A5021421512- Retinal Development and Disorders
- Mitochondrial Function and Pathology
- Nerve injury and regeneration
- Ubiquitin and proteasome pathways
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Axon Guidance and Neuronal Signaling
- Cellular transport and secretion
- Hearing, Cochlea, Tinnitus, Genetics
- Endoplasmic Reticulum Stress and Disease
- Infection Control and Ventilation
- Neural dynamics and brain function
- Neuroscience and Neuropharmacology Research
- Neurological diseases and metabolism
- Retinopathy of Prematurity Studies
- Infant Development and Preterm Care
- Neuroinflammation and Neurodegeneration Mechanisms
- COVID-19 and healthcare impacts
- Animal Vocal Communication and Behavior
- S100 Proteins and Annexins
- Neurogenesis and neuroplasticity mechanisms
- Dental Anxiety and Anesthesia Techniques
- Emergency and Acute Care Studies
- Pharmaceutical studies and practices
- Neuroscience and Neural Engineering
Stanford University
2018-2025
University of South Florida
2024
Nova Southeastern University
2024
Smith-Kettlewell Eye Research Institute
2018-2024
Palm Springs General Hospital
2024
Lake Erie College of Osteopathic Medicine
2024
Larkin Community Hospital
2024
Community Hospital
2024
Dr. A. Ramachandran's Diabetes Hospitals
2024
Vision Eye Institute
2024
cAMP signaling is known to be critical in neuronal survival and axon growth. Increasingly the subcellular compartmentation of has been appreciated, but outside dendritic synaptic regulation, few compartments have defined terms molecular composition or function neurons. Specificity conferred large part by A-kinase anchoring proteins (AKAPs) that localize protein kinase A other enzymes discrete intracellular compartments. We now reveal within a perinuclear compartment organized multivalent...
The cars we drive, the homes live in, restaurants visit, and laboratories offices work in are all a part of modern human habitat. Remarkably, little is known about diversity chemicals present these environments to what degree molecules from our bodies influence built environment that surrounds us vice versa. We therefore set out visualize chemical five habitats together with their occupants, provide snapshot various which humans exposed on daily basis. molecular inventory was obtained...
Many neurons in the adult central nervous system, including retinal ganglion cells (RGCs), degenerate and die after injury. Early axon protein organelle trafficking failure is a key component many neurodegenerative disorders yet changes to axoplasmic transport disease models have not been quantified. We analyzed early ‘transportome’ from RGC somas their axons optic nerve injury identified of an anterograde motor Kif5a degeneration. demonstrated that manipulating expression affects...
Optineurin (OPTN) mutations are linked to amyotrophic lateral sclerosis (ALS) and normal tension glaucoma (NTG), but a relevant animal model is lacking, the molecular mechanisms underlying neurodegeneration unknown. We find that OPTN C-terminus truncation (OPTN∆C) causes late-onset of retinal ganglion cells (RGCs), optic nerve (ON), spinal cord motor neurons, preceded by decrease axonal mitochondria in mice. discover directly interacts with both microtubules mitochondrial transport complex...
Retinal ganglion cell (RGC) replacement therapy could restore vision in glaucoma and other optic neuropathies. We developed a rapid protocol for directly induced RGC (iRGC) differentiation from human stem cells, leveraging overexpression of NGN2. Neuronal morphology neurite growth were observed within 1 week induction; characteristic RGC-specific gene expression confirmed identity. Calcium imaging demonstrated γ-aminobutyric acid (GABA)-induced excitation immature RGCs. Single-cell RNA...
Abstract Blood vessels in the central nervous system (CNS) develop unique features, but contribution of CNS neurons to regulating those features is not fully understood. We report that inhibiting spontaneous cholinergic activity or reducing starburst amacrine cell numbers prevents invasion endothelial cells into deep layers retina and causes blood-retinal-barrier (BRB) dysfunction mice. Vascular growth factor (VEGF), which drives angiogenesis, Norrin, a Wnt ligand induces BRB properties, are...
Intercellular transfer of toxic proteins between neurons is thought to contribute neurodegenerative disease, but whether direct interneuronal protein occurs in the healthy brain not clear. To assess prevalence and identity transferred cellular specificity transfer, we biotinylated retinal ganglion cell vivo examined transported through rodent visual circuit using microscopy, biochemistry, mass spectrometry. Electron microscopy demonstrated preferential from retinogeniculate inputs excitatory...
Adult central nervous system (CNS) axons fail to regenerate after injury, and master regulators of the regenerative program remain be identified. We analyzed transcriptomes retinal ganglion cells (RGCs) at 1 5 days optic nerve injury with without a cocktail strongly pro-regenerative factors discover genes that regulate survival regeneration. used advanced bioinformatic analysis identify top transcriptional upstream cross-referenced these differentially expressed between embryonic RGCs...
Purpose: Axon transport of organelles and neurotrophic factors is necessary for maintaining cellular function survival retinal ganglion cells (RGCs). However, it not clear how trafficking mitochondria, essential RGC growth maturation, changes during development. The purpose this study was to understand the dynamics regulation mitochondrial maturation using acutely purified RGCs as a model system. Methods: Primary were immunopanned from rats either sex three stages MitoTracker dye live-cell...
Cross-linked protein-coated microcrystals (CLPCMCs) of subtilisin Carlsberg (SC), and lipases from Candida rugosa (CRL) Burkholderia cepacia (BCL) were prepared by cross-linking (PCMCs). These showed enhanced reaction rates for transesterification in organic solvents different polarity compared with PCMCs. CLPCMCs SC catalytically active up to 80°C octane