A5091207706- Cervical Cancer and HPV Research
- Ovarian cancer diagnosis and treatment
- Endometrial and Cervical Cancer Treatments
- Cancer-related Molecular Pathways
- Acute Lymphoblastic Leukemia research
- Uterine Myomas and Treatments
- PI3K/AKT/mTOR signaling in cancer
- Hippo pathway signaling and YAP/TAZ
- Ubiquitin and proteasome pathways
- Childhood Cancer Survivors' Quality of Life
- Hematopoietic Stem Cell Transplantation
- Cancer Genomics and Diagnostics
- Wnt/β-catenin signaling in development and cancer
- Cancer Mechanisms and Therapy
- Epigenetics and DNA Methylation
- Chromatin Remodeling and Cancer
- Endometriosis Research and Treatment
- Acute Myeloid Leukemia Research
- Sarcoma Diagnosis and Treatment
- Viral-associated cancers and disorders
- Cancer-related gene regulation
- Intracranial Aneurysms: Treatment and Complications
- Lymphoma Diagnosis and Treatment
- Sirtuins and Resveratrol in Medicine
- Estrogen and related hormone effects
Kagoshima University
2015-2024
Sanno Medical Center
2024
Kagoshima University Hospital
2021-2024
Kyushu Institute of Technology
2024
Kindai University
2023
Sapporo City General Hospital
2023
University of Shizuoka
2023
Yazaki (United States)
2022
Kyushu University
2022
Hiroshima University
2022
AbstractThe high-risk human papillomavirus (HPV) E6 proteins stimulate the ubiquitination and degradation of p53, dependent on E6AP ubiquitin-protein ligase. Other have also been shown to be targeted for by E6, including hDlg, homolog Drosophila melanogaster Discs large (Dlg) tumor suppressor. We show here that Scribble (Vartul) (hScrib) suppressor protein is E6-E6AP complex in vitro expression induces hScrib vivo. Characterization E6AP-E6-hScrib indicated binds directly binding mediated PDZ...
Purpose The objective of this study was to assess clinical outcomes and fertility in patients treated conservatively for unilateral stage I invasive epithelial ovarian cancer (EOC). Patients Methods A multi-institutional retrospective investigation undertaken identify with EOC fertility-sparing surgery. Favorable histology defined as grade 1 or 2 adenocarcinoma, excluding clear cell histology. Results total 211 (stage IA, n = 126; IC, 85) were identified from 30 institutions. Median duration...
The phosphatidylinositol 3'-kinase (PI3K)-AKT pathway is activated in many human cancers and plays a key role cell proliferation survival. A mutation (E17K) the pleckstrin homology domain of AKT1 results constitutive activation by means localisation to plasma membrane. has been reported some tumour types (breast, colorectal, ovarian lung cancers), it interest which other than those possess E17K mutation.We analysed presence 89 endometrial cancer tissue specimens 12 lines PCR direct...
Abstract Background Resveratrol is a natural polyphenolic compound known for its beneficial effects on energy homeostasis, and it also has multiple properties, including anti-oxidant, anti-inflammatory, anti-tumor activities. Recently, silent information regulator genes (Sirtuins) have been identified as targets of resveratrol. Sirtuin 1 (SIRT1), originally found an NAD + -dependent histone deacetylase, principal modulator pathways downstream calorie restriction, the activation SIRT1...
Patients with epithelial ovarian carcinoma upstaged from Stage I/II to IIIC based on lymph node involvement are known have poor prognoses. The authors investigated whether systematic aortic and pelvic lymphadenectomy would affect the prognoses of these patients.During period 1987-1996, 103 patients in I-III underwent optimal cytoreductive surgery at initial surgery. All except for those IA received adjuvant cisplatin-based chemotherapy after Of 67 intraperitoneal tumors limited pelvis, 14...
Recent genome-wide analysis has demonstrated that somatic mutations in ARID1A (BAF250) are the most common molecular genetic changes ovarian clear cell carcinoma (OCCC). mutations, which occur approximately half of OCCC cases, lead to deletion encoded protein and inactivation putative tumor suppressor. In this study, we determined significance loss immunoreactivity with respect several clinicopathological features a total 149 OCCCs. First, immunohistochemistry showed concordance mutational...
The PI3K (phosphatidylinositol-3-kinase)/mTOR (mammalian target of rapamycin) pathway is frequently activated in endometrial cancer through various PI3K/AKT-activating genetic alterations. We examined the antitumor effect NVP-BEZ235—a dual PI3K/mTOR inhibitor—and RAD001—an mTOR inhibitor—in 13 cell lines, all which possess one or more alterations PTEN, PIK3CA, and K-Ras. also combined these compounds with a MAPK inhibitor (PD98059 UO126) lines K-Ras (mutations amplification). PTEN mutant...
Recently, a LAP protein, scribble, was identified in Drosophila epithelia as basolateral protein that controls the apical-basolateral polarity. Loss of scribble causes disorganisation and overgrowth epithelia. Scribble has human homologue, (hScrib), which is substrate ubiquitin-mediated degradation by papillomavirus E6 E6AP ubiquitin-protein ligase. In present study, we revealed hScrib localised to regions epithelial cell line MDCK uterine cervical tissues immunofluorescence. Human...
