A5074211101- Lipid Membrane Structure and Behavior
- Sphingolipid Metabolism and Signaling
- Cellular transport and secretion
- Erythrocyte Function and Pathophysiology
- Lipid metabolism and biosynthesis
- Lysosomal Storage Disorders Research
- Glycosylation and Glycoproteins Research
- Cholesterol and Lipid Metabolism
- Caveolin-1 and cellular processes
- Nuclear Physics and Applications
- Monoclonal and Polyclonal Antibodies Research
- Cancer, Lipids, and Metabolism
- Force Microscopy Techniques and Applications
- Cancer, Hypoxia, and Metabolism
- Advanced Fluorescence Microscopy Techniques
- RNA Interference and Gene Delivery
- Peroxisome Proliferator-Activated Receptors
- Nanopore and Nanochannel Transport Studies
- Nuclear physics research studies
- ATP Synthase and ATPases Research
- Mitochondrial Function and Pathology
- Endoplasmic Reticulum Stress and Disease
- Photoreceptor and optogenetics research
- Advanced biosensing and bioanalysis techniques
- Solid-state spectroscopy and crystallography
RIKEN
2016-2025
Université de Strasbourg
2016-2025
Centre National de la Recherche Scientifique
2016-2025
Tohoku University
1997-2024
Yakult Honsha (Japan)
2024
Pharmac
2024
Pioneer (United States)
2024
Laboratoire de Biophotonique et Pharmacologie
2017-2024
Pioneer (Japan)
2024
Inserm
2008-2023
Exosomes are small vesicles secreted from multivesicular bodies, which able to stimulate the immune system leading tumour cell eradication. We have analysed lipids of exosomes either upon stimulation rat mast cells (RBL-2H3 cells), or constitutively human dendritic cells. As compared with parent cells, displayed an enrichment in sphingomyelin, but not cholesterol. Phosphatidylcholine content was decreased, noted disaturated molecular species as phosphatidylethanolamines....
Abstract Stimulator of interferon genes (STING) is essential for the type I response against DNA pathogens. In to presence and/or cyclic dinucleotides, STING translocates from endoplasmic reticulum perinuclear compartments. However, role this subcellular translocation remains poorly defined. Here we show that palmitoylation at Golgi activation STING. Treatment with inhibitor 2-bromopalmitate (2-BP) suppresses and abolishes response. Mutation two membrane-proximal Cys residues (Cys88/91)...
Very little is known about the biophysical properties and lipid or protein composition of membrane domains presumably present in endocytic biosynthetic organelles. Here we analyzed late endosomes by suborganellar fractionation absence detergent. We found that internal membranes this multivesicular organelle can be separated from limiting each population exhibited a defined composition. Our data also indicated may consist at least two populations, containing primarily phosphatidylcholine...
Abstract St AR ‐related lipid transfer domain‐3 ( STARD 3) is a sterol‐binding protein that creates endoplasmic reticulum ER )–endosome contact sites. How this protein, at the crossroad between sterol uptake and synthesis pathways, impacts intracellular distribution of was ill‐defined. Here, by using in situ cholesterol labeling quantification, we demonstrated 3 induces accumulation endosomes expense plasma membrane. 3‐mediated routing depends both on its activity ability to create –endosome...
An extra sugar protects Many microbial pathogens produce proteins that are toxic to the cells they targeting. Broad-leaved plants susceptible NLP (necrosis and ethylene-inducing peptide 1–like protein) toxins. Lenarčič et al. identified receptors for toxins be GIPC (glycosylinositol phosphorylceramide) sphingolipids (see Perspective by Van den Ackerveken). Their findings reveal why these only attack broad-leaved (so-called eudicots): If sphingolipid carries just two hexoses, as is case...
Cytochrome c (cyt. c) is a proapoptotic factor that binds preferentially to cardiolipin (CL), mitochondrial lipid, but not hydroperoxide (CL-OOH). Cyt. had bound CL liposomes was liberated on peroxidation of the by radical. The generation CL-OOH in mitochondria occurred before release cyt. rat basophile leukaemia (RBL)2H3 cells been induced undergo apoptosis exposure hypoglycaemia with 2-deoxyglucose (2DG). amount prepared from 2DG-treated lower than untreated cells. completely suppressed...
In the present study, we show that in human endothelial cells tetraspanin CD63/lamp3 distributes predominantly to internal membranes of multivesicular–multilamellar late endosomes, which contain unique lipid lysobisphosphatidic acid. Some is also Weibel–Palade bodies, characteristic secretory organelle these cells. We find molecules can be transported from endosomes bodies and thus cycles between endocytic biosynthetic compartments; however, movement much slower than P-selectin, known cycle...
Ro09-0198 is a tetracyclic polypeptide of 19 amino acids that recognizes strictly the structure phosphatidylethanolamine (PE) and forms tight equimolar complex with PE on biological membranes. Using cyclic peptide coupled fluorescence-labeled streptavidin, we have analyzed cell surface localization in dividing Chinese hamster ovary cells. We found was exposed specifically at cleavage furrow during late telophase cytokinesis. only no alteration distribution plasma membrane-bound observed...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTGlycosphingolipid-enriched, detergent-insoluble complexes in protein sorting epithelial cellsKlaus Fiedler, Toshihide Kobayashi, Teymuras V. Kurzchalia, and Kai SimonsCite this: Biochemistry 1993, 32, 25, 6365–6373Publication Date (Print):June 29, 1993Publication History Published online1 May 2002Published inissue 29 June 1993https://doi.org/10.1021/bi00076a009RIGHTS & PERMISSIONSArticle Views360Altmetric-Citations194LEARN ABOUT THESE...
Cytochrome c (cyt. c) is a proapoptotic factor that binds preferentially to cardiolipin (CL), mitochondrial lipid, but not hydroperoxide (CL-OOH). Cyt. had bound CL liposomes was liberated on peroxidation of the by radical. The generation CL-OOH in mitochondria occurred before release cyt. rat basophile leukaemia (RBL)2H3 cells been induced undergo apoptosis exposure hypoglycaemia with 2-deoxyglucose (2DG). amount prepared from 2DG-treated lower than untreated cells. completely suppressed...
Abstract A number of epidemiologic studies have indicated a strong association between dietary fat intake and prostate cancer development, suggesting that lipid metabolism plays some important roles in carcinogenesis its progression. In this study, through our genome-wide gene expression analysis clinical cells, we identified novel lipogenic gene, ELOVL7, coding possible long-chain fatty acid elongase, as overexpressed cells. ELOVL7 is regulated by the androgen pathway SREBP1, well other...
Cytokinesis is a crucial step in the creation of two daughter cells by formation and ingression cleavage furrow. Here, we show that sphingomyelin (SM), one major sphingolipids mammalian cells, required for localization phosphatidylinositol-4,5-bisphosphate (PIP2) to furrow during cytokinesis. Real-time observation with labeled SM-specific protein, lysenin, revealed SM concentrated outer leaflet at time Superresolution fluorescence microscopy analysis indicates transbilayer colocalization...
P4-ATPases translocate aminophospholipids, such as phosphatidylserine (PS), to the cytosolic leaflet of membranes. PS is highly enriched in recycling endosomes (REs) and essential for endosomal membrane traffic. Here, we show that flipping by an RE-localized P4-ATPase required recruitment fission protein EHD1. Depletion ATP8A1 impaired asymmetric transbilayer distribution REs, dissociated EHD1 from generated aberrant tubules appear resistant fission. did not localization cells defective...
There is a limited number of methods to examine transbilayer lipid distribution in biomembranes. We employed freeze-fracture replica-labelling immunoelectron microscopy combination with lipid-binding proteins and peptide both lateral various phospholipids mammalian cells. Our results indicate that are exclusively distributed either the outer or inner leaflet human red blood cell (RBC) membranes. In contrast, nucleated cells, such as skin fibroblasts neutrophils, sphingomyelin was leaflets...
Sphingomyelin (SM) has been proposed to form cholesterol-dependent raft domains and sphingolipid in the plasma membrane (PM). How SM contributes formation function of these remains unknown, primarily because scarcity suitable fluorescent analogs. We developed new analogs by conjugating a hydrophilic fluorophore choline headgroup without eliminating its positive charge, via nonaethylene glycol linker. The behaved similarly native terms their partitioning behaviors artificial liquid...
Accumulating evidence indicates that membrane lipids are not randomly distributed but rather form specific domains. In particular, raft-like microdomains composed of cholesterol and sphingolipids attracting a lot attention. These thought to serve as platforms for signal transduction molecular trafficking, it is difficult elucidate their detailed structure since reported size smaller than the resolution light microscopy. To circumvent this limitation, we designed probes cholesterol-...
Little is known about the organization of lipids in biomembranes. Lipid rafts are defined as sphingolipid- and cholesterol-rich clusters membrane. Details lipid distribution not well characterized mainly because a lack appropriate probes. Ganglioside GM1-specific protein, cholera toxin, has long been only probe rafts. Recently it was shown that earthworm lysenin, specifically recognizes sphingomyelin-rich membrane domains. Binding lysenin to sphingomyelin accompanied by oligomerization toxin...
Engagement of the Fas receptor (CD95) initiates multiple signaling pathways that lead to apoptosis, such as formation death-inducing complex (DISC), activation caspase cascades, and generation lipid messenger, ceramide. Sphingomyelin (SM) is a major component rafts, which are specialized structures enhance efficiency membrane main source However, functions SM in Fas-mediated apoptosis have yet be clearly defined, responsible genes not been identified. After cloning gene for synthesis, SMS1,...