A5086884279- Cancer Cells and Metastasis
- Sarcoma Diagnosis and Treatment
- Glycosylation and Glycoproteins Research
- Cell Adhesion Molecules Research
- interferon and immune responses
- Proteoglycans and glycosaminoglycans research
- Bone Tumor Diagnosis and Treatments
- Chemokine receptors and signaling
- Immunotherapy and Immune Responses
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Cancer Genomics and Diagnostics
- Cancer, Hypoxia, and Metabolism
- Extracellular vesicles in disease
- CRISPR and Genetic Engineering
- HER2/EGFR in Cancer Research
- Cancer Research and Treatments
- Monoclonal and Polyclonal Antibodies Research
- Cardiac tumors and thrombi
- Circadian rhythm and melatonin
- Metastasis and carcinoma case studies
- TGF-β signaling in diseases
- Virus-based gene therapy research
- Pancreatic and Hepatic Oncology Research
- Renal and related cancers
École Polytechnique Fédérale de Lausanne
2025
ETH Zurich
2020-2024
Universitätsklinik Balgrist
2012-2020
Institute for Biomedical Engineering
2020
University of Zurich
2013-2014
Abstract The presence of circulating tumor cell (CTC) clusters is associated with disease progression and reduced survival in a variety cancer types. In breast cancer, preclinical studies showed that inhibitors the Na + /K ATPase suppress CTC block metastasis. Here we conducted prospective, open-label, proof-of-concept study women metastatic where primary objective was to determine whether treatment inhibitor digoxin could reduce mean cluster size. An analysis nine patients treated daily...
Abstract Circulating tumor cells (CTCs) play a pivotal role in metastasis, the leading cause of cancer-associated death. Recent improvements CTC isolation tools, coupled with steady development multiomics technologies at single-cell resolution, have enabled an extensive exploration biology, unlocking insights into their molecular profiles. A detailed portrait requires interrogation across various levels encompassing genomic, epigenetic, transcriptomic, proteomic and metabolic features. Here,...
Current combined surgical and neo-adjuvant chemotherapy of primary metastatic osteosarcoma (OS) is ineffective, reflected by a 5-year survival rate affected patients less than 20 %. Studies in experimental OS metastasis models pointed to the CXCR4/CXCL12 homing axis as novel target for metastasis-suppressive treatment. The present study investigated first time CXCR4-blocking principle spontaneously metastasizing human 143B cell line-derived orthotopic xenograft mouse model. highly cells,...
Formation of metastases in the lungs is major cause death patients suffering from osteosarcoma (OS). Metastases at presentation and poor response to preoperative chemotherapy are strong predictors for patient outcome. The elucidation molecular markers that promote metastasis formation and/or chemoresistance therefore importance. CD44 a plasma membrane glycoprotein binds extracellular matrix component hyaluronan (HA) has been shown be involved variety other tumors. Here we investigated role...
Tumor-secreted extracellular vesicles (EVs) have been identified as mediators of cancer–host intercellular communication and shown to support pre-metastatic niche formation by modulating stromal cells at future metastatic sites. While osteosarcoma, the most common primary malignant bone tumor in children adolescents, has a high propensity for pulmonary metastases, interaction osteosarcoma with resident lung remains poorly understood. Here, we deliver foundational vitro evidence that...
ABSTRACT Chondrosarcoma is the second most frequent bone sarcoma. Due to inherent chemotherapy and radiotherapy resistance absence of known therapeutic targets, clinical management limited surgical resection. Consequently, patients with advanced disease face a poor prognosis. Hence, elucidating regulatory networks governing chondrosarcoma pathogenesis vital for development effective strategies. Here, miRNA mRNA next generation sequencing different subtypes human chondrogenic tumors in...
