A5010124046- Ovarian cancer diagnosis and treatment
- Endometrial and Cervical Cancer Treatments
- Cancer Genomics and Diagnostics
- PI3K/AKT/mTOR signaling in cancer
- Cervical Cancer and HPV Research
- PARP inhibition in cancer therapy
- Uterine Myomas and Treatments
- Cancer-related Molecular Pathways
- Chronic Lymphocytic Leukemia Research
- Endometriosis Research and Treatment
- Cancer Mechanisms and Therapy
- BRCA gene mutations in cancer
- Cancer Cells and Metastasis
- Cancer-related molecular mechanisms research
- Molecular Biology Techniques and Applications
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Sarcoma Diagnosis and Treatment
- Chromatin Remodeling and Cancer
- Reproductive System and Pregnancy
- Cancer-related gene regulation
- Lung Cancer Treatments and Mutations
- Melanoma and MAPK Pathways
- Reproductive Biology and Fertility
- Immunotherapy and Immune Responses
The University of Tokyo
2016-2025
University of Tokyo Hospital
2005-2024
Genomics (United Kingdom)
2023
Institute of Genomics and Integrative Biology
2021
University of California, San Francisco
2005-2008
Cancer Research Institute
2005-2006
Saitama Cancer Center
2004
Tokyo University of Science
1998-2000
Textile Research Institute
1979
Abstract The phosphatidylinositol 3′-kinase (PI3K) pathway is activated in many human cancers. In addition to inactivation of the PTEN tumor suppressor gene, mutations or amplifications catalytic subunit α PI3K (PIK3CA) have been reported. However, coexistence these two genes seems exceedingly rare. As occur at high frequency endometrial carcinoma, we screened 66 primary carcinomas for helical and domains PIK3CA. We identified a total 24 (36%) this gene PIK3CA/PTEN (26%). PIK3CA were more...
Abstract Mutations in genes functioning different pathways frequently occur together the same cancer, whereas mutations pathway tend to be mutually exclusive. However, majority of colon, breast, and endometrial cancers that possess PIK3CA, catalytic subunit p110α phosphatidylinositol 3′-kinase (PI3K), also or alterations upstream PI3K such as Ras, ERBB2/ERBB3, PTEN. PIK3CA almost exclusively invasive tumors, early-stage late-stage suggesting mutation is a event may augment earlier activation...
The phosphatidylinositol 3'-kinase (PI3K)-AKT pathway is activated in many human cancers and plays a key role cell proliferation survival. A mutation (E17K) the pleckstrin homology domain of AKT1 results constitutive activation by means localisation to plasma membrane. has been reported some tumour types (breast, colorectal, ovarian lung cancers), it interest which other than those possess E17K mutation.We analysed presence 89 endometrial cancer tissue specimens 12 lines PCR direct...
Abstract Background Resveratrol is a natural polyphenolic compound known for its beneficial effects on energy homeostasis, and it also has multiple properties, including anti-oxidant, anti-inflammatory, anti-tumor activities. Recently, silent information regulator genes (Sirtuins) have been identified as targets of resveratrol. Sirtuin 1 (SIRT1), originally found an NAD + -dependent histone deacetylase, principal modulator pathways downstream calorie restriction, the activation SIRT1...
Uterine adenomyosis is a benign disorder that often co-occurs with endometriosis and/or leiomyoma, and impairs quality of life. The genomic features are unknown. Here we apply next-generation sequencing to (70 individuals 192 multi-regional samples), as well co-occurring leiomyoma endometriosis, find recurring KRAS mutations in 26/70 (37.1%) cases. Multi-regional reveals oligoclonality adenomyosis, some also detected normal endometrium endometriosis. more frequent cases low progesterone...
Abstract Background To promote precision oncology in clinical practice, the Japanese Society of Medical Oncology, Clinical and Cancer Association, jointly published “Clinical practice guidance for next-generation sequencing cancer diagnosis treatment” 2017. Since new information on genomic medicine has emerged since 1st edition was released, including reimbursement NGS-based multiplex gene panel tests 2019, revision made. Methods A working group organized with 33 researchers from designated...
The PI3K (phosphatidylinositol-3-kinase)/mTOR (mammalian target of rapamycin) pathway is frequently activated in endometrial cancer through various PI3K/AKT-activating genetic alterations. We examined the antitumor effect NVP-BEZ235—a dual PI3K/mTOR inhibitor—and RAD001—an mTOR inhibitor—in 13 cell lines, all which possess one or more alterations PTEN, PIK3CA, and K-Ras. also combined these compounds with a MAPK inhibitor (PD98059 UO126) lines K-Ras (mutations amplification). PTEN mutant...
Cancer associated fibroblasts (CAFs) are responsible for tumor growth, angiogenesis, invasion, and metastasis. Matrix metalloproteinase (MMP)-9 secreted from cancer stroma populated by CAFs is a prerequisite angiogenesis Omega-3 polyunsaturated fatty acids (omega-3 PUFA) have been reported to anti-tumor effects on diverse types of malignancies. Fat-1 mice, which can convert omega-6 omega-3 PUFA independent diet, useful investigate the functions endogenous PUFA. To examine effect...
