A5042850369- Glaucoma and retinal disorders
- Retinal Diseases and Treatments
- Retinal Development and Disorders
- Intraocular Surgery and Lenses
- Corneal surgery and disorders
- Gamma-ray bursts and supernovae
- Ocular Surface and Contact Lens
- Retinal Imaging and Analysis
- Astronomical Observations and Instrumentation
- Mitochondrial Function and Pathology
- Ubiquitin and proteasome pathways
- Ophthalmology and Visual Impairment Studies
- Retinal and Macular Surgery
- Astrophysical Phenomena and Observations
- Axon Guidance and Neuronal Signaling
- SAS software applications and methods
- Retinopathy of Prematurity Studies
- Retinal and Optic Conditions
- Nerve injury and regeneration
- Photoreceptor and optogenetics research
- Astronomy and Astrophysical Research
- Neurogenesis and neuroplasticity mechanisms
- Chemical Synthesis and Characterization
- Astrophysics and Cosmic Phenomena
- Glycosylation and Glycoproteins Research
Kobe City Medical Center General Hospital
2022-2025
RIKEN Center for Biosystems Dynamics Research
2021-2024
Smith-Kettlewell Eye Research Institute
2019-2023
Stanford University
2019-2023
Vision Eye Institute
2022
Kyoto University
1996-2019
Nidek (Japan)
2013-2019
Nagoya Institute of Technology
2016-2018
Satou Hospital
2018
Google (United States)
2018
Heat shock protein 47 (HSP47), a collagen-specific stress protein, has been postulated to be molecular chaperone localized in the ER. We previously demonstrated that HSP47 transiently associated with newly synthesized procollagen ER (Nakai, A., M. Satoh, K. Hirayoshi, and Nagata. 1992. J. Cell Biol. 117:903-914). In present work, we examined location where binds dissociates from within cells, whether associates nascent single polypeptide chains and/or mature triple-helix procollagen. This...
A 47-kDa heat shock protein (HSP47) is a collagenbinding stress which localized in the endoplasmic reticulum (ER) of collagen-secreting cells.Recent studies have shown that HSP47 transiently binds to newly synthesized procollagens and conformationally abnormal procollagen also bound by for much longer time ER (Nakai, A, Satoh, M., Hirayoshi, K., Nagata, K. (1992) J. Cell Biol.117,[903][904][905][906][907][908][909][910][911][912][913][914].HSP47 thus suggested collagenspecific molecular...
Background: Retinal and optic disc perfusion in nonarteritic anterior ischemic neuropathy (NAION) is incompletely understood. Our aim was to investigate the characteristics of microvascular structures at peripapillary area disc, their associations with retinal structure function patients NAION. Methods: We conducted a prospective, observational case series study. Thirty-four eyes, consisting 15 NAION eyes 19 normal were included. Optical coherence tomography (OCT) angiography used measure...
5-Fluorouracil (5-FU) chemotherapy is the first choice treatment for advanced hepatocellular carcinoma (HCC), and resistance major obstacle to successful treatment. Recent studies have reported that epithelial-to-mesenchymal transition (EMT) associated with chemoresistance in cancers. We speculated EMT 5-FU metabolism are related mechanism of resistance. First, two 5-FU-resistant cell lines, HLF-R4 HLF-R10, were established from HLF undifferentiated human HCC line. Whereas growth was similar...
Many neurons in the adult central nervous system, including retinal ganglion cells (RGCs), degenerate and die after injury. Early axon protein organelle trafficking failure is a key component many neurodegenerative disorders yet changes to axoplasmic transport disease models have not been quantified. We analyzed early ‘transportome’ from RGC somas their axons optic nerve injury identified of an anterograde motor Kif5a degeneration. demonstrated that manipulating expression affects...
Glaucoma, the number one cause of irreversible blindness, is characterized by loss retinal ganglion cells (RGCs), which do not regenerate in humans or mammals after cell death. Cell transplantation provides an opportunity to restore vision glaucoma, other optic neuropathies. Since transplanting primary RGCs from deceased donor tissues may be feasible, stem cell-derived could provide a plausible alternative source for transplant.We define robust chemically defined protocol differentiate human...
LIM and SH3 protein 1 (LASP-1) is a specific focal adhesion involved in several malignant tumors. However, its role oral squamous cell carcinoma (OSCC) unknown. The aim of this study was to characterize the molecular status/mechanism LASP-1 OSCC.We evaluated mRNA expressions OSCC-derived lines primary OSCCs. Using an shRNA system, we analyzed effect on biology function OSCC lines, HSC-3 Ca9-22. cells also were subcutaneously injected evaluate tumor growth vivo. Data by Fisher's exact test or...
<h3>Abstract</h3> The failure of adult CNS neurons to survive and regenerate their axons after injury or in neurodegenerative disease remains a major target for basic clinical neuroscience. Recent data demonstrated the mouse that exogenous expression Sry-related high-mobility-box 11 (Sox11) promotes optic nerve regeneration but exacerbates death subset retinal ganglion cells (RGCs), α-RGCs. During development, Sox11 is required RGC differentiation from progenitor (RPCs), we found mutation...
Objective Cystoid macular edema (CME) in retinitis pigmentosa (RP) is an important complication causing visual dysfunction. We investigated the effect of CME on photoreceptors RP patients with previous or current CME, using adaptive optics (AO) fundus camera. Methods retrospectively observed and ellipsoid zone (EZ) length (average horizontal vertical sections) by optical coherence tomography. The density regularity arrangement photoreceptor cells (Voronoi analysis) were examined at four...
Protein O-fucosyltransferase 1 (POFUT1) is the enzyme that adds O-fucose through O-glycosidic linkage to conserved serine or threonine residues in epidermal growth factor-like repeats of a number cellular surface and secreted proteins. Our previous study using microarray technology showed significant upregulation POFUT1 occurs oral squamous cell carcinoma (OSCC)-derived lines compared human normal keratinocytes. The aim present was examine status mRNA protein expression OSCC-derived primary...
Persephin (PSPN) is a neurotrophic factor of the glial cell line-derived (GDNF) family that promotes survival multiple populations neurons. Little known about relevance PSPN in human malignancy including oral squamous carcinoma (OSCC). This study was undertaken to evaluate mRNA and protein expression by analyzing cellular proliferation cycle knockdown cells vitro. were significantly (P < 0.05) up-regulated OSCC-derived compared with normal keratinocytes (n = 7). Cellular decreased reduced...
Adult central nervous system (CNS) axons fail to regenerate after injury, and master regulators of the regenerative program remain be identified. We analyzed transcriptomes retinal ganglion cells (RGCs) at 1 5 days optic nerve injury with without a cocktail strongly pro-regenerative factors discover genes that regulate survival regeneration. used advanced bioinformatic analysis identify top transcriptional upstream cross-referenced these differentially expressed between embryonic RGCs...
Purpose: Axon transport of organelles and neurotrophic factors is necessary for maintaining cellular function survival retinal ganglion cells (RGCs). However, it not clear how trafficking mitochondria, essential RGC growth maturation, changes during development. The purpose this study was to understand the dynamics regulation mitochondrial maturation using acutely purified RGCs as a model system. Methods: Primary were immunopanned from rats either sex three stages MitoTracker dye live-cell...
To determine the vitreous and aqueous concentrations of brimonidine after topical application ophthalmic solution 0.1%.The prospective observational case series included patients with an idiopathic epiretinal membrane or macular hole who were scheduled for a pars plana vitrectomy. Brimonidine tartrate 0.1% was topically administered twice daily 1 week preoperatively. Vitreous humor collected before vitrectomy, then, concentration measured liquid chromatography tandem spectrometry...
Purpose To use adaptive optics scanning laser ophthalmoscopy (AO-SLO) to quantify cone loss ratio in the foveola order assess foveal status and investigate relationships between structural abnormalities visual function patients with macular hole (MH) after surgery. Methods We evaluated 10 normal eyes of healthy volunteers 19 18 whom anatomically successful MH closure had been performed. All subjects underwent a comprehensive ophthalmologic examination that included measurements...
Abstract Several clinical studies have been conducted into the practicality and safety of regenerative therapy using hESC/iPSC-retinal pigment epithelium (RPE) as a treatment for diseases including age-related macular degeneration. These used either suspensions RPE cells or an cell sheet. The can be injected minimally invasive procedure but delivery intended number at exact target location is difficult; sheets take longer time to prepare, surgical placed area. In research reported here, we...
Purpose: We aimed to explore the potential benefits of retinal pigment epithelium (RPE) replacement therapy in patients with Bietti crystalline dystrophy (BCD) by assessing disease pathology distinctive relationship between fundus autofluorescence (FAF) abnormality and visual field defect (VFD). Methods: Sixteen eyes from 16 BCD RHO -associated retinitis pigmentosa ( -RP) were included. FAF, optical coherence tomography, Goldmann perimetry results retrospectively reviewed assessed using...
Abstract Clinical studies using suspensions or sheets of human pluripotent cell-derived retinal pigment epithelial cells (hiPSC-RPE) have been conducted globally for diseases such as age-related macular degeneration. Despite being minimally invasive, cell suspension transplantation faces challenges in targeted delivery and frequent leakage. Conversely, although the RPE sheet ensures with correct polarity, it requires invasive surgery, graft preparation is time-consuming. We previously...
Abstract We report on a Suzaku observation of the nearby Seyfert2 galaxy NGC4388. The overall spectrum is well described by combination strongly absorbed power-law plus thin thermal plasma emission, as previous observations. Thanks to well-calibrated instruments and low background level, measured broad-band X-ray spectra more precisely than ever, enabled us probe physical structure nuclear region. In addition, time variability having factor 1.5 with half-day scale was found up above 10keV....
This study aimed to evaluate the distribution of retinal pigment epithelium (RPE) melanin in patients with retinitis pigmentosa (RP) using entropy measurements by custom-made polarization-sensitive optical coherence tomography (PS-OCT) images, and compare intensity short-wavelength (SW) near-infrared (NIR) autofluorescence (AF). We retrospectively reviewed including PS-OCT, SW-AF, NIR-AF RP who had a hyperautofluorescent ring on AF. A total 12 eyes (8 women 4 men; mean age: 37.9 years) were...