// Ana Gvozdenovic 1 , Aleksandar Boro Daniela Meier Beata Bode-Lesniewska 2 Walter Born Roman Muff Bruno Fuchs Laboratory for Orthopedic Research, Department of Orthopedics, Balgrist University Hospital, Zurich, Switzerland Pathology, Institute Surgical Hospital Correspondence to: Fuchs, email: bfuchs@research.balgrist.ch Keywords: integrins, metastasis, osteosarcoma, targeted therapy, cilengitide Received: February 04, 2016 Accepted: June 17, Published: July 07, ABSTRACT Osteosarcoma is an...
The pre-metastatic niche (PMN) is a tumor-driven microenvironment in distant organs that can foster and support the survival growth of disseminated tumor cells. This facilitates establishment secondary lesions eventually form overt metastasis, main cause cancer-related death. In recent years, tumor-derived extracellular-vesicles (EVs) have emerged as potentially key drivers PMN. role PMN osteosarcoma metastasis poorly understood potential contribution cell-derived EVs to formation has not...
More effective treatment of metastasizing osteosarcoma with a current mean 5-year survival rate less than 20% requires more detailed knowledge on mechanisms and key regulatory molecules the complex metastatic process. CXCR4, receptor chemokine CXCL12, has been reported to promote tumor progression metastasis in osteosarcoma. CXCR7 is recently deorphanized CXCL12-scavenging so far not well-defined functions biology. The present study focused potential malignancy enhancing function interaction...
Osteosarcoma (OS) is the most frequent primary malignant bone cancer in children and adolescents with a high propensity for lung metastasis. Therefore, it of great importance to identify molecular markers leading increased metastatic potential order devise more effective therapeutic strategies that suppress metastasis, major cause death OS. CD44, principal receptor extracellular matrix component hyaluronan (HA), frequently found overexpressed tumor cells has been implicated spread various...
The aim of this study was to characterize the different phenotypes osteosarcoma by PET, comparing uptake 3 tracers (<sup>18</sup>F-FDG, <sup>18</sup>F-fluoromisonidazole [<sup>18</sup>F-FMISO], and <sup>18</sup>F-fluoride) in preclinical mouse models that reflect heterogeneity human disease. <b>Methods:</b> Mouse LM8 osteosarcoma, 143B, Caprin-1 stably overexpressing SaOS-2 cells were injected intratibially C3H severe-combined immunodeficient mice. PET imaging with <sup>18</sup>F-FDG,...
Abstract Osteosarcoma is a cancer of pathological bone remodeling with high mortality and severe comorbidity. New therapies are urgently needed. Activin A, member the transforming growth factor β (TGFβ) superfamily, has been suggested to stimulate proliferation invasion osteosarcoma cells in vitro, thus representing potential therapeutic target. In this study, inhibition activin receptor signaling pathway was explored as therapy for osteosarcoma. murine intratibial xenograft model, two types...
Abstract Breast cancer progression to visceral organs such as lung and liver is regarded a dreadful event, unequivocally associated with poor prognosis. Yet, these vital sites are characterized by highly diverse cellular microenvironments physiological functions, suggesting that they may influence cells behavior in divergent ways. Unexpectedly, we find while the microenvironment fosters metastasis-boosting properties of its metastatic seeds favors secondary spread, lungs impose drastic...
Introduction. According to clinical and epidemiological studies, ovarian cancer ranks fifth in deaths among women. The causes of remain largely unknown but various factors may increase the risk developing it, such as age, family history cancer, childbearing status etc. This results from a succession genetic alterations involving oncogenes tumour suppressor genes, which have critical role normal cell growth regulation. Mutations and/or overexpression three oncogenes, c-erbB-2, c-Myc K-ras,...
Summary DNA repair is directly performed by hundreds of core factors and indirectly regulated thousands others. We massively expanded a CRISPR inhibition Cas9-editing screening system to discover modulating homology directed (HDR) in the context ∼18’000 individual gene knockdowns. focused on CCAR1, poorly understood that we found reduced both HDR interstrand crosslink repair, phenocopying loss Fanconi Anemia pathway. CCAR1 abrogated FANCA protein without substantial reduction level its mRNA...