal T1- T2-weighted transverse MR imaging. Subsequently, a provocation test (116 disks). images were evaluated for degeneration,...

Abstract Purpose: Tumor markers ideally allow monitoring and prediction of disease progression. In Ewing’s sarcoma, a devastating childhood cancer, only few reliable prognostic have been identified. To this end, we analyzed the expression four tumor-promoting proteins, cyclin D1, HER2/Neu, Mdm2, vascular endothelial growth factor (VEGF), in sarcoma. Experimental Design Results: Thirty-one tissue samples from patients with sarcoma were stained antibodies against or VEGF. Whereas no...

Chronic rotator cuff tendon tears lead to fatty infiltration and muscle atrophy with impaired physiological functions of the affected muscles. However, cellular molecular mechanisms corresponding pathophysiological processes remain unknown. The purpose this study was characterize expression pattern adipogenic (PPARgamma, C/EBPbeta) myogenic (myostatin, myogenin, Myf-5) transcription factors in infraspinatus sheep after tenotomy, implantation a tension device, refixation tendon,...

Formation of metastases in the lungs is major cause death patients suffering from osteosarcoma (OS). Metastases at presentation and poor response to preoperative chemotherapy are strong predictors for patient outcome. The elucidation molecular markers that promote metastasis formation and/or chemoresistance therefore importance. CD44 a plasma membrane glycoprotein binds extracellular matrix component hyaluronan (HA) has been shown be involved variety other tumors. Here we investigated role...

Abstract Osteosarcoma is the most frequent primary malignant bone tumor in children and adolescents with a high propensity for lung metastasis, major cause of disease-related death. Reliable outcome-predictive markers targets osteosarcoma metastasis-suppressing drugs are urgently needed more effective treatment metastasizing osteosarcoma, which has current mean 5-year survival rate approximately 20%. This study investigated prognostic value biological relevance extracellular...

The spectrum of mesenchymal tumors associated with rearrangements the EWSR1 gene has been growing in recent years due to progress molecular detection techniques. Originally identified as involved pathogenesis Ewing sarcoma, is now known be rearranged diverse clinical and histopathological entities. NFATC2 one many translocation partners fusions a morphologically typical, albeit rare, subgroup tumors. Little about characteristics containing since most few reports published describe rather...

Tumor-secreted extracellular vesicles (EVs) have been identified as mediators of cancer–host intercellular communication and shown to support pre-metastatic niche formation by modulating stromal cells at future metastatic sites. While osteosarcoma, the most common primary malignant bone tumor in children adolescents, has a high propensity for pulmonary metastases, interaction osteosarcoma with resident lung remains poorly understood. Here, we deliver foundational vitro evidence that...

Abstract Osteosarcoma is the most frequent malignant bone tumor with a poor survival rate for patients metastasis. Previous studies have shown that beside other proteases, distinct sets of cathepsins are involved in process metastasis different tumors. In this study we investigated expression cathepsin proteases human osteosarcoma First, mRNA 14 was studied SAOS‐2 cells and highly metastatic LM5 LM7 sublines by reverse transcriptase (RT)‐polymerase chain reaction (PCR). The D, K, L found...

There is no animal model that reflects the histological and radiographical heterogeneity of osteosarcoma. We assessed seven osteosarcoma cell lines for their potential to develop orthotopic tumors lung metastasis in SCID mice. Whereas radiologically, 143B developed osteolytic tumors, SaOS-LM7 osteoblastic primary tumors. The mineralization status was confirmed by assessing alkaline phosphatase activity microarray expression profile. herein report a xenograft mouse assess lesions, which may...

Metastasizing osteosarcoma has a mean 5-year survival rate of only 20% to 30%. Therefore, novel chemotherapeutics for more effective treatment this disease are required.The antineoplastic activity honokiol, which was demonstrated previously in numerous malignancies, studied vivo C3H mice subcutaneously injected with syngeneic β-galactosidase bacterial gene (lacZ)-expressing LM8 (LM8-lacZ) cells. In vitro cytotoxic effects honokiol were investigated 8 human and 2 murine cell lines different...

Abstract Aneurysmal bone cyst (ABC), once considered a reactive lesion, has been proven to be neoplasia characterized by rearrangements of the USP6 -gene. Aggressive local growth and recurrences are common therapeutic options may limited due vicinity crucial structures. We describe case locally aggressive, multinucleated giant cell-containing lesion forearm 21-year old woman, treated with denosumab for recurrent, surgically uncontrollable disease. Under influence this RANKL inhibitor, tumor...

ABSTRACT Chondrosarcoma is the second most frequent bone sarcoma. Due to inherent chemotherapy and radiotherapy resistance absence of known therapeutic targets, clinical management limited surgical resection. Consequently, patients with advanced disease face a poor prognosis. Hence, elucidating regulatory networks governing chondrosarcoma pathogenesis vital for development effective strategies. Here, miRNA mRNA next generation sequencing different subtypes human chondrogenic tumors in...

Skeletal muscle atrophy and fatty infiltration develop after tendon tearing. The extent of serves as one prognostic factor for the outcome surgical repair rotator cuff tears. We asked whether mRNA genes involved in regulation degradative processes leading to atrophy, ie, FOXOs, MSTN, calpains, cathepsins, transcripts ubiquitin-proteasome pathway, are overexpressed supraspinatus patients with without evaluated biopsy specimens collected during surgery 53 consecutive different sizes tears six...

Physeal distraction facilitates metaphyseal bone tumor resection in children and preserves the adjacent joint. The technique was first described by Cañadell. Tumor procedures allowing limb-sparing reconstruction have been used increasingly recent years without compromising oncologic principles.We report our results with Cañadell's assessing control, functional outcome, complications.Six consecutive primary malignant tumors underwent physeal as a part of resection. location distal femur four...

Primary bone cancers are among the deadliest cancer types in adolescents, with osteosarcomas being most prevalent form. Osteosarcomas commonly treated multi-drug neoadjuvant chemotherapy and therapy success as well patient survival is affected by presence of tumor suppressors. In order to assess prognostic value tumor-suppressive biomarkers, primary osteosarcoma tissues were analyzed prior after chemotherapy.We constructed a tissue microarray from high grade samples, consisting 48 naïve...

Osteosarcoma is a rare but highly malignant cancer of the bone. As consequence, number established cell lines used for experimental in vitro and vivo osteosarcoma research limited value these relies on their stability during culture. Here we investigated gene expression by microarray analysis array genomic hybridization three low metastatic derivatives thereof with increased potential using cells different passages.The showed altered culture, it was more pronounced two compared to respective...

<b><i>Background:</i></b> There is no established systemic treatment option for unresectable osteosarcoma progressing after standard chemotherapy. A recently published clinical trial has demonstrated some activity of sorafenib in this situation. Preclinical research suggests a role the inhibition receptor activator nuclear factor-ĸB ligand (RANKL), but data have been reported so far. <b><i>Case Report:</i></b> 37-year-old man was...

Current osteosarcoma therapies cause severe treatment-related side effects and chemoresistance, have low success rates. Consequently, alternative treatment options are urgently needed. Photodynamic therapy (PDT) is a minimally invasive, local with proven clinical efficacy for variety of tumor types. PDT cytotoxic, provokes anti-vascular stimulates cell targeting mechanisms the immune system and, consequently, has potential as novel patients. This study investigated uptake dark- phototoxicity...