DBC1/KIAA1967 (deleted in breast cancer 1) is a putative tumour-suppressor gene cloned from heterozygously deleted region specimens. Caspase-dependent processing of DBC1 promotes apoptosis, and depletion endogenous negatively regulates p53-dependent apoptosis through its specific inhibition SIRT1. Hereditary ovarian susceptibility product BRCA1, by binding to the promoter SIRT1, positive regulator SIRT1 expression. A physical interaction between BRCA1 was investigated both vivo vitro. To...
Microcystic stromal tumor (MCST) is a recently described subtype of ovarian characterized by prominent microcystic histologic pattern and diffuse immunoreactivity for CD10 vimentin. However, its pathobiology, particularly histogenesis, remains largely unclear. Here, we report 2 cases MCST, in which have performed extensive histologic, immunohistochemical, genetic investigations to determine distinct nature among neoplasms. The patients were 32 41 years age. Both tumors solid cystic masses...
PTEN inactivation is the most frequent genetic aberration in endometrial cancer. One of phosphatase-independent roles associated with homologous recombination (HR) nucleus. Poly (ADP-ribose) polymerase (PARP) plays key repair DNA single-strand breaks, and a PARP inhibitor induces synthetic lethality cancer cells HR deficiency. We examined anti-tumor activity olaparib, inhibitor, its correlation between sensitivity status cell lines. The response to olaparib was evaluated using clonogenic...
Highlights•Scrib is asymmetrically distributed in dividing satellite cells•Satellite cell fate dictated by the level of Scrib•Scrib mediates growth factor signaling activated cells•Scrib indispensable for muscle regeneration vivoSummarySatellite cells are resident skeletal stem that supply myonuclei homeostasis, hypertrophy, and repair adult muscle. Scrib one major cell-polarity proteins, acting as a potent tumor suppressor epithelial cells. Here, we show also controls satellite-cell-fate...
Ovarian clear cell carcinoma (CCC) is generally associated with chemoresistance and poor clinical outcome, even early diagnosis; whereas high-grade serous carcinomas (SCs) endometrioid (ECs) are commonly chemosensitive at advanced stages. Although an integrated genomic analysis of SC has been performed, conclusive views on copy number expression profiles for CCC still limited. In this study, we performed single nucleotide polymorphism 57 epithelial ovarian cancers (31 CCCs, 14 SCs, 12 ECs)...
The SFRP1 gene on chromosome 8p11.2 encodes a Wnt signaling antagonist, and was recently demonstrated to be new tumor suppressor that is inactivated by promoter methylation in human colon cancers. Here, we analyzed of the ovarian cancers, which loss heterozygosity 8p frequently observed involvement pathway has been suggested. Methylation‐specific PCR (MSP) analysis showed four 13 cancer cell lines two 17 primary cancers had methylated , while an immortalized epithelial line, HOSE, seven...
Recently, we have identified human scribble (hScrib), homolog of the Drosophila tumor suppressor Scribble, as a substrate papillomavirus E6 oncoproteins for ubiquitin‐mediated degradation dependent on ubiquitin‐protein ligase E6AP. Human classified LAP protein containing l eucine‐rich repeats nd P DZ domains, interacts with through its PDZ domains and C‐terminal domain‐binding motif protein. Interaction between Discs Large (hDlg), which is degradation, adenomatous polyposis coli (APC) has...
DS-7423, a novel, small-molecule dual inhibitor of phosphatidylinositol-3-kinase (PI3K) and mammalian target rapamycin (mTOR), is currently in phase I clinical trials for solid tumors. Although DS-7423 potently inhibits PI3Kα (IC50 = 15.6 nM) mTOR 34.9 nM), it also other isoforms class PI3K values: PI3Kβ 1,143 nM; PI3Kγ 249 PI3Kδ 262 nM). The PI3K/mTOR pathway frequently activated ovarian clear cell adenocarcinomas (OCCA) through various mutations that activate PI3K-AKT signaling. Here, we...
Despite the fact that approximately ten types of human papillomavirus (HPV) are associated with cervical carcinoma, question whether HPV type present in carcinoma is related to clinical behavior had yet be answered when this study was conducted.The presence and were determined by polymerase chain reaction (PCR)-based assay (LI-PCR) 146 carcinomas. Clinical features compared among following 4 groups: Group 18 (27 patients positive for 18), 16 (55 16), O (47 other types), N (17 negative...
The presence of human papillomavirus (HPV) DNA in almost all the cervical carcinomas is one most compelling evidence for viral carcinogenesis. HPVs are thought to induce carcinoma likely through expression E6 and E7 genes presumably by inactivating tumor suppressor proteins, p53 pRb, respectively. Thus far, HPV transcripts have been identified only carcinoma-derived cell lines harboring type 16 or 18, a limited number neoplasia specimens positive 16, 33 51. To see whether an essential...
Drosophila tumor suppressor Scribble has been identified as an apical‐basolateral polarity determinant in epithelia. A human homolog of Scribble, (hScrib), a protein targeted by papillomavirus E6 for the ubiquitin‐mediated degradation dependent on E6AP, cellular ubiquitin‐protein ligase. Human is classified LAP protein, having leucine‐rich repeats (LRRs) and PDZ domains. We investigated whether hScrib, which thought to have role determination based data its homolog, involved cell‐cycle...