A5101493202- Microbial Fuel Cells and Bioremediation
- Photosynthetic Processes and Mechanisms
- Amino Acid Enzymes and Metabolism
- Microbial Community Ecology and Physiology
- Metalloenzymes and iron-sulfur proteins
- Bacterial Genetics and Biotechnology
- Enzyme Structure and Function
- Lipid Membrane Structure and Behavior
- Microbial Metabolic Engineering and Bioproduction
- Genomics and Phylogenetic Studies
- Bacterial biofilms and quorum sensing
- Lipid metabolism and biosynthesis
- Microbial bioremediation and biosurfactants
- Metal-Catalyzed Oxygenation Mechanisms
- Antioxidant Activity and Oxidative Stress
- Microbial metabolism and enzyme function
- Allergic Rhinitis and Sensitization
- Sphingolipid Metabolism and Signaling
- Peroxisome Proliferator-Activated Receptors
- Wastewater Treatment and Nitrogen Removal
- Anaerobic Digestion and Biogas Production
- Dermatology and Skin Diseases
- Cellular transport and secretion
- Endoplasmic Reticulum Stress and Disease
- Asthma and respiratory diseases
St. Marianna University School of Medicine
2025
Shiga University of Medical Science
2019-2024
University of Tokyo Health Sciences
1991-2024
The University of Tokyo
2014-2023
National Institute for Environmental Studies
2022
Japan Agency for Medical Research and Development
2016
Tohoku University
2001-2015
Milbank Memorial Fund
2010-2013
Japan Science and Technology Agency
2009-2013
University of North Texas
2013
The amino acid sequence deduced from cDNA of the human placental fibronectin receptor is reported. composed two subunits: an alpha subunit 1,008 acids which processed into polypeptides disulfide bonded to one another, and a beta 778 acids. Each has near its COOH terminus hydrophobic segment. This other features suggest structure for in segments serve as transmembrane domains anchoring each membrane dividing large ectodomain short cytoplasmic domain. five elements homologous consensus...
Pseudomonas aeruginosa is a ubiquitously distributed opportunistic pathogen that inhabits soil and water as well animal-, human-, plant-host-associated environments. The ubiquity would be attributed to its very versatile energy metabolism. P. has highly branched respiratory chain terminated by multiple terminal oxidases denitrification enzymes. Five for aerobic respiration have been identified in the cells. Three of them, cbb(3)-1 oxidase, cbb(3)-2 aa(3) are cytochrome c other two, bo(3)...
Cells adhere to vitronectin substrates through a cell surface receptor that recognizes an Arg-Gly-Asp sequence in vitronectin. The is glycoprotein composed of 150-kDa alpha and 115-kDa beta subunit. subunit consists two disulfide-bonded chains 125 kDa 25 kDa. cDNA clones were isolated for the from phage lambda gt11 expression library made with RNA human fibroblast line, IMR-90. identity had been selected based on immunological criteria was verified by comparison DNA protein sequences....
The amino acid sequence of the vitronectin receptor a subunit deduced from cDNA is presented.The defines 1047-amino-acid polypeptide precursor with putative signal sequence, large extracellular domain several sites homologous to calcium binding in other proteins, transmembrane domain, and 32-amino-acid cytoplasmic domain.The 7-kilobase mRNA was found be expressed all cell lines examined, including endothelial cells, K562 HEL leukemia osteosarcoma cells.In two lines, expression mRNA, as well...
alpha-Tocopherol transfer protein was purified from the 10,000 x g supernatant of rat liver. Two isoforms exist, which isoelectric points are 5.0 and 5.1 as determined by chromatofocusing. These two have same molecular weight; both showed weight approx. 30,500 on SDS-polyacrylamide gel electrophoresis. They cannot be distinguished each other amino acid composition or substrate specificity.
Patients with isolated vitamin E deficiency have an impaired ability to incorporate α-tocopherol into lipoproteins in the liver and usually symptoms signs of spinocerebellar dysfunction before adolescence. Accumulated evidence suggests that α-tocopherol–transfer protein, which is presumed function intracellular transport α-tocopherol, abnormal these patients.
Disrupted-In-Schizophrenia-1 (DISC1) is a unique susceptibility gene for major mental conditions, because of the segregation its genetic variant with hereditary psychosis in Scottish pedigree. Genetic association studies reproducibly suggest involvement DISC1 both schizophrenia and bipolar disorder several ethnic groups. The protein multifunctional, pool dynein motor complex required neurite outgrowth PC12 cells as well proper neuronal migration dendritic arborization developing cerebral...
N-3 polyunsaturated fatty acids (PUFAs) have potential cardiovascular benefit, although the mechanisms underlying this effect remain poorly understood. Fat-1 transgenic mice expressing Caenorhabditis elegans n-3 acid desaturase, which is capable of producing PUFAs from n-6 PUFAs, exhibited resistance to pressure overload–induced inflammation and fibrosis, as well reduced cardiac function. Lipidomic analysis revealed selective enrichment eicosapentaenoic (EPA) in fat-1 bone marrow (BM) cells...
In biological systems, the pH in intracellular organelles or tissues is strictly regulated, and differences of are deeply related to key events such as protein degradation, trafficking, renal failure, cancer. Ratiometric fluorescence imaging useful for determination precise values, but existing probes have substantial limitations, inappropriate pKa physiological range, inadequate photobleaching resistance, insufficiently long excitation emission wavelengths. Here we report a versatile...
P4-ATPases translocate aminophospholipids, such as phosphatidylserine (PS), to the cytosolic leaflet of membranes. PS is highly enriched in recycling endosomes (REs) and essential for endosomal membrane traffic. Here, we show that flipping by an RE-localized P4-ATPase required recruitment fission protein EHD1. Depletion ATP8A1 impaired asymmetric transbilayer distribution REs, dissociated EHD1 from generated aberrant tubules appear resistant fission. did not localization cells defective...
α-Tocopherol (vitamin E) transfer protein (α-TTP) regulates the secretion of α-tocopherol from liver cells. Missense mutations some arginine residues at surface α-TTP cause severe vitamin E deficiency in humans, but role these is unclear. Here, we found that wild-type bound phosphatidylinositol phosphates (PIPs), whereas mutants did not. In addition, PIPs target membrane promoted intermembrane by α-TTP. The crystal structure α-TTP-PIPs complex revealed disease-related interacted with...
The ubiquitous opportunistic pathogen Pseudomonas aeruginosa has five aerobic terminal oxidases: bo(3)-type quinol oxidase (Cyo), cyanide-insensitive (CIO), aa3-type cytochrome c (aa3), and two cbb(3)-type oxidases (cbb(3)-1and cbb(3)-2). These are differentially regulated under various growth conditions thought to contribute the survival of this microorganism in a wide variety environmental niches. Here, we constructed multiple mutant strains P. that express only one investigate enzymatic...
Significance Macropinocytosis is a form of endocytosis that accompanied by ruffling plasma membrane and participates in diverse range pathophysiological processes, such as antigen uptake immune cells tumor growth. However, the molecular mechanism underlying this process poorly understood. By exploiting studies fluid-phase Caenorhabditis elegans , we found dephosphorylation phosphoinositide PI(3)P essential for macropinocytosis mammalian cells. We also sequential PI(3,4,5)P 3 → PI(3,4)P 2 PI...
TMEM16K, a membrane protein carrying 10 transmembrane regions, has phospholipid scramblase activity. TMEM16K is localized to intracellular membranes, but whether it actually scrambles phospholipids inside cells not been demonstrated, due technical difficulties in studying lipid distributions. Here, we developed freeze-fracture electron microscopy method that enabled us determine the phosphatidylserine (PtdSer) distribution individual leaflets of cellular membranes. Using this method, found...
We have recently reported that members of the heparin-binding group II subfamily secretory PLA<sub>2</sub>s (sPLA<sub>2</sub>s) (types IIA and V), when transfected into 293 cells, released [<sup>3</sup>H]arachidonic acid (AA) preferentially in response to interleukin-1 (IL-1) acted as "signaling" were functionally coupled with prostaglandin biosynthesis. Here we show these sPLA<sub>2</sub>s type X sPLA<sub>2</sub> behave a different manner, former being more efficiently...
Pseudomonas aeruginosa has five terminal oxidases for aerobic respiration. Two of them, the bo(3) oxidase (Cyo) and cyanide-insensitive (CIO), are quinol other three, cbb(3)-1 (Cbb3-1), cbb(3)-2 (Cbb3-2) aa(3) (Aa3), cytochrome c oxidases. The expression pattern genes these under various growth conditions was investigated by using lacZ transcriptional fusions some novel regulatory issues were found. Aa3 induced starvation conditions. Cyo exposure to nitric oxide-generating reagent...
The partially purified proton-translocating adenosinetriphosphatase [(H+)-ATPase] from clathrin-coated vesicles has been reported to contain eight polypeptides of molecular weights 15,000-116,000 [Xie, X.S., & Stone, D.K. (1986) J. Biol. Chem. 261, 2492-2495]. To determine whether these form a single macromolecular complex, we have isolated three monoclonal antibodies which recognize the reconstitutively active (H+)-ATPase in native, detergent-solubilized state. All precipitate same set...
DISC1 influences susceptibility to psychiatric disease and related phenotypes. Intact functions of its binding partners, NDEL1 NDE1, are critical neurodevelopmental processes aberrant in schizophrenia (SZ). Despite evidence an NDEL1-DISC1 protein interaction, there have been no investigations the gene or relationship between SZ. We genotyped six single-nucleotide polymorphisms (SNPs) 275 Caucasian SZ patients 200 controls tested for association interaction functional SNP Ser704Cys NDEL1....
Cancer associated fibroblasts (CAFs) are responsible for tumor growth, angiogenesis, invasion, and metastasis. Matrix metalloproteinase (MMP)-9 secreted from cancer stroma populated by CAFs is a prerequisite angiogenesis Omega-3 polyunsaturated fatty acids (omega-3 PUFA) have been reported to anti-tumor effects on diverse types of malignancies. Fat-1 mice, which can convert omega-6 omega-3 PUFA independent diet, useful investigate the functions endogenous PUFA. To examine effect...
Host-microbiota interactions create a unique metabolic milieu that modulates intestinal environments. Integration of 16S ribosomal RNA (rRNA) sequences and mass spectrometry (MS)-based lipidomics has great potential to reveal the relationship between bacterial composition complex network in gut. In this study, we conducted untargeted followed by feature-based molecular MS/MS spectral networking characterize gut bacteria-dependent lipid subclasses mice. An estimated 24.8% molecules feces were...
Membrane contact sites (MCSs) serve as a zone for nonvesicular lipid transport by oxysterol-binding protein (OSBP)-related proteins (ORPs). ORPs mediate countertransport, in which two distinct lipids are transported counterdirectionally. How such countertransport controls specific biological functions, however, remains elusive. We report that ORP10 at ER–endosome MCSs regulates retrograde membrane trafficking. ORP10, together with ORP9 and VAP, formed phosphatidylinositol 4-phosphate...