A5075263979- Spondyloarthritis Studies and Treatments
- Autoimmune and Inflammatory Disorders Research
- Rheumatoid Arthritis Research and Therapies
- Psoriasis: Treatment and Pathogenesis
- Salivary Gland Disorders and Functions
- Immune Cell Function and Interaction
- Systemic Sclerosis and Related Diseases
- Systemic Lupus Erythematosus Research
- IL-33, ST2, and ILC Pathways
- T-cell and B-cell Immunology
- Vasculitis and related conditions
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Tuberculosis Research and Epidemiology
- Inflammasome and immune disorders
- Atherosclerosis and Cardiovascular Diseases
- Dermatology and Skin Diseases
- Urticaria and Related Conditions
- Inflammatory Myopathies and Dermatomyositis
- Eosinophilic Esophagitis
- Kawasaki Disease and Coronary Complications
- Cytokine Signaling Pathways and Interactions
- Immunotherapy and Immune Responses
- Dermatologic Treatments and Research
- Cell Adhesion Molecules Research
- Salivary Gland Tumors Diagnosis and Treatment
University of Palermo
2016-2025
Azienda Ospedaliera Universitaria Policlinico "Paolo Giaccone" di Palermo
2019-2023
ORCID
2021
University of Campania "Luigi Vanvitelli"
2019
Ospedale Vincenzo Cervello
2012-2018
Toronto Western Hospital
2018
University Health Network
2018
University of Palermo
2013-2017
University of L'Aquila
2016
Amsterdam UMC Location University of Amsterdam
2016
Th1 CD4(+) T cells and their derived cytokines are crucial for protection against Mycobacterium tuberculosis. Using multiparametric flow cytometry, we have evaluated the distribution of seven distinct functional states (IFN-γ/IL-2/TNF-α triple expressors, IFN-γ/IL-2, IFN-γ/TNF-α or TNF-α/IL-2 double expressors IFN-γ, IL-2 TNF-α single expressors) in individuals with latent M. tuberculosis infection (LTBI) active (TB). We found that while detectable 85-90%TB patients, were only present 10-15%...
Summary The potent anti-tumour activities of γδ T cells have prompted the development protocols in which γδ-agonists are administered to cancer patients. Encouraging results from small Phase I trials fuelled efforts characterize more clearly application this approach unmet clinical needs such as metastatic carcinoma. To examine breast cancer, a trial was conducted zoledronate, Vγ9Vδ2 cell agonist, plus low-dose interleukin (IL)-2 were 10 therapeutically terminal, advanced Treatment well...
The aim of the study was to better characterise immunological origin and behaviour interleukin (IL)-23-responsive innate lymphoid cells (ILCs) in gut, synovial fluid (SF) bone marrow (BM) patients with ankylosing spondylitis (AS).ILC1, ILC2 ILC3 were determined characterised by confocal microscopy flow cytometry ileal BM biopsies, peripheral blood (PB) SF mononuclear obtained from AS controls. Mucosal vascular addressin cell adhesion molecule 1 (MADCAM-1), IL-7, IL-15 aggregates tissue...
Dysbiosis has been recently demonstrated in patients with ankylosing spondylitis (AS) but its implications the modulation of intestinal immune responses have never studied. The aim this study was to investigate role ileal bacteria modulating local and systemic AS.Ileal biopsies were obtained from 50 HLA-B27+ AS 20 normal subjects. Silver stain used visualise bacteria. Ileal expression tight adherens junction proteins investigated by TaqMan real-time (RT)-PCR immunohistochemistry. Serum...
<h3>Objectives</h3> Interleukin (IL)-23 has been implicated in the pathogenesis of ankylosing spondylitis (AS). The aim study was to clarify mechanisms underlying increased IL-23 expression gut AS patients. <h3>Methods</h3> Consecutive biopsies from 30 HLA-B27<sup>+</sup> patients, 15 Crohn9s disease (CD) patients and 10 normal subjects were obtained. Evidence for HLA-B27 misfolding studied. Unfolded protein response (UPR) autophagy assessed by RT-PCR immunohistochemistry. contribution UPR...
In chronic inflammatory disorders, interleukin (IL)-22 may act either as a protective or pro-inflammatory cytokine. At mucosal sites, IL-22 is mainly produced by CD4(+) T cells and subset of natural killer (NK) expressing the receptor NKp44 (NKp44(+) NK cells). The aim this study was to investigate expression in salivary glands patients with primary Sjögren's syndrome (pSS).Minor gland biopsies were obtained from 19 pSS 16 non-specific sialoadenitis. Quantitative gene analysis TaqMan...
Adult-onset Still’s disease (AOSD) is rare inflammatory of unknown etiology that usually affects young adults. The more common clinical manifestations are spiking fevers, arthritis, evanescent rash, elevated liver enzymes, lymphadenopathy, hepatosplenomegaly, and serositis. multi-visceral involvement the different complications, such as macrophage activation syndrome, may strongly decrease life expectancy AOSD patients. This study aimed to identify positive negative features correlated with...
