A5042874918- Tuberculosis Research and Epidemiology
- Heat shock proteins research
- Mycobacterium research and diagnosis
- Immune Cell Function and Interaction
- Immune Response and Inflammation
- Immunotherapy and Immune Responses
- HIV Research and Treatment
- T-cell and B-cell Immunology
- Biochemical and Molecular Research
- Enzyme Structure and Function
- Atherosclerosis and Cardiovascular Diseases
- Pharmacological Effects of Natural Compounds
- Legume Nitrogen Fixing Symbiosis
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- RNA and protein synthesis mechanisms
- Escherichia coli research studies
- Bacterial Genetics and Biotechnology
- Endoplasmic Reticulum Stress and Disease
- Monoclonal and Polyclonal Antibodies Research
- Bacteriophages and microbial interactions
- Yersinia bacterium, plague, ectoparasites research
- Vector-borne infectious diseases
- Diagnosis and treatment of tuberculosis
- Viral gastroenteritis research and epidemiology
- Exercise and Physiological Responses
Pandit Bhagwat Dayal Sharma University of Health Sciences
2025
Lionex (Germany)
2013-2023
University of California, Los Angeles
2007-2023
Pandit Bhagwat Dayal Sharma Post Graduate Institute of Medical Sciences
2003-2023
Himachal Pradesh University
2022
Indian Institute of Management Rohtak
2018
Helmholtz Centre for Infection Research
2000-2015
Institute of Medical Sciences
2012-2015
ESI - Post Graduate Institute of Medical Science and Research
2015
Shoolini University
2013
Abstract The peptide binding C-terminal portion of heat shock protein (HSP)70 (aa 359–610) stimulates human monocytes to produce IL-12, TNF-α, NO, and C-C chemokines. N-terminal, ATPase (HSP701–358) failed stimulate any these cytokines or Both native the truncated HSP70359–610 stimulation chemokine production is mediated by CD40 costimulatory molecule. Maturation dendritic cells was induced with HSP70, not seen N-terminal HSP701–358, but enhanced HSP70359–610, as demonstrated up-regulation...
Abstract Immunization with Mycobacterium tuberculosis heat shock protein (hsp) 60 has been shown to protect rats from experimental arthritis. Previously, the protection-inducing capacity was reside in evolutionary conserved parts of molecule. Now we have studied nature arthritis suppressive a distinct, antigenically unrelated protein, M. hsp70. Again, mycobacterial hsp70 sequence found be immunogenic and induce T cells that cross-reacted rat homologue sequence. However, this case parenteral...
Although the Toll-like receptors used by Mycobacterium tuberculosis membrane and secreted factors are known, pathways activated M. heat shock proteins not. An efficient immune response against intracellular pathogen is critically dependent on rapid detection of invading innate system coordinated activation adaptive response. Macrophage phagocytosis accompanied transcription factor NF-kappaB secretion inflammatory mediators that play an important role in granuloma formation protection during...
Mutants of Azospirillum brasilense Sp Cd, resistant to 5-fluorotryptophan (FT) excreted 3-indoleacetic acid (IAA), i.e., auxin, producing up 16 μg/mL which was 30 times greater than the wild-type level. Under conditions nitrogen fixation, mutants IAA 1 μg/mL, 10 more wild type. However, none FT-resistant lipoferum RG 20a high levels IAA. This probably due differences in tryptophan and biosynthetic steps between A. strains. Some Cd showed a reduced feedback inhibition anthranilate synthetase...
An effective host immune response to mycobacterial infection must control pathogen dissemination without inducing immunopathology. Constitutive overexpression of heat shock protein (myHsp70) is associated with impaired bacterial persistence, but the immune-mediated mechanisms are unknown. We found that myHsp70, in addition enhancing antigen delivery human dendritic cells, signaled through CCR5 chemokine receptor, promoting cell aggregation, synapse formation between cells and T generation...
Reestablishing self-tolerance in autoimmunity is thought to depend on self-reactive regulatory T cells (Tregs). Exploiting these antigen-specific regulators hampered by the obscure nature of disease-relevant autoantigens. We have uncovered potent disease-suppressive Tregs recognizing Heat Shock Protein (Hsp) 70 self-antigens, enabling selective activity inflamed tissues. Hsp70 a major contributor MHC class II ligandome. Here we show that conserved epitope (B29) present murine and upon...
Abstract Binding and activation of human plasminogen (Plg) to generate the proteolytic enzyme plasmin (Plm) have been associated with invasive potential certain bacteria. In this work, proteomic analysis together ligand blotting assays identified several major Plg‐binding spots in Mycobacterium tuberculosis soluble extracts (SEs) culture filtrate proteins. The identity 15 different proteins was deduced by N‐terminal and/or MS corresponded DnaK, GroES, GlnA1, Ag85 complex, Mpt51, Mpt64, PrcB,...
Abstract The 70-kDa microbial heat shock protein (mHSP70) has a profound effect on the immune system, interacting with CD40 receptor DC and monocytes to produce cytokines chemokines. mHSP70 also induces maturation of dendritic cells (DC) thus acts as an alternative ligand CD40L T cells. In this investigation, we have identified cytokine-stimulating epitope (peptide 407–426), by activating overlapping synthetic peptides (20-mers) derived from sequence mHSP70. This peptide significantly...
Mycobacterium tuberculosis H37Rv contains the kshA (Rv3526) and kshB (Rv3571) genes, encoding 3-ketosteroid 9alpha-hydroxylase (KSH). Consistent with their predicted roles, DeltakshA DeltakshB deletion mutants of M. were unable to use cholesterol 4-androstene-3,17-dione as primary carbon energy sources. Interestingly, also metabolize steroid substrate 5alpha-androstane-3,17-dione, whereas wild-type could. The either these genes lead rapid death microorganism in murine infection models...
Macrophages and dendritic cells have been recognized as key players in the defense against mycobacterial infection. However, more recently, other lungs such alveolar epithelial (AEC) found to play important roles pathogenesis of In present study we first compared AEC with pulmonary macrophages (PuM) isolated from mice their ability internalize control Bacillus Calmette-Guérin (BCG) growth capacity APCs. were able bacterial well antigen primed T cells. Secondly, both cell types secrete...
ABSTRACT Much attention has focused on the Mycobacterium tuberculosis molecular chaperone chaperonin (Cpn) 60.2 (Hsp 65) in pathology of because its immunogenicity and ability to directly activate human monocytes vascular endothelial cells. However, M . is one a small group bacteria that contain multiple genes encoding Cpn 60 proteins. We have now cloned expressed both proteins report novel 60, 60.1, more potent inducer cytokine synthesis than 60.2. This spite 76% amino acid sequence...
Cysteine biosynthetic genes are up-regulated in the persistent phase of Mycobacterium tuberculosis, and corresponding enzymes therefore interest as potential targets for novel antibacterial agents. cysK1 is one these has been annotated coding an O-acetylserine sulfhydrylase. Recombinant CysK1 a pyridoxal phosphate (PLP)-dependent enzyme that catalyzes conversion to cysteine. The crystal structure was determined 1.8A resolution. belongs family fold type II PLP similar other sulfhydrylases. We...
ABSTRACT We demonstrated that not whole Mycobacterium tuberculosis but its particular antigens, hsp70 Mtb , hsp65 and hsp16 are present in lymph node tissues of patients with sarcoidosis (SA). occurs the early stage SA, whereas II SA. is highly expressed capillary vessels
<b>Background:</b> Acute appendicitis (AA) is the most common surgical emergency in childhood. The risk of rupture negligible within first 24 h, climbing to 6% after 36 h from onset symptoms. Because difficulty accurate diagnosis AA a significant number children still are being managed when it already perforated. There always need make an early and find out factors associated with development complication this condition. <b>Patients Methods:</b> A total 102 patients clinical were admitted...
Tuberculosis (TB) is the leading global cause of death from a single infectious agent. Registered incidence rates are low, especially in low-resource countries with weak health systems, due to disadvantages current diagnostic techniques. A major effort directed develop point-of-care (POC) platform reduce TB deaths prompt and reliable low-cost technique. In frame European POCKET Project, novel POC for direct noninvasive detection human urine was developed. The photonic sensor chip integrated...
We present a low-cost integrated nanophotonic lab-on-a-chip platform suitable for point-of-care (POC) biomarker analysis.
Tuberculosis (TB) is the most deadly infectious disease in existence, and only available vaccine, Bacillus Calmette-Guérin (BCG), almost a century old poorly protective. The immunological complexity of TB, coupled with rising resistance to antimicrobial therapies, necessitates pipeline diverse novel vaccines. Here, we show that B. subtilis spores can be coated fusion protein ('FP1') consisting M. tuberculosis (Mtb) antigens Ag85B, ACR HBHA. resultant Spore-FP1, was tested murine low-dose Mtb...
Abstract Osteitis pubis is a well-known complication of urologic and gynecologic procedures but its association with hernioplasty poorly documented in the literature. We are reporting two cases osteitis following laparoscopic hernia repair. In first case, 52-year-old man underwent bilateral totally extra-peritoneal repair (TEP) for inguinal hernia. Mesh fixation was done metallic (titanium) tackers at iliopubic tract. Two weeks later he complained sharp stabbing pain pubic area dull aching...