A5023753470- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- RNA modifications and cancer
- Protein Tyrosine Phosphatases
- Chemokine receptors and signaling
- Cancer Cells and Metastasis
- Immune Cell Function and Interaction
- Cancer, Hypoxia, and Metabolism
- Ferroptosis and cancer prognosis
- interferon and immune responses
- Immune cells in cancer
- Cancer-related Molecular Pathways
- Protein Degradation and Inhibitors
- Cancer Research and Treatments
- Mathematical Biology Tumor Growth
- T-cell and B-cell Immunology
- Cytokine Signaling Pathways and Interactions
- Cancer Genomics and Diagnostics
- Pancreatic and Hepatic Oncology Research
- Mesenchymal stem cell research
- Galectins and Cancer Biology
- Cell death mechanisms and regulation
- Systemic Sclerosis and Related Diseases
- Monoclonal and Polyclonal Antibodies Research
- Neuroblastoma Research and Treatments
University of Palermo
2014-2021
After tissue damage, inflammatory cells infiltrate the and release proinflammatory cytokines. HMGB1 (high mobility group box 1), a nuclear protein released by necrotic severely stressed cells, promotes cytokine via its interaction with TLR4 (Toll-like receptor 4) cell migration an unknown mechanism. We show that HMGB1-induced recruitment of depends on CXCL12. CXCL12 form heterocomplex, which we characterized magnetic resonance surface plasmon resonance, acts exclusively through CXCR4 not...
Background Leukocyte migration is essential for effective host defense against invading pathogens and during immune homeostasis. A hallmark of the regulation this process presentation chemokines in gradients stimulating leukocyte chemotaxis via cognate chemokine receptors. For efficient migration, receptor responsiveness must be maintained whilst cells crawl on cell surfaces or matrices along attracting gradient towards increasing concentrations agonist. On other hand agonist-induced...
Current combined surgical and neo-adjuvant chemotherapy of primary metastatic osteosarcoma (OS) is ineffective, reflected by a 5-year survival rate affected patients less than 20 %. Studies in experimental OS metastasis models pointed to the CXCR4/CXCL12 homing axis as novel target for metastasis-suppressive treatment. The present study investigated first time CXCR4-blocking principle spontaneously metastasizing human 143B cell line-derived orthotopic xenograft mouse model. highly cells,...
It has been proposed that mesenchymal stromal cells (MSCs) promote tumor progression by interacting with and other stroma in the complex network of microenvironment. We characterized MSCs isolated expanded from tissues pediatric patients diagnosed neuroblastomas (NB-MSCs) to define interactions microenvironment.Specimens were obtained 7 neuroblastoma (NB). Morphology, immunophenotype, differentiation capacity, proliferative growth, expression stemness neural markers evaluated. Moreover,...
Abstract The tumor microenvironment supplies proinflammatory cytokines favoring a permissive milieu for cancer cell growth and invasive behavior. Here we show how breast progression is facilitated by IL4 secreted adipose tissue estrogen receptor–positive triple-negative types. Blocking autocrine paracrine signaling with the IL4Rα antagonist IL4DM compromised proliferation, invasion, downregulating MAPK pathway activity. reduced numbers of CD44+/CD24− stem-like cells elevated expression dual...
The use of stem cells, including mesenchymal cells (MSCs), for regenerative medicine is gaining interest the clinical benefits so far obtained in patients. This study investigates adipose autologous tissue combination with platelet-rich plasma (PRP) to improve outcome patients affected by systemic sclerosis (SSc). Adipose-derived (AD-MSCs) and PRPs were purified from healthy donors SSc multilineage differentiation potential AD-MSCs their genotypic–phenotypic features investigated. A cytokine...
Immune checkpoint blockade therapy has changed prognoses for many melanoma patients. However, immune responses that correlate with clinical progression of the disease are still poorly understood. To identify correlating evolution, we analyzed serum cytokines as well circulating NK and T-cell subpopulations from The patients' profiles suggested leads to changes in peripheral blood subsets. Stage IV was characterized by an increased frequency CCR7+CD56bright cells high concentrations CCR7...
Abstract Despite intense research and clinical efforts, patients affected by advanced colorectal cancer (CRC) have still a poor prognosis. The discovery of (CR) stem cell (CSC) as the compartment responsible for tumor initiation propagation may provide new opportunities development therapeutic strategies. Given reduced sensitivity CR-CSCs to chemotherapy ability bone morphogenetic proteins (BMP) promote colonic differentiation, we aimed investigate whether an enhanced variant BMP7 (BMP7v)...
P63 is a transcription factor belonging to the family of p53, essential for development and differentiation epithelia. In recent years, it has become clear that altered expression different isoforms this gene can play an important role in carcinogenesis. The p63 encodes two main known as TA ΔN with functions. these sustaining tumor progression metastatic spreading however not entirely been clarified. Here we show breast cancer initiating cells express ΔNp63 isoform supports more mesenchymal...
Background Cancer stem cells represent a population of immature tumor found in most solid tumors. Their peculiar features make them ideal models for studying drug resistance and sensitivity. In this study, we investigated whether cancer isolation vitro sensitivity assay are feasible clinical setting. Methods were isolated from effusions or fresh tissue 23 patients who progressed after standard therapy failure. Specific culture conditions selected that express markers stemness. These exposed...
Chemokine receptors are key regulators of leukocyte trafficking but also have an important role in development, tumor growth, and metastasis. Among the chemokine receptors, CXCR4 is only one that leads to perinatal death when genetically ablated mice, indicating a more-widespread function development. To identify pathways activated downstream CXCR4, solubilization protocol was elaborated, which allows for isolation endogenous receptor from human cells its near-native conformation....
Abstract Background: Despite the advent of successful treatment localized malignancies, metastatic cancer still lacks efficacious therapeutic approaches, including breast cancer. It is now well established that within malignant tumors Cancer Stem Cells (CSCs) constitute a unique cell subset fuel and succeed at tumor growth metastases formation. Tumor microenvironment sustains CSCs characteristics, making molecular mechanisms driving progression recurrence more complex difficult to elucidate....
<div>Abstract<p>The tumor microenvironment supplies proinflammatory cytokines favoring a permissive milieu for cancer cell growth and invasive behavior. Here we show how breast progression is facilitated by IL4 secreted adipose tissue estrogen receptor–positive triple-negative types. Blocking autocrine paracrine signaling with the IL4Rα antagonist IL4DM compromised proliferation, invasion, downregulating MAPK pathway activity. reduced numbers of...
<p>IL-4DM up-regulates DUSP4 expression</p>
<p>DUSP4 reduces aggressiveness of ER+ and TN cancer cells</p>
<p>IL-4 promotes the proliferation of breast cancer cells</p>
<p>IL-4DM blocks IL-4 pathway activation in breast cancer cells</p>
<p>Supplementary Material and Methods Supplementary Figure Legends</p>
<p>IL-4 contributes to the pro-tumorigenic breast cancer milieu</p>
<p>DUSP4 reduces aggressiveness of ER+ and TN cancer cells</p>
<p>Supplementary Material and Methods Supplementary Figure Legends</p>
<p>IL-4 contributes to the pro-tumorigenic breast cancer milieu</p>