A5072190645- Tuberculosis Research and Epidemiology
- Bacteriophages and microbial interactions
- Mycobacterium research and diagnosis
- Genetic and phenotypic traits in livestock
- Brucella: diagnosis, epidemiology, treatment
- Bacterial Genetics and Biotechnology
- Glycosylation and Glycoproteins Research
- Escherichia coli research studies
- Pneumonia and Respiratory Infections
- Salmonella and Campylobacter epidemiology
- Toxoplasma gondii Research Studies
- Parasitic Infections and Diagnostics
- Herpesvirus Infections and Treatments
- Entomopathogenic Microorganisms in Pest Control
- Helminth infection and control
- Cancer therapeutics and mechanisms
- Rabies epidemiology and control
- Pneumocystis jirovecii pneumonia detection and treatment
- Historical Studies on Spain
- Animal Behavior and Welfare Studies
- Business, Innovation, and Economy
- Toxin Mechanisms and Immunotoxins
- Cancer-related gene regulation
- Science, Technology, and Education in Latin America
- Historical Studies in Latin America
Universidad Nacional Autónoma de México
2006-2024
University of Cuenca
2014-2023
Imperial College London
2011
London School of Hygiene & Tropical Medicine
2011
Abstract Binding and activation of human plasminogen (Plg) to generate the proteolytic enzyme plasmin (Plm) have been associated with invasive potential certain bacteria. In this work, proteomic analysis together ligand blotting assays identified several major Plg‐binding spots in Mycobacterium tuberculosis soluble extracts (SEs) culture filtrate proteins. The identity 15 different proteins was deduced by N‐terminal and/or MS corresponded DnaK, GroES, GlnA1, Ag85 complex, Mpt51, Mpt64, PrcB,...
A Mycobacterium tuberculosis culture filtrate enriched with mannose-containing proteins was resolved by 2-DE gel. After ConA ligand blotting, 41 were identified mass spectrometry as putative glycoproteins 34 of them new probably mannosylated proteins. These results contribute to the construction affinity glycoprotein database M. tuberculosis, and provide useful information for understanding biological role in mycobacteria.
ABSTRACT Bovine tuberculosis is a major problem in many countries; hence, new and better diagnostic tools are urgently needed. In this work, we have tested ESAT6, CFP10, PE13, PE5, MPB70, TB10.4, TB27.4 for their potentials as markers field animals from Northern Ireland, Mexico, Argentina, regions with low, medium, high prevalences of bovine tuberculosis, respectively. At all three sites, ESAT6 CFP10 were superior antigens, while combination performed even at the two sites where was tested,...
To identify the resistance phenotype against Mycobacterium bovis in cattle, we used a bactericidal assay that has been considered marker of this trait. Three 24 cows (12.5%) were phenotyped as resistant and 21 susceptible. Resistance bovine macrophages (MΦ) to BCG challenge was evaluated for its association with SLC11A1 GT microsatellite polymorphisms within 3′UTR region. Twenty-three (95.8%) had GT13 genotype, reported resistant, consequently SLC11A1polymorphism not agreement our results....
In this era of increasing drug resistance among infectious diseases such as tuberculosis (TB), the complex population dynamics border areas must be monitored more extensively. TB remains a major public health threat; its antimicrobial treatment is long; and only vaccine licensed in world, live-attenuated Mycobacterium bovis Bacille Calmette-Guérin (BCG), exhibits varying efficacy. addition to epidemiological surveillance, underlying determinants contributing wellbeing populations are key...
Glycosylation is one of the most abundant posttranslational polypeptide chain modification in nature. Although carbohydrate protein antigens from many microbial pathogens constitutes important components B cell epitopes, role T immunity not completely understood. There growing evidence about importance these modifications host bacteria interactions tuberculosis. It known, that sugars present some Mycobacterium tuberculosis glycoproteins play an both humoral and cellular immune response...
Abstract Leucine aminopeptidase (LAP) and cathepsin L1 (CL1) are important enzymes for the pathogenesis physiology of Fasciola hepatica . These were analysed in silico to design a chimeric protein containing most antigenic sequences LAP (GenBank; AAV59016.1; amino acids 192–281) CL1 (GenBank CAC12806.1; 173–309). The cloned 681-bp fragment (rFhLAP-CL1) contains 270 bp from 411 CL1, comprising three epitopes, DGRVVHLKY (amino 54–62) LAP, VTGYYTVHSGSEVELKNLV 119–137) YQSQTCLPF 161–169) CL1....
