A5037281531- Retinal Development and Disorders
- Glaucoma and retinal disorders
- Neuroscience and Neural Engineering
- Catalytic Processes in Materials Science
- Photoreceptor and optogenetics research
- Nanomaterials for catalytic reactions
- Advanced Photocatalysis Techniques
- Retinopathy of Prematurity Studies
- Retinal Diseases and Treatments
- Retinal and Macular Surgery
- Metal-Organic Frameworks: Synthesis and Applications
- Distributed Control Multi-Agent Systems
- Ocular Surface and Contact Lens
- Nerve injury and regeneration
- Renal and related cancers
- Pluripotent Stem Cells Research
- Advanced Memory and Neural Computing
- Cell death mechanisms and regulation
- Multimodal Machine Learning Applications
- Security in Wireless Sensor Networks
- Age of Information Optimization
- Calcium Carbonate Crystallization and Inhibition
- Neural Networks Stability and Synchronization
- Biocrusts and Microbial Ecology
- Pickering emulsions and particle stabilization
Stanford University
2020-2024
Sun Yat-sen University
2017-2024
China Electronics Technology Group Corporation
2021-2024
Smith-Kettlewell Eye Research Institute
2022-2024
Vision Eye Institute
2024
Lanzhou University of Technology
2023
Weatherford College
2023
Zhongshan Ophthalmic Center, Sun Yat-sen University
2017-2018
StemCells (United States)
2018
Harbin Engineering University
2015
Retinal ganglion cell (RGC) replacement therapy could restore vision in glaucoma and other optic neuropathies. We developed a rapid protocol for directly induced RGC (iRGC) differentiation from human stem cells, leveraging overexpression of NGN2. Neuronal morphology neurite growth were observed within 1 week induction; characteristic RGC-specific gene expression confirmed identity. Calcium imaging demonstrated γ-aminobutyric acid (GABA)-induced excitation immature RGCs. Single-cell RNA...
Abstract Effective derivation of three-dimensional (3D) retinal tissue from human-induced pluripotent stem cells (hiPSCs) could provide models for drug screening and facilitate patient-specific cell replacement therapy. However, some hiPSC lines cannot undergo 3D self-organization show inadequate differentiation efficiency to meet clinical demand. In this study, we developed an optimized system tissue. We found that the Wnt signaling pathway antagonist Dickkopf-related protein 1 (DKK-1)...
Transplantation of stem cell-derived retinal neurons is a promising regenerative therapy for optic neuropathy. However, significant anatomic differences compromise its efficacy in large animal models. The present study describes the procedure and outcomes human-induced pluripotent cell (hiPSC)-derived sheet transplantation primate models using biodegradable materials. Stem organoids were seeded on polylactic-coglycolic acid (PLGA) scaffolds directed toward ganglion (RGC) fate. tissues showed...
Neuroprotection after injury or in neurodegenerative disease remains a major goal for basic and translational neuroscience. Retinal ganglion cells (RGCs), the projection neurons of eye, degenerate optic neuropathies axon injury, there are no clinical therapies to prevent their loss restore connectivity targets brain. Here we demonstrate profound neuroprotective effect exogenous expression various Ca 2+ /calmodulin-dependent protein kinase II (CaMKII) isoforms mice. A dramatic increase RGC...
Purpose: Axon transport of organelles and neurotrophic factors is necessary for maintaining cellular function survival retinal ganglion cells (RGCs). However, it not clear how trafficking mitochondria, essential RGC growth maturation, changes during development. The purpose this study was to understand the dynamics regulation mitochondrial maturation using acutely purified RGCs as a model system. Methods: Primary were immunopanned from rats either sex three stages MitoTracker dye live-cell...
This study was conducted to determine the dynamic Islet1 and Brn3 (POU4F) expression pattern in human fetal retina human-induced pluripotent stem cell- (hiPSC-) derived retinal organoid. Human eyes from 8 27 weeks (Fwks), adult retina, hiPSC-derived organoid 7 31 differentiation (Dwks), rhesus were collected for cyrosectioning. Immunofluorescence analysis showed that expressed ganglion cells organoids. Unexpectedly, after Fwk 20, gradually decreased retina. In midstage of development,...
Objectives. Primary cilia are conserved organelles found in polarized mammalian cells that regulate neuronal growth, migration, and differentiation. Proper formation is essential during eye development. Our previous reports both amacrine retinal ganglion (RGCs) contain primary primate rodent retinas. However, whether present the inner retina of human organoids remains unknown. The purpose this study to characterize distribution embryonic stem cell (hESC-derived organoid Materials Methods....
With the great success of graphene in biomedical field, carbon nanotubes have attracted increasing attention for different applications ophthalmology. Here, we report a novel retinal sheet composed (CNTs) and poly(lactic-co-glycolic acid) (PLGA) that can enhance cell therapy. By tuning our CNTs to regulate mechanical characteristics retina sheets, were able improve vitro viability ganglion cells derived from human-induced pluripotent stem incorporated into CNTs. Engrafted displayed signs...
BAM15 is a novel mitochondrial protonophore uncoupler capable of protecting mammals from acute renal ischemic-reperfusion injury and cold-induced microtubule damage. The purpose our study was to investigate the effect on apoptosis during 5-day transportation human-induced pluripotent stem (hiPS)-differentiated retinal tissue. Retinal tissues 30 days 60 were transported with or without for 5 in laboratory by real express. Immunofluorescence staining marker cleaved caspase3, proliferation...
Mammalian central nervous system (CNS) axons cannot spontaneously regenerate after injury, creating an unmet need to identify molecular regulators promote axon regeneration and reduce the lasting impact of CNS injuries. While tubulin polymerization promoting protein family member 3 (Tppp3) is known outgrowth in amphibians, its role mammalian remains unknown. Here we investigated Tppp3 retinal ganglion cells (RGCs) neuroprotection axonal using optic nerve crush (ONC) model rodent. Single-cell...
We investigated the efficacy of immunosuppressants rapamycin (RAP) and dexamethasone (DEX) in improving survival retinal organoids after epiretinal transplantation. first compared immunosuppressive abilities DEX RAP activated microglia an vitro setting. Following this, we used immunofluorescence, real-time polymerase chain reaction, flow cytometry to investigate effects on cells organoids. Retinal were then seeded onto poly(lactic-co-glycolic) acid (PLGA) scaffolds implanted into rhesus...
Background . To develop an effective surgical procedure for cellular scaffold epiretinal implantation in rhesus, facilitating subsequent stem cell transplantation. Methods Retinal progenitors were seeded onto a poly(lactic-co-glycolic) acid (PLGA) scaffold. First, the scaffolds delivered by 18G catheter or retinal forceps into rabbit space (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mi>n</mml:mi><mml:mo>=</mml:mo><mml:mn>50</mml:mn></mml:math>). Then, survival rate...
With nodes in Delay Tolerant Network(DTN) distributing sparsely and moving rapidly, they usually suffer from intermittent connections communications, thus bringing about limited message forwarding opportunities. All these could lead to inefficient forwarding, low delivery, long latency transmission capacity performance. In this paper, efficient encoding decision methods are presented integrated into the DTN routing strategy. The custody-encoding-forwarding mode is designed by merging random...