Abstract Imatinib mesylate (imatinib), a competitive inhibitor of the BCR-ABL tyrosine kinase, is highly effective against chronic myelogenous leukemia (CML) cells. However, because 20–30% patients affected by CML display either primary or secondary resistance to imatinib, intentional activation Vγ9Vδ2 T cells phosphoantigens agents that cause their accumulation within cells, such as zoledronate, may represent promising strategy for design novel and innovative immunotherapy capable overcome...
Objective. IL-9 has been shown to be upregulated before the clinical onset of articular disease in RA. The exact role and Th9 cells RA, however, not yet adequately studied. aim this study was evaluate expression IL-9-expressing RA patients.
The aim of this study was to investigate the expression interleukin (IL)-36 axis in patients with primary Sjögren's syndrome (pSS). Blood and minor labial salivary glands (MSG) biopsies were obtained from 35 pSS 20 non-Sjögren's (nSS) patients. Serum IL-36α assayed by enzyme-linked immunosorbent assay (ELISA). IL-36α, IL-36R, IL-36RA, IL-38, IL-22, IL-17, IL-23p19 MSGs assessed reverse transcription-polymerase chain reaction (RT-PCR), tissue IL-38 also investigated immunohistochemistry...
Objectives. To investigate the expression of IL-34 in labial salivary glands (LSGs) patients with primary SS (p-SS) and its role inducing a pro-inflammatory monocyte phenotype. Methods. LSG biopsies were obtained from 20 p-SS 10 non-Sjögren's sicca syndrome (n-SS). The IL-34, IL-1β, TNF-α, IL-17 IL-23 was assessed by real-time PCR. also investigated LSGs immunohistochemistry. frequencies subpopulations CD14+ monocytes evaluated flow cytometry among isolated mononuclear cells peripheral blood...
CD8 T-cells contribute to control of Mycobacterium tuberculosis infection, but little is known about the quality T-cell response in subjects with latent infection and patients active disease. recognizing epitopes from 6 different proteins were detected by tetramer staining. Intracellular cytokines staining for specific production IFN-γ IL-2 was performed, complemented phenotyping memory markers on antigen-specific T-cells. The ex-vivo frequencies tetramer-specific tuberculous before therapy...
Objective To investigate the expression and tissue distribution of Th9‐related cytokines in patients with psoriatic arthritis (PsA). Methods Quantitative gene analysis Th1, Th17, Th9 was performed intestinal biopsy samples obtained from PsA, HLA−B27−positive ankylosing spondylitis (AS), Crohn's disease (CD), healthy controls. Expression interleukin‐23 (IL‐23), IL‐17, IL‐22, IL‐9, IL‐9 receptor (IL‐9R) were evaluated by immunohistochemistry confocal microscopy. Flow cytometry used to study...
CD4+ CD28− T cells also called CD28 null have been reported as increased in the clinical setting of acute coronary syndrome. Only 2 studies previously analyzed peripheral frequency subjects with ischemic stroke but, to our knowledge, each TOAST subtype has never evaluated. We hypothesized that and, particular, cell subset could show a different degree percentage relation and severity stroke. The aim study was analyze diagnostic subtype, evaluate their relationship scores stroke, predictive...
Immunosuppressive drugs in clinical transplantation are necessary to inhibit the immune response donor antigens. Although they effective controlling acute rejection, do not prevent long-term transplant loss from chronic rejection. In addition, immunosuppressive have adverse side effects, including increased rate of infections and malignancies. Adoptive cell therapy with human Tregs represents a promising strategy for induction tolerance. Phase I/II trials transplanted patients already...
Objective. GCA is a large- and medium-vessel arteritis characterized by range of histological patterns vascular wall injury. The aim this study was to immunologically characterize the various GCA. Methods. Thirty-five consecutive patients with biopsy-proven 15 normal controls were studied. IL-8, IL-9, IL-9R, IL-17, IL-4, TGF-β thymic stromal lymphopoietin expression evaluated RT–PCR immunohistochemistry on artery biopsy specimens. Confocal microscopy used phenotypes IL-9-producing...
Objectives To investigate whether artery tertiary lymphoid organs (ATLOs) are present in giant cell arteritis (GCA) and that their formation is associated with the ectopic expression of constitutive tissue-homing chemokines. Methods Reverse transcriptase PCR, immunohistochemical immunofluorescence analysis were used to determine presence ATLOs GCA chemokines/chemokine receptors cytokines involved lymphoneogenesis temporal samples obtained from 50 patients 30 controls. The lymphatic conduits,...
To characterize the ecological effects of biologic therapies on gut bacterial and fungal microbiome in psoriatic arthritis (PsA)/spondyloarthritis (SpA) patients.Fecal samples from PsA/SpA patients pre- posttreatment with tumor necrosis factor inhibitors (TNFi; n = 15) or an anti-interleukin-17A monoclonal antibody inhibitor (IL-17i; 14) underwent sequencing (16S ribosomal RNA, internal transcribed spacer shotgun metagenomics) computational analysis. Fecal levels fatty acid metabolites...