Tumor molecular profiling is becoming a standard of care for patients with cancer, but the optimal platform cancer sequencing remains undetermined. We established comprehensive assay, Todai OncoPanel ( TOP ), which consists DNA and RNA hybridization capture‐based next‐generation panels. A novel method target enrichment, named junction capture method, was developed panel to accurately cost‐effectively detect 365 fusion genes as well aberrantly spliced transcripts. The can also measure...
Endometrial cancer is a ubiquitous gynecological disease with increasing global incidence. Therefore, despite the lack of an established screening technique to date, early diagnosis endometrial assumes critical importance. This paper presents artificial-intelligence-based system detect regions affected by automatically from hysteroscopic images. In this study, 177 patients (60 normal endometrium, 21 uterine myoma, 60 polyp, 15 atypical hyperplasia, and cancer) history hysteroscopy were...
Comprehensive cancer genome profiling (CGP) has been nationally reimbursed in Japan since June 2019. Less than 10% of the patients have reported to undergo recommended treatment. Todai OncoPanel (TOP) is a dual DNA-RNA panel as well paired tumor-normal matched test. Two hundred underwent TOP part Advanced Medical Care B with approval from Ministry Health, Labour and Welfare between September 2018 December Tests were carried out cancers without standard treatment or when had already undergone...
IMPORTANCE Precision oncology using comprehensive genomic profiling (CGP) by next-generation sequencing is aimed at companion diagnosis and profiling. The clinical utility of CGP before the standard care (SOC) still not resolved, more evidence needed. OBJECTIVE To investigate (FoundationOne CDx [F1CDx]) in patients with previously untreated metastatic or recurrent solid tumors. DESIGN, Setting, Participants This multicenter, prospective, observational cohort study enrolled advanced tumors...
DBC1/KIAA1967 (deleted in breast cancer 1) is a putative tumour-suppressor gene cloned from heterozygously deleted region specimens. Caspase-dependent processing of DBC1 promotes apoptosis, and depletion endogenous negatively regulates p53-dependent apoptosis through its specific inhibition SIRT1. Hereditary ovarian susceptibility product BRCA1, by binding to the promoter SIRT1, positive regulator SIRT1 expression. A physical interaction between BRCA1 was investigated both vivo vitro. To...
Microcystic stromal tumor (MCST) is a recently described subtype of ovarian characterized by prominent microcystic histologic pattern and diffuse immunoreactivity for CD10 vimentin. However, its pathobiology, particularly histogenesis, remains largely unclear. Here, we report 2 cases MCST, in which have performed extensive histologic, immunohistochemical, genetic investigations to determine distinct nature among neoplasms. The patients were 32 41 years age. Both tumors solid cystic masses...
The histone methyltransferase EZH2, a key epigenetic modifier, is known to be associated with human tumorigenesis. However, the physiological importance of EZH2 and its clinical relevance in endometrial cancer remain unclear. Hence, present study, we investigated expression function cancer. In quantitative real-time PCR analysis 11 cell lines 52 specimens, was significantly overexpressed cells tissues compared that corresponding normal control tissues. Kaplan-Meier survival using data TCGA...
PTEN inactivation is the most frequent genetic aberration in endometrial cancer. One of phosphatase-independent roles associated with homologous recombination (HR) nucleus. Poly (ADP-ribose) polymerase (PARP) plays key repair DNA single-strand breaks, and a PARP inhibitor induces synthetic lethality cancer cells HR deficiency. We examined anti-tumor activity olaparib, inhibitor, its correlation between sensitivity status cell lines. The response to olaparib was evaluated using clonogenic...
// Masakazu Sato 1 , Kei Kawana Katsuyuki Adachi Asaha Fujimoto Mitsuyo Yoshida Hiroe Nakamura Haruka Nishida Tomoko Inoue Ayumi Taguchi Juri Takahashi Satoko Eguchi Aki Yamashita Kensuke Tomio Osamu Wada-Hiraike Katsutoshi Oda Takeshi Nagamatsu Yutaka Osuga Tomoyuki Fujii Department of Obstetrics and Gynecology, Graduate School Medicine, The University Tokyo, Bunkyo-ku, Japan Correspondence to: Kawana, email: kkawana-tky@umin.org Keywords: cancer stem cell (CSC), metabolomics, ovarian...
Cancer-associated fibroblasts (CAFs) play an important role in cancer expansion and progression tumor microenvironment (TME), via both direct indirect interactions. Natural killer (NK) cells a crucial anticancer immunity. We investigated the inhibitory effects of CAFs on NK cell activity. were isolated from endometrial tissue, while normal (NEFs) obtained endometrium with no pathological abnormality. allogenic healthy volunteers. or NEFs co-cultured at NK/fibroblast ratio 1:1 without...