A5057947982- Bacterial Genetics and Biotechnology
- Bacterial biofilms and quorum sensing
- Genomics and Phylogenetic Studies
- Microbial Natural Products and Biosynthesis
- Legume Nitrogen Fixing Symbiosis
- Enzyme Catalysis and Immobilization
- Biochemical and Molecular Research
- Antibiotic Resistance in Bacteria
- RNA and protein synthesis mechanisms
- Microbial Metabolic Engineering and Bioproduction
- biodegradable polymer synthesis and properties
- Microbial bioremediation and biosurfactants
- Microbial Community Ecology and Physiology
- Bacteriophages and microbial interactions
- Enzyme Structure and Function
- Protein purification and stability
- Viral Infectious Diseases and Gene Expression in Insects
- Fungal and yeast genetics research
- Biofuel production and bioconversion
- Amino Acid Enzymes and Metabolism
- Plant-Microbe Interactions and Immunity
- Glycosylation and Glycoproteins Research
- Monoclonal and Polyclonal Antibodies Research
- Tuberculosis Research and Epidemiology
- Plant nutrient uptake and metabolism
Universidad Nacional Autónoma de México
2015-2024
Universidad Autónoma de la Ciudad de México
2021
University of Warwick
2017
Universidad Autónoma de Zacatecas "Francisco García Salinas"
2015
John Innes Centre
1994-2005
Abstract Background Pseudomonas aeruginosa is an opportunistic pathogen with a high incidence of hospital infections that represents threat to immune compromised patients. Genomic studies have shown that, in contrast other pathogenic bacteria, clinical and environmental isolates do not show particular genomic differences. In addition, genetic variability all the P. strains whose genomes been sequenced extremely low. This low might be explained if constitute subpopulation this bacterial...
In a number of bacterial pathogens, the production virulence factors is induced at 37 °C; this effect often regulated by mRNA structures formed in 5' untranslated region (UTR) that block translation initiation genes environmental temperatures. At °C, RNA become unstable and ribosomes gain access to their binding sites mRNAs. Pseudomonas aeruginosa an important opportunistic pathogen expression many its virulence-associated traits quorum-sensing (QS) response, but temperature on...
Pseudomonas aeruginosa is a major nosocomial pathogen that presents high-level resistance to antibiotics. Its ability cause infections relies on the production of multiple virulence factors. Quorum sensing (QS) regulates expression many these factors through three QS systems: Las, Rhl, and PQS. The Las system positively other two systems, so it at top hierarchized regulation. Nevertheless, clinical environmental strains lack functional have been isolated, and, surprisingly, some them still...
The production of many virulence factors by Pseudomonas aeruginosa is regulated the quorum-sensing (QS) response. In this regulatory network LasR and RhlR, bound to their corresponding autoinducers, play a central role. QS response has hierarchical structure: LasR/3O-C12-HSL activates transcription rhlR, RhlR/C4-HSL several genes, including rhlAB operon, which encodes enzymes responsible for rhamnolipid synthesis. operon located immediately upstream rhlR gene. four start sites, two are in...
Pseudomonas aeruginosa is an environmental bacterium but also opportunistic pathogen. The aim of this work to evaluate the contribution P. LasR and RhlR transcriptional regulators quorum-sensing response (QSR) production virulence factors, its in a mouse abscess model. QSR complex regulatory network that modulates expression several including elastase, pyocyanin rhamnolipids. LasR, when complexed with auto-inducer 3-oxo-dodecanoyl lactone (3O-C12-HSL), produced by LasI, at top cascade since...
ABSTRACT The Azotobacter vinelandii phbBAC genes encode the enzymes for poly-β-hydroxybutyrate (PHB) synthesis. phbR gene, which is located upstream of and in opposite direction , encodes PhbR, a transcriptional activator member AraC family activators. Here we report that mutation reduced PHB accumulation transcription phbB - lacZ fusion. We also transcribed from two overlapping promoters, p B 1 2. region corresponding to −35 overlaps 2 −10 region. In mutant, expression promoter...
pJV1 is an 11 kb, high-copy-number conjugative Streptomyces phaeochromogenes plasmid that replicates by the rolling circle mechanism (RCR). Sequencing combined with functional analysis of deletion, insertion and frameshift mutations was used to characterize genes involved in transfer chromosome mobilization (Cma), single-strand origin for RCR associated strong incompatibility (Sti) determinant. contains two essential whose expression regulated adjacent repressor gene similarity GntR family...
ABSTRACT The gene encoding the 45/47 kDa glycoprotein (Rv1860) of Mycobacterium tuberculosis was expressed in Streptomyces lividans under its own promoter and thiostrepton-inducible P tipA . recombinant protein released into culture medium and, like native protein, migrated as a double band at 45 47 sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) gels. However, contrast to only 47-kDa could be labeled with concanavalin A (ConA). Carbohydrate digestion jack bean α- d...
Pseudomonas aeruginosa is an opportunistic pathogen that presents a complex regulatory network called 'quorum-sensing', which responsible for the transcription of genes coding several traits implicated in its pathogenicity. Strain 148 dolphin isolate has been shown to produce quorum-sensing-regulated virulence and be virulent mouse model, despite fact it contains 20-kbp deletion eliminates from chromosome lasR gene lasI promoter. LasR key quorum-sensing transcriptional regulator that, when...
The two-step model for plant root microbiomes considers soil as the primary microbial source. Active selection of plant's bacterial inhabitants results in a biodiversity decrease toward roots. We collected sixteen samples situ ruderal roots and their soils used these main input single genotype tomatoes grown greenhouse. Our goal was to test influence structuring rhizosphere microbiomes, minimizing environmental variability, while testing multiple species. massively sequenced 16S rRNA shotgun...
A Rhizobium etli Tn5 insertion mutant, LM01, was selected for its inability to use glutamine as the sole carbon and nitrogen source. The in LM01 localized rsh gene, which encodes a member of RelA/SpoT family proteins. mutant affected ability amino acids nitrate sources unable accumulate (p)ppGpp when grown under starvation, opposed wild-type strain, accumulated these conditions. R. gene found restore accumulation DeltarelA DeltaspoT Escherichia coli. Rsh protein consists 744 acids, results...
The bldC locus, required for formation of aerial hyphae in Streptomyces coelicolor, was localized by map-based cloning to the overlap between cosmids D17 and D25 a minimal ordered library. Subcloning sequencing showed that encodes member previously unrecognized family small (58- 78-residue) DNA-binding proteins, related domains MerR transcriptional activators. BldC members are found wide range gram-positive gram-negative bacteria. Constructed DeltabldC mutants were defective differentiation...
Abstract Pseudomonas aeruginosa is a ubiquitous environmental bacterium and an opportunistic pathogen that represents important health hazard. The quorum‐sensing response regulates the expression of several virulence factors involves three regulons: Las, Rhl, Pqs. P. ATCC 9027 strain, which belongs to genetically diverse PA7 clade, contains frame‐shift mutation in pqsR gene encodes transcriptional activator necessary for pyocyanin (PYO) synthesis type strains PAO1 PA14. Here we characterize...