A5090504298- Glycosylation and Glycoproteins Research
- Caveolin-1 and cellular processes
- Platelet Disorders and Treatments
- Lipid Membrane Structure and Behavior
- Blood properties and coagulation
- Erythrocyte Function and Pathophysiology
- Sphingolipid Metabolism and Signaling
- Cellular transport and secretion
- Cell Adhesion Molecules Research
- Proteoglycans and glycosaminoglycans research
- Galectins and Cancer Biology
- Receptor Mechanisms and Signaling
- Nanopore and Nanochannel Transport Studies
- Molecular Biology Techniques and Applications
- Protein Structure and Dynamics
- Protein Kinase Regulation and GTPase Signaling
- Immune Cell Function and Interaction
- Neurobiology and Insect Physiology Research
- Lipid metabolism and biosynthesis
- Cellular Mechanics and Interactions
- Pancreatic function and diabetes
- Blood Coagulation and Thrombosis Mechanisms
- Forensic and Genetic Research
- Hemophilia Treatment and Research
- Monoclonal and Polyclonal Antibodies Research
Tokyo Metropolitan Institute of Medical Science
2014-2025
Hyogo Prefectural Police
2011-2013
Japan Science and Technology Agency
2003-2007
Toshiba (Japan)
1974-2002
Osaka Prefecture University
1996
The University of Tokyo
1991-1993
Tokyo University of Science
1990-1993
Tokyo Institute of Technology
1986-1989
There is a limited number of methods to examine transbilayer lipid distribution in biomembranes. We employed freeze-fracture replica-labelling immunoelectron microscopy combination with lipid-binding proteins and peptide both lateral various phospholipids mammalian cells. Our results indicate that are exclusively distributed either the outer or inner leaflet human red blood cell (RBC) membranes. In contrast, nucleated cells, such as skin fibroblasts neutrophils, sphingomyelin was leaflets...
Choline supplies methyl groups for regeneration of methionine and the donor S-adenosylmethionine in liver. Here, we report that catabolism membrane phosphatidylcholine (PC) into water-soluble glycerophosphocholine (GPC) by phospholipase/lysophospholipase PNPLA8-PNPLA7 axis enables endogenous choline stored hepatic PC to be utilized metabolism. PNPLA7-deficient mice show marked decreases GPC, choline, several metabolites related cycle, accompanied various signs insufficiency, including growth...
Association of gangliosides with specific proteins in the central nervous system was examined by co-immunoprecipitation anti-ganglioside antibody. Protein kinase activity detected precipitates monoclonal antibody to ganglioside GD3 (R24) from membranal fraction rat brain. Using vitro assay, several phosphorylated 40, 53, 56, and 80 kDa were isolated gel electrophoresis. Of these proteins, 53 56 (p53/56) identified as two isoforms Src family tyrosine Lyn, based on co-migration during...
The association of ganglioside GD3 with TAG-1, a glycosylphosphatidylinositol-anchored neuronal cell adhesion molecule, was examined by coimmunoprecipitation experiments. Previously, we have shown that the anti-ganglioside antibody (R24) immunoprecipitated Src family kinase Lyn from rat cerebellum, and R24 treatment primary cerebellar cultures induced activation rapid tyrosine phosphorylation an 80-kDa protein (p80). We now report coimmunoprecipitates 135-kDa (p135) cultures. Treatment...
Using an expression cloning approach, we have isolated a cDNA encoding GD3 synthase (CMP-NeuAc:NeuAc alpha 2-3Gal beta 1-4Glc 1-1'Cer 2,8-sialyltransferase, EC 2.4.99.8), which is key regulatory enzyme determining the prominence of ganglioside biosynthesis pathway. The cloned encodes 341-amino acid protein containing single transmembrane domain at its N-terminal region, suggesting that has type II topology. sequence 2,8-sialyltransferase showed high level similarity with other...
We have identified a novel phospholipase A1, named mPA-PLA1beta, which is specifically expressed in human testis and characterized it biochemically together with previously mPA-PLA1alpha. The sequence of mPAPLA1beta encodes 460-amino acid protein containing lipase domain significant homology to the phosphatidic (PA)-selective PLA1, mPA-PLA1beta contains short lid deleted beta9 loop, are characteristics PLA1 molecules family, member subfamily family that includes mPA-PLA1alpha...
We previously isolated a cDNA clone for galactosylceramide expression factor 1, which is the rat homologue of hepatocyte-growth-factor-regulated tyrosine kinase substrate (HGS) and induces morphological changes in COS-7 cells, reported that overexpression HGS induced canine kidney epithelial MDCK cells. component endosomal sorting complexes required transport machinery mediates multivesicle body formation. In this study, epithelial–mesenchymal transition caused transformation whereas...
The complete amino acid sequence of a white kidney bean (Phaseolus vulgaris) alpha-amylase inhibitor (PHA-I), which is composed two kinds glycopolypeptide subunits, alpha and beta, was established by conventional methods. polypeptide molecular weight PHA-I determined the light-scattering technique, considered together with weights revealed for indicated that has subunit stoichiometry (alpha beta)2 complex. Inhibition test increasing amounts porcine pancreatic (PPA) suggested an inactive 2:1...
The association of gangliosides with specific proteins in the central nervous system was examined by co-immunoprecipitation an anti-ganglioside antibody. monoclonal antibody to ganglioside GD3 immunoprecipitated phosphoproteins 40, 53, 56, and 80 kDa from rat cerebellum. Of these proteins, 40-kDa protein identified as α-subunit a heterotrimeric G protein, Go (Gαo). Using sucrose density gradient analysis cerebellar membranes, Gαo, but not Gβγ, observed detergent-resistant membrane (DRM) raft...
The association of gangliosides with specific proteins in the central nervous system was examined by coimmunoprecipitation an anti-ganglioside antibody. monoclonal antibody to ganglioside GD3 (R24) immunoprecipitated Csk (C-terminal src kinase)-binding protein (Cbp). Sucrose density gradient analysis showed that Cbp rat cerebellum detected detergent-resistant membrane (DRM) raft fractions. R24 treatment primary cerebellar cultures induced Lyn activation and tyrosine phosphorylation Cbp....
Stromal cell-derived factor-1α (SDF-1α)-induced platelet aggregation is mediated through its G protein-coupled receptor CXCR4 and phosphatidylinositol 3 kinase (PI3K). Here, we demonstrate that SDF-1α induces phosphorylation of Akt at Thr308 Ser473 in human platelets. SDF-1α-induced are inhibited by pretreatment with the antagonist AMD3100 or PI3K inhibitor LY294002. also PDK1 Ser241 (an upstream activator Akt), GSK3β Ser9 (a downstream substrate myosin light chain Ser19 element signaling...
Protein hyper- or hypophosphorylation induced by okadaic acid (OA) treatment was examined using quiescent cultures of the BALB/MK-2 mouse epidermal keratinocytes. Treatment with OA enhanced phosphorylation five proteins molecular weights 65,000, 55,000, 50,000, 28,000 and 15,000 (p65, p55, p50, p28, p15, respectively) decreased that two 22,000 20,000 (p22 p20, respectively). The major phosphorylated proteins, p65 were identified as type II I keratins, respectively, immunoblotting...
A collagen-binding glycoprotein was isolated from human platelets using affinity chromatography of immobilized collagen.Based upon characterizations this protein we confirmed that it identical to the propolypeptide von Willebrand factor (pp-vWF), which is also called antigen 11.The characteristics have investigated are molecular weight, existence carbohydrate chains, and NH2terminal amino acid sequence.pp-vWF has strong collagen inhibits collagen-induced aggregation at a concentration as low...