La Ehrlichiosis monocítica canina (EMC) es causada por Ehrlichia canis, enfermedad transmitida garrapatas de la especie Riphicephalus sanguinius, su presentación clínica puede variar desde fase aguda hasta una crónica, en América Latina existen varios estudios sobre ehrlichiosis donde se considera infección zoonótica emergente humanos. El objetivo esta investigación aplicar prueba PCR convencional (cPCR) muestras caninos seropositivos al test rápido CaniV-4 BioNote ciudad Naranjal...
Abstract Identifying and evaluating potential vaccine candidates has become one of the main objectives to combat tuberculosis. Among them, mannosylated Apa antigen from Mycobacterium tuberculosis non-mannosylated protein expressed in Escherichia coli , have been studied. Although both proteins can induce a protective response mice, it considered that native be dispensed. In this work, we study induced by E. Streptomyces lividans. The latter like is secreted as double band 45/47 kDa, however,...
Esta investigación evaluó el efecto de la proteína recombinante choque térmico HSPA1A en espermatozoides bovinos sometidos a refrigeración durante 96 horas (5 °C), y congelación. Un experimento inicial determinó concentración óptima (0, 15, 30, 45 µg/mL) suplementado al diluyente OPTIXcell, utilizando 18 eyaculados (de 2 toros) refrigerados horas. Posteriormente, se suplementación µg/mL (como óptima) 6 congelación mediante vapores nitrógeno líquido estático. Los resultados indicaron que...
The aim of this study was to analyse the gene and genotypic parameters using microsatellites Creole bovine (CB) from high southern tropics Ecuador contrasting them with registered Holstein (HB); For this, blood collected 7 CC 3 BC subpopulations. Fifteen markers grouped into 4 mixtures were analysed; 1) CSRM60, INRA083, CSSM66; 2) ETH3, HEL9, TGLA53, BM1818, ILSTS006; 3) BM2113, ETH225, TGLA122, ETH10; 4) TGLA227, INRA032, SPS115. Genetic variation determined: number alleles (Na), effective...
En la industria avícola, existen diferentes tipos de Escherichia coli patógenos y no patógenos, según los genes asociados a virulencia (VAG) presentes en bacteria. Sin embargo, reportes sobre identificación VAG E. pollos engorde son limitados, especialmente comunidades andinas ecuatorianas. El objetivo principal este estudio consistió identificar diversidad genética presencia cepas coli. Se caracterizaron varias granjas provincia del Azuay (sur Ecuador) partir 30 aislados cultivos...
La Brucelosis es una enfermedad zoonótica extendida a nivel mundial en el ganado bovino, ocasionada principalmente por Brucella abortus, previamente reportada ciertas regiones del Ecuador. caracterización de las cepas circulantes spp. ganaderías lecheras resulta importante para comprender la epidemiología esta enfermedad. tipificación bacteriológica un proceso lento, riesgoso y requiere laboratorios especializados. El objetivo este estudio fue tipificar que afectan al bovino provincia Azuay,...
In recent years, knowledge of the role that protein methylation is playing on physiopathogenesis bacteria has grown. Mycobacterium tuberculosis, heparin binding hemagglutinin adhesin modulates immune response, making this a subunit vaccine candidate. Through its C-terminal lysine-rich domain, surface antigen interacts with heparan sulfate proteoglycans present in non-phagocytic cells, leading to extrapulmonary dissemination pathogen. study, was expressed as recombinant methylated Rhodococcus...
RESUMENLa raza Holstein es la que más contribuye a producción láctea en Ecuador.Sin embargo, no existen datos de parámetros genéticos describan las características los hatos lecheros del
La Escherichia coli patógena extraintestinal, denominada E. aviar, posee atributos de virulencia específicos que causan infecciones invasivas en aves corral, responsables la Colibacilosis aviar. Los veterinarios tienen opciones restringidas agentes antimicrobianos para su tratamiento, debido a problemas resistencia bacteriana coli, incide indirectamente salud humana. Como alternativa se plantea el uso bacteriófagos con poder bacteriolítico específico contra bacterias enteropatógenas. El...