Abstract The precise regulation of the activity Cas9 is crucial for safe and efficient editing. Here we show that genome-editing can be constrained by addition cytosine stretches to 5′-end conventional single-guide RNAs (sgRNAs). Such a ‘safeguard sgRNA’ strategy, which compatible with Cas12a systems gene activation interference via CRISPR (clustered regularly interspaced short palindromic repeats), leads length-dependent inhibition formation functional complexes. Short extensions reduced...

Cerebral venous ischemia often induces severe brain edema. Vascular endothelial growth factor (VEGF), which angiogenesis, is also known as vascular permeability (VP) factor. The present study was undertaken to investigate whether the inhibition of VEGF could reduce edema formation and cerebral infarction (CVI) in a rat 2-vein occlusion (2-VO) model.We used 2-VO model 2 adjacent cortical veins were photochemically occluded. Male Wistar rats (n=25) divided into groups: one group treated with...

Background: Orthostatic hypotension can occur during acute spinal cord injury (SCI) and subsequently persist. We investigated whether a gait rehabilitation robot combined with functional electrical stimulation (FES) stabilizes hemodynamics orthostatic stress in SCI. Methods: Six intermediate-phase SCI patients (five males one female; mean age: 49.5 years; four quadriplegia two paraplegia) participated. The participants underwent robotic training (RT), FES, tilt table (TT). Hemodynamics were...

Abstract Background Glutamate decarboxylase 1 (GAD1), a rate-limiting enzyme in the production of γ-aminobutyric acid (GABA), is found GABAergic neurons central nervous system. Little known about relevance GAD1 to oral squamous cell carcinoma (OSCC). We investigated expression status and its functional mechanisms OSCCs. Methods evaluated mRNA protein expressions OSCC-derived cells using real-time quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) immunoblotting analyses....

LIM and SH3 protein 1 (LASP-1) is a specific focal adhesion involved in several malignant tumors. However, its role oral squamous cell carcinoma (OSCC) unknown. The aim of this study was to characterize the molecular status/mechanism LASP-1 OSCC.We evaluated mRNA expressions OSCC-derived lines primary OSCCs. Using an shRNA system, we analyzed effect on biology function OSCC lines, HSC-3 Ca9-22. cells also were subcutaneously injected evaluate tumor growth vivo. Data by Fisher's exact test or...

A DNA aptamer binding to the environment-sensitive fluorophore, dapoxyl, which increased fluorescence upon binding, was selected and used construct sensors by fusion with analyte-binding aptamers.

Collagen XVIII is a component of the highly specialized extracellular matrix associated with basement membranes epithelia and endothelia. In normal kidney, collagen distributed throughout glomerular tubular membranes, mesangial matrix, Bowman's capsule. Proteolytic cleavage within its C-terminal domain releases fragment endostatin, which has antiangiogenic properties. Because damage to membrane (GBM) accompanies immune-mediated renal injury, we investigated role XVIII/endostatin in this...

Protein O-fucosyltransferase 1 (POFUT1) is the enzyme that adds O-fucose through O-glycosidic linkage to conserved serine or threonine residues in epidermal growth factor-like repeats of a number cellular surface and secreted proteins. Our previous study using microarray technology showed significant upregulation POFUT1 occurs oral squamous cell carcinoma (OSCC)-derived lines compared human normal keratinocytes. The aim present was examine status mRNA protein expression OSCC-derived primary...

Inorganic/organic hybrid ultrathin films of MoS2 and a cationic amphiphile, dihexadecyldimethylammonium bromide (DHA+Br-), were prepared using the Langmuir−Blodgett (LB) technique. The surface pressure−area isotherms DHA+Br- changed by introducing exfoliated particles into subphase. On other hand, icosanoic acid in presence absence subphase essentially same. UV/vis reflection spectra DHA+(Br-) monolayers on suspension showed broad absorption band assignable to MoS2, whereas that did not show...

We have studied the thermal structural stability of liposomes encapsulating proteins by using synchrotron radiation small- and wide-angle X-ray scattering (SR-SWAXS). Liposomes are known to be effective drug-delivery systems (DDSs) because they can reduce drug toxicity due biodegradability biocompatibility offer promising carriers various types drugs. However, in spite numerous studies liposomes, physicochemical characteristics entrapping rarely known. The liposome is characterized lipid...

Abstract Objective The aim of this study was to evaluate objective limb function using force plate gait analysis after tibial plateau levelling osteotomy (TPLO) in small breed dogs with cranial cruciate ligament rupture (CCLR). Materials and Methods Small (15 kg or less) unilateral CCLR treated TPLO were evaluated symmetry index (SI) peak vertical (PVF), impulse vector magnitude at PVF performed preoperatively 1, 2, 4 6 months postoperatively routine surgery. Results Twelve met the inclusion...

The purpose of this study was to evaluate the association between early stages lumbar spinal stenosis (LSS) and risk locomotive syndrome, as well its effect upon muscle strength back, upper extremities, lower extremities.LSS diagnosed with a self-administered, self-reported history questionnaire. Participants (n=113) who agreed be tested by diagnostic support tool for LSS underwent three tests syndrome: stand-up test, two-step 25-question Geriatric Locomotive Function Scale (GLFS-25),...

Introduction. An arachnoid web (AW) is a relatively rare disease and shows clinical symptoms radiological findings similar to those of an cyst (AC) or spinal cord herniation (SCH). Since the operative procedures for AW are generally different from intrathecal disorders, correct preoperative differential diagnosis important. The purposes this study were report usefulness magnetic resonance imaging (MRI) computed tomography (CT) myelography diagnosing show histological results. Case...

We have developed a robot for gait rehabilitation of paraplegics use in combination with functional electrical stimulation (FES). The purpose this study was to verify whether the robot-derived torque can be reduced by using FES healthy-person pseudo-paraplegic model.Nine healthy participants (22-36 years old) participated study. exoskeleton designed based on hip-knee-ankle-foot orthosis paraplegia. Participants walked treadmill lift support their weight. bilateral quadriceps femoris and...

The purpose of this study was to investigate the relationship between clinical outcomes and lateral thrust before after unicompartmental knee arthroplasty (UKA) using inertial measurement sensor units. Eleven knees were evaluated with gait analysis. varus angular velocity used evaluate thrust. femoro-tibial angle (FTA) hip-knee-ankle (HKA) lower limb alignment, Oxford Knee Score (OKS) Japanese Orthopaedic Association (JOA) outcomes. mean pre-UKA peak 37.1 ± 9.8°/sec, that post-UKA 28.8...

Self-assembled monolayers (SAMs) of heavy chalcogenophenes and dialkyl chalcogenides on Au(111) were prepared by a vapor adsorption method. The chalcogenide analogues was confirmed X-ray photoelectron spectroscopy. structures the films observed using scanning tunneling microscopy under atmospheric conditions. SAM selenophene stable showed striped patterns with row-to-row spacing 16 ± 1 Å. Dibutyl selenide also formed but longer exposure times. Tellurophene dibutyl telluride found to adsorb...

Aristolochic acid (AA) nephropathy, first reported as Chinese herbs is a rapidly progressive tubulointerstitial nephropathy that results in severe anemia, interstitial fibrosis and end-stage renal disease. Tubulointerstitial injury was studied mouse model of AA to determine whether low-dose darbepoetin alpha (DPO) treatment prevents acute tubular necrosis fibrosis.AA administered C3H/He mice intraperitoneally some were also treated with 0.1 microg/kg DPO weekly starting on the day...

Lipid liposomes are promising drug delivery systems because they have superior curative effects owing to their high adaptability a living body. encapsulating proteins were constructed and the structures examined using synchrotron radiation small- wide-angle X-ray scattering (SR-SWAXS). The prepared by sequential combination of natural swelling, ultrasonic dispersion, freeze-throw, extrusion spin-filtration. composed acidic glycosphingolipid (ganglioside), cholesterol phospholipids. By...