A5031155428- Cervical Cancer and HPV Research
- Endometrial and Cervical Cancer Treatments
- Ovarian cancer diagnosis and treatment
- Endometriosis Research and Treatment
- Reproductive System and Pregnancy
- Cancer, Lipids, and Metabolism
- Peroxisome Proliferator-Activated Receptors
- Uterine Myomas and Treatments
- Immunotherapy and Immune Responses
- Hepatitis B Virus Studies
- Pregnancy and preeclampsia studies
- Cancer, Hypoxia, and Metabolism
- Metabolomics and Mass Spectrometry Studies
- Cancer-related molecular mechanisms research
- Preterm Birth and Chorioamnionitis
- Cancer-related Molecular Pathways
- Genital Health and Disease
- PI3K/AKT/mTOR signaling in cancer
- Cancer Cells and Metastasis
- Reproductive Biology and Fertility
- Cancer Mechanisms and Therapy
- Immune Cell Function and Interaction
- Reproductive tract infections research
- Cancer Genomics and Diagnostics
- Pregnancy and Medication Impact
Nihon University
2016-2025
Japan Pediatric Society
2024
Kyushu University
2020-2023
University of Occupational and Environmental Health Japan
2023
Osaka University
2023
Tohoku University
2023
Keio University
2023
Yamagata University Hospital
2023
Surugadai Nihon University Hospital
2022
The University of Tokyo
2012-2021
The phosphatidylinositol 3'-kinase (PI3K)-AKT pathway is activated in many human cancers and plays a key role cell proliferation survival. A mutation (E17K) the pleckstrin homology domain of AKT1 results constitutive activation by means localisation to plasma membrane. has been reported some tumour types (breast, colorectal, ovarian lung cancers), it interest which other than those possess E17K mutation.We analysed presence 89 endometrial cancer tissue specimens 12 lines PCR direct...
Cervical cancer and its precursor lesion, cervical intraepithelial neoplasia (CIN), are caused by high-risk human papillomavirus (HPV) viral infection highly susceptible to host immunity targeting of HPV proteins, which include both foreign antigens expressed tumors. Immunotherapy that induces Th1 immunoreactivity against proteins is expected take advantage this immunological regression mechanism. However, although immunotherapies for CIN have been developed over the past several decades,...
ABSTRACT Studies of virus neutralization by antibody are a prerequisite for development prophylactic vaccine strategy against human papillomaviruses (HPVs). Using HPV16 and -6 pseudovirions capable inducing β-galactosidase in infected monkey COS-1 cells, we examined the neutralizing activity mouse monoclonal antibodies (MAbs) that recognize surface epitopes minor capsid protein L2. Two MAbs binding to synthetic peptide with L2 sequence amino acids (aa) 108 120 were found inhibit...
ABSTRACT CD1d and CD1d-restricted natural killer T (NKT) cells serve as a bridge between innate adaptive immune responses to microbes. downregulation is utilized by variety of microbes evade detection. We demonstrate here that downregulated in human papillomavirus (HPV)-positive vivo vitro . immunoreactivity was strong HPV-negative normal cervical epithelium but absent HPV16-positive CIN1 HPV6-positive condyloma lesions. used two cell lines for assay; one stably CD1d-transfected established...
The PI3K (phosphatidylinositol-3-kinase)/mTOR (mammalian target of rapamycin) pathway is frequently activated in endometrial cancer through various PI3K/AKT-activating genetic alterations. We examined the antitumor effect NVP-BEZ235—a dual PI3K/mTOR inhibitor—and RAD001—an mTOR inhibitor—in 13 cell lines, all which possess one or more alterations PTEN, PIK3CA, and K-Ras. also combined these compounds with a MAPK inhibitor (PD98059 UO126) lines K-Ras (mutations amplification). PTEN mutant...
Cancer associated fibroblasts (CAFs) are responsible for tumor growth, angiogenesis, invasion, and metastasis. Matrix metalloproteinase (MMP)-9 secreted from cancer stroma populated by CAFs is a prerequisite angiogenesis Omega-3 polyunsaturated fatty acids (omega-3 PUFA) have been reported to anti-tumor effects on diverse types of malignancies. Fat-1 mice, which can convert omega-6 omega-3 PUFA independent diet, useful investigate the functions endogenous PUFA. To examine effect...
// Masakazu Sato 1 , Kei Kawana Katsuyuki Adachi Asaha Fujimoto Mitsuyo Yoshida Hiroe Nakamura Haruka Nishida Tomoko Inoue Ayumi Taguchi Juri Takahashi Satoko Eguchi Aki Yamashita Kensuke Tomio Osamu Wada-Hiraike Katsutoshi Oda Takeshi Nagamatsu Yutaka Osuga Tomoyuki Fujii Department of Obstetrics and Gynecology, Graduate School Medicine, The University Tokyo, Bunkyo-ku, Japan Correspondence to: Kawana, email: kkawana-tky@umin.org Keywords: cancer stem cell (CSC), metabolomics, ovarian...
The histone methyltransferase EZH2, a key epigenetic modifier, is known to be associated with human tumorigenesis. However, the physiological importance of EZH2 and its clinical relevance in endometrial cancer remain unclear. Hence, present study, we investigated expression function cancer. In quantitative real-time PCR analysis 11 cell lines 52 specimens, was significantly overexpressed cells tissues compared that corresponding normal control tissues. Kaplan-Meier survival using data TCGA...
Abstract Aim Endometriosis is a chronic inflammatory disease. Sirtuin 1 ( SIRT1 ) plays role in regulation of inflammation. The endometriosis remains unknown. We here addressed the anti‐inflammatory effects on endometriosis. Methods expression human ovarian endometriomas and eutopic endometria were examined using immunohistochemistry reverse transcription polymerase chain reaction RT–PCR ). Endometriotic stromal cells ESC obtained from exposed to either resveratrol or sirtinol, an activator...
Human papillomavirus vaccines are being introduced worldwide and expected to reduce the incidence of cervical cancer. Here we report a cross-sectional study using validated human genotyping method reveal prevalence genotype distribution in Japanese women with intraepithelial neoplasia Grade 2/3 invasive Cervical exfoliated cells were collected from 647 patients abnormal histology (cervical 2, n = 164; 3, 334; cancer, 149), subjected PGMY-PCR-based assay. The association between infection...
PTEN inactivation is the most frequent genetic aberration in endometrial cancer. One of phosphatase-independent roles associated with homologous recombination (HR) nucleus. Poly (ADP-ribose) polymerase (PARP) plays key repair DNA single-strand breaks, and a PARP inhibitor induces synthetic lethality cancer cells HR deficiency. We examined anti-tumor activity olaparib, inhibitor, its correlation between sensitivity status cell lines. The response to olaparib was evaluated using clonogenic...
Cancer-associated fibroblasts (CAFs) play an important role in cancer expansion and progression tumor microenvironment (TME), via both direct indirect interactions. Natural killer (NK) cells a crucial anticancer immunity. We investigated the inhibitory effects of CAFs on NK cell activity. were isolated from endometrial tissue, while normal (NEFs) obtained endometrium with no pathological abnormality. allogenic healthy volunteers. or NEFs co-cultured at NK/fibroblast ratio 1:1 without...
Problem Immune tolerance to endometriotic cells is important promote endometriosis. Tryptophan 2,3-dioxygenase (TDO) enhances immune by catabolizing tryptophan kynurenine. We studied whether interleukin-1β (IL-1β), a typical endometriosis-associated cytokine, affects the expression of TDO and catabolism in endometrioma stromal (ESCs). also involved IL-1β-induced secretion IL-6 IL-8 ESCs. Method study Nineteen patients reproductive age with normal menstrual cycles were recruited. Primary...
Ovarian clear cell carcinoma (CCC) is generally associated with chemoresistance and poor clinical outcome, even early diagnosis; whereas high-grade serous carcinomas (SCs) endometrioid (ECs) are commonly chemosensitive at advanced stages. Although an integrated genomic analysis of SC has been performed, conclusive views on copy number expression profiles for CCC still limited. In this study, we performed single nucleotide polymorphism 57 epithelial ovarian cancers (31 CCCs, 14 SCs, 12 ECs)...