A5022271744- Asthma and respiratory diseases
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immune Response and Inflammation
- IL-33, ST2, and ILC Pathways
- Immunotherapy and Immune Responses
- Mycobacterium research and diagnosis
- Pediatric health and respiratory diseases
- Tuberculosis Research and Epidemiology
- Fatty Acid Research and Health
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Allergic Rhinitis and Sensitization
- Nicotinic Acetylcholine Receptors Study
- NF-κB Signaling Pathways
- Air Quality and Health Impacts
- Cancer-related molecular mechanisms research
- Inflammatory mediators and NSAID effects
- Cancer, Stress, Anesthesia, and Immune Response
- Proteoglycans and glycosaminoglycans research
- Developmental Biology and Gene Regulation
- Advanced Nanomaterials in Catalysis
- Synthesis and Characterization of Heterocyclic Compounds
- Eosinophilic Esophagitis
- Epigenetics and DNA Methylation
- Cell death mechanisms and regulation
Istituto di Farmacologia Traslazionale
2022-2025
Istituto di Biomedicina e di Immunologia Molecolare Alberto Monroy
2010-2024
National Research Council
2014-2024
Institute for Biomedical Research and Innovation
2019-2022
National Academies of Sciences, Engineering, and Medicine
2011-2014
University of Palermo
1999-2004
The Ohio State University
2003
University of Palermo
2001-2002
Vδ2 T lymphocytes recognize nonpeptidic antigens without presentation by MHC molecules and mount both immediate effector functions memory responses after microbial infection. However, how cells mediate different facets of a response remains unknown. Here, we show that the expression CD45RA CD27 defines four subsets human with distinctive compartmentalization routes. Naive CD45RA+CD27+ CD45RA−CD27+ express lymph node homing receptors, abound in nodes, lack functions. Conversely, CD45RA−CD27−...
The beneficial health properties of the Mediterranean diet are well recognized. principle source fat in Mediterranean is extra-virgin olive oil (EVOO). Oleocanthal (OC) a naturally occurring minor phenolic compound isolated from EVOO, which has shown potent anti-inflammatory activity, by means its ability to inhibit cyclooxygenase (COX) enzymes COX-1 and COX-2. A large body evidence indicates that phenols exhibit anticancer activities. aim present study was evaluate potential effects OC...
Extracellular vesicles (EVs) are membrane-enclosed bio-nanoparticles secreted by cells and naturally evolved to transport various bioactive molecules between even organisms. These cellular objects considered one of the most promising bio-nanovehicles for delivery native exogenous molecular cargo. However, many challenges with state-of-the-art EV-based candidates as drug carriers still exist, including issues scalability, batch-to-batch reproducibility, cost-sustainability final therapeutic...
Abstract The lungs are considered to have an impaired capacity contain infection by pathogenic mycobacteria, even in the presence of effective systemic immunity. In attempt understand underlying cellular mechanisms, we characterized γδ T cell population following intranasal with Mycobacterium bovis bacillus Calmette-Guérin (BCG). peak expansion at 7 days postinfection preceded 30 day αβ and bacterial count. expanded cells BCG-infected mice represents resident Vγ2 subset as well influx Vγ1...
Citrus IntegroPectin bioconjugates obtained through acoustic cavitation in water of different citrus fruit (lemon, red orange, and sweet orange) processing waste show substantial anticancer activity vitro against human non-small cell lung cancer cells. Dis-solved aqueous phase at concentrations, all phytocomplexes tested affected long-term proliferation migration adeno-carcinoma cells line A549. Compared to from lemon orange were more effective reducing colony formation activity. Pointing...
IL-17A is involved in the activation of oxidative stress and inflammation nasal epithelial cells. Hyaluronan (HA) its high molecular weight form (HMW-HA) shows anti-inflammatory responses contrast to low medium HA (LMW-HA MMW-HA). The aim this study was investigate pro- or biologic function at different an vitro model mediated. We evaluated ERK1/2 IκBα phosphorylation, NF-κB signal pathway activation, ROS production, IL-8 NOX-4 protein, mRNA levels, cells RPMI 2650 stimulated with...
Abstract Hepatocellular carcinoma (HCC) is one of the common malignancies and an increasingly important cause cancer death worldwide. Surgery, chemotherapy, radiation therapy extend 5-year survival limit in HCC patients by only 6%. Therefore, there a need to develop new therapeutic approaches for treatment this disease. The orally bioavailable proteasome inhibitor MLN2238 (ixazomib) has been demonstrated have anticancer activity. In present study, we investigated preclinical efficacy cells...
Abstract The possible contribution of NKT cells to resistance Mycobacterium tuberculosis infection remains unclear. In this paper we characterized the Vα14 cell population following with bovis bacillus Calmette-Guérin (BCG). BCG determined an early expansion in liver, lungs, and spleen, which peaked on day 8 was sustained until 30. However, NK1.1+ preferentially producing IFN-γ predominated at stage (day 8), substituted by NK1.1− IL-4 later stages 30). Despite fact that cell-deficient mice...
Th17 cells and IL-17A play a role in the development progression of allergic diseases. We analyzed levels sputum supernatants (Ss), nasal wash (NW) plasma (P) from Healthy Controls (HC) children with Asthma/Rhinitis. tested expression IL-17A, RORγ(t) FOXP3 peripheral blood T-lymphocytes intermittent mild-moderate asthma. The effect Budesonide Formoterol was “in vitro” on cultured asthma/persistent rhinitis patients, bronchial epithelial stimulated NW Ss rhinitis. Further, 12 weeks treatment...
In Italy, the nsLTP (Pru p 3) has been identified as most frequent cause of food allergy and anaphylaxis. order to estimate risk assessment in peach allergy, we investigated presence correlations between levels sIgE Pru 3 with severity clinical symptoms two positive populations from different areas Italy.133 consecutively patients were recruited South where prevalence PR-10 profilin sensitization is low, North-East pathogenesis related protein -10 (PR-10) higher. Skin prick test (SPT)...
The integrity of the respiratory epithelium is crucial for airway homeostasis. Tobacco smoke exposure and recurrent infections airways play a role in progression decline function chronic obstructive pulmonary disease (COPD). aim this study was to detect differentially expressed proteins bronchial epithelial cell line (16-HBE) stimulated with cigarette extract (CSE) lipopolysaccharide (LPS), constituent gram-negative bacteria, alone and/or combination, by using two-dimensional electrophoresis...
Melanoma is the most aggressive form of skin cancer and one treatment-refractory malignancies. In metastatic melanoma cell lines, we analysed anti-proliferative pro-apoptotic potentials a phenolic component olive oil, hydroxytyrosol. particular, through MTS assay, DeadEnd™ Colorimetric TUNEL Annexin V binding PI uptake, western blot experiment, intracellular reactive oxygen species (ROS) analysis, colony showed that hydroxytyrosol treatment remarkably reduces viability inducing death for...
Carotenoids may have different effects on cancer and its progression. The safety of carotenoid supplements was evaluated in vitro human non-small cell lung (NSCLC) adenocarcinoma A549 cells by the administration three oleoresins containing lycopene other lipophilic phytochemicals, such as tocochromanols. oleoresins, obtained supercritical CO2 green extraction technology from watermelon (Lyc W), gấc(Lyc G) tomato T) chlatrated α-cyclodextrins, were tested comparison to synthetic S), cycle,...
The specificity of CD4 T lymphocytes was investigated in 6 patients affected by tuberculosis who had negative tuberculin purified protein derivative (PPD) skin tests at diagnosis. Polyclonal cell lines from the peripheral blood failed to proliferate PPD and 16-or 38-kDa proteins Mycobacterium tuberculosis, while disease site responded 16- derived epitopes vitro. Four months after chemotherapy, became responsive PPD. proliferative response or their peptides decreased increased blood. These...
Human T cells expressing Vγ9/Vδ2-encoded TCR recognize several nonpeptide phosphoantigens in the absence of major histocompatibility complex restriction. As these respond differentially to increasing concentrations structurally related phosphoantigens, such ligands constitute agonists different strengths. By analyzing early cellular events and late effector responses γ δ cells, we compared their patterns stimulation by weak, medium strong phosphoantigen agonists. We found that, although...
CD8(+) T cells could make an important contribution to protection against tuberculosis (TB), but the antigenic determinants recognized in context of major histocompatibility complex class I molecules remain ill defined. Our aim was identify nonamer peptides derived from acr/16-kDa antigen. Two immunogenic (p21-29 and p120-128) were identified by their ability elicit cytotoxic juvenile patients recovering TB. Epitope-specific recognition demonstrated lysis both Mycobacterium...
Vgamma9/Vdelta2 T cells can contribute to protective immune response against Mycobacterium tuberculosis, although the extent which and mechanisms by they could actually protect human tuberculosis remain unclear. We have previously reported that from tuberculin purified protein derivative (PPD)-positive children, either healthy or affected different clinical forms of strongly proliferate phosphoantigens in vitro, whereas PPD-negative subjects very poorly. report here tuberculous children an...
TGF-β-targeting structural and inflammatory cells has been implicated in the mechanisms leading to restructuring processes asthma, suggesting an impact of TGF-β1 signaling on development persistency this disease. We investigated potential early involvement activity immunological molecular underlying progression inflammation childhood asthma. evaluated levels induced sputum supernatants (ISSs) expression small mother cell against decapentaplegic (Smad) 2 Smad7 proteins (ISCs) from children...