A5041560313- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- CAR-T cell therapy research
- Tuberculosis Research and Epidemiology
- RNA modifications and cancer
- Protein Tyrosine Phosphatases
- Multiple Myeloma Research and Treatments
- Mycobacterium research and diagnosis
- Monoclonal and Polyclonal Antibodies Research
- Immune Response and Inflammation
- Immunodeficiency and Autoimmune Disorders
- Mesenchymal stem cell research
- Dietary Effects on Health
- Ferroptosis and cancer prognosis
- Genetics, Aging, and Longevity in Model Organisms
- Cancer, Hypoxia, and Metabolism
- interferon and immune responses
- Hematological disorders and diagnostics
- Chemokine receptors and signaling
- Cancer Cells and Metastasis
- Inflammatory Bowel Disease
- Protein Degradation and Inhibitors
- Atherosclerosis and Cardiovascular Diseases
University of Palermo
2016-2025
Azienda Ospedaliera Universitaria Policlinico "Paolo Giaccone" di Palermo
2022-2024
Erasmus MC Cancer Institute
2023
Tecnologie Avanzate (Italy)
2004-2011
The Ohio State University
2003
Abstract The increasing evidence that γδ T cells have potent antitumor activity suggests their value in immunotherapy, particularly areas of unmet need such as metastatic carcinoma. To this end, we initiated a phase I clinical trial hormone-refractory prostate cancer to examine the feasibility and consequences using T-cell agonist zoledronate, either alone or combination with low-dose interleukin 2 (IL-2), activate peripheral blood cells. Nine patients were enlisted each arm. Neither...
Summary The potent anti-tumour activities of γδ T cells have prompted the development protocols in which γδ-agonists are administered to cancer patients. Encouraging results from small Phase I trials fuelled efforts characterize more clearly application this approach unmet clinical needs such as metastatic carcinoma. To examine breast cancer, a trial was conducted zoledronate, Vγ9Vδ2 cell agonist, plus low-dose interleukin (IL)-2 were 10 therapeutically terminal, advanced Treatment well...
γδ T lymphocytes represent ∼1% of human peripheral blood mononuclear cells and even more in most tissues vertebrates. Although they have important anticancer functions, current single-cell RNA sequencing (scRNA-seq) studies do not identify because their transcriptomes at the level are unknown. Here we show that high-resolution clustering large scRNA-seq datasets a combination gene signatures allow specific detection identification cell receptor (TCR)Vδ1 TCRVδ2 subsets from complex mixtures....
Abstract Colon cancer comprises a small population of stem cells (CSC) that is responsible for tumor maintenance and resistant to therapies, possibly allowing recapitulation once treatment stops. We previously demonstrated such chemoresistance mediated by autocrine production IL-4 through the up-regulation antiapoptotic proteins. Several innate adaptive immune effector allow recognition destruction precursors before they constitute mass. However, cellular immune-based therapies have not been...
Abstract Our study is designed to assess if exosomes released from chronic myelogenous leukemia (CML) cells may modulate angiogenesis. We have isolated and characterized the generated LAMA84 CML demonstrated that addition of human vascular endothelial (HUVEC) induces an increase both ICAM‐1 VCAM‐1 cell adhesion molecules interleukin‐8 expression. The stimulation cell‐cell was paralleled by a dose‐dependent HUVEC monolayer. further showed treatment with caused in motility accompanied loss...
Abstract Imatinib mesylate (imatinib), a competitive inhibitor of the BCR-ABL tyrosine kinase, is highly effective against chronic myelogenous leukemia (CML) cells. However, because 20–30% patients affected by CML display either primary or secondary resistance to imatinib, intentional activation Vγ9Vδ2 T cells phosphoantigens agents that cause their accumulation within cells, such as zoledronate, may represent promising strategy for design novel and innovative immunotherapy capable overcome...
γδ T cells usually infiltrate many different types of cancer, but it is unclear whether they inhibit or promote tumor progression. Moreover, properties tumor-infiltrating and those in the corresponding normal tissue remain largely unknown. Here we have studied features colorectal colon peripheral blood, correlated their levels with clinicopathologic hallmarks. Flow cytometry transcriptome analyses showed that comprised a highly variable rate TILs (5–90%) 4% on average, majority expressing...
T lymphocytes are often induced naturally in melanoma patients and infiltrate tumors. Given that γδ cells mediate antigen-specific killing of tumor cells, we studied the representation vitro cytokine production cytotoxic activity infiltrating from 74 with primary melanoma. We found represent major lymphocyte population melanoma, both Vδ1+ Vδ2+ involved. The majority melanoma-infiltrating showed effector memory terminally-differentiated phenotypes and, accordingly, polyclonal cell lines...
γδ T cells account for a large fraction of human intestinal intraepithelial lymphocytes (IELs) endowed with potent antitumor activities. However, little is known about their origin, phenotype, and clinical relevance in colorectal cancer (CRC). To determine IEL gut specificity, homing, functions, were purified from healthy blood, lymph nodes, liver, skin, intestine, either disease-free, affected by CRC, or generated thymic precursors. The constitutive expression NKp46 specifically identifies...
Chronic inflammation is associated with the occurrence of several diseases. However, side effects anti-inflammatory drugs prompt identification new therapeutic strategies. Plant-derived extracellular vesicles (PDEVs) are gaining increasing interest in scientific community for their biological properties. We isolated PDEVs from juice Citrus limon L. (LEVs) and characterized flavonoid, limonoid lipid contents through reversed-phase high-performance liquid chromatography coupled to electrospray...
Abstract Vγ9Vδ2 T lymphocytes recognize nonpeptidic Ags and mount effector functions in cellular immune responses against microorganisms tumors, but little is known about their role Ab-mediated responses. We show here that expression of CXCR5 identifies a unique subset cells which express the costimulatory molecules ICOS CD40L, secrete IL-2, IL-4, IL-10 help B for Ab production. These properties portray CXCR5+Vγ9Vδ2 as distinct memory cell with helper function.
D6 is a decoy and scavenger receptor for inflammatory CC chemokines. D6-deficient mice were rapidly killed by intranasal administration of low doses Mycobacterium tuberculosis. The death D6(-/-) was associated with dramatic local systemic response levels M. tuberculosis colony-forming units similar to control D6-proficient mice. showed an increased numbers mononuclear cells (macrophages, dendritic cells, CD4 CD8 T lymphocytes) infiltrating inflamed tissues lymph nodes, as well abnormal...
CD8 T-cells contribute to control of Mycobacterium tuberculosis infection, but little is known about the quality T-cell response in subjects with latent infection and patients active disease. recognizing epitopes from 6 different proteins were detected by tetramer staining. Intracellular cytokines staining for specific production IFN-γ IL-2 was performed, complemented phenotyping memory markers on antigen-specific T-cells. The ex-vivo frequencies tetramer-specific tuberculous before therapy...
The identification of reciprocal interactions between tumor-infiltrating immune cells and the microenviroment may help us understand mechanisms tumor growth inhibition or progression. We have assessed frequencies circulating γδ T regulatory (Treg) from 47 patients with squamous cell carcinoma (SCC), to determine if they correlated progression survival. Vδ1 infiltrated SSC tissue a greater extent than normal skin, but SCC healthy subjects had similar amounts circulating. However, Vδ2 were...
Abstract The immunophenotype of oldest centenarians, i.e. semi- and supercentenarians, could provide important information about their ability to adapt factors associated with immune changes, including ageing per se chronic Cytomegalovirus infection. We investigated, by flow cytometry, variations in percentages absolute numbers cell subsets, focusing on T cells, pro-inflammatory parameters a cohort 28 women 26 men (age range 19–110 years). observed variability hallmarks immunosenescence...
Abstract Melanoma is one of the most sensitive tumors to immune modulation, and major challenge for melanoma patients’ survival checkpoint inhibitor (ICI) therapy. γδ T lymphocytes play an antitumoral role in a broad variety including they are optimal candidates cellular immunotherapy. Thus, comprehensive analysis correlation between cells receptors context was conducted, with aim devising innovative combined immunotherapeutic strategy. In this study, using GEPIA2.0 database, significant...
We have compared four human subsets of Vγ9Vδ2 T cells, naive (Tnaive, CD45RA+CD27+), central memory (TCM, CD45RA–CD27+), effector (TEM, CD45RA–CD27–) and terminally differentiated (TEMRA, CD45RA+CD27–), for their capacity to proliferate differentiate in response antigen or homeostatic cytokines. Cytokine responsiveness IL-15R expression were low Tnaive cells progressively increased from TCM TEM TEMRA cells. In contrast, the expand cytokine stimulation showed a reciprocal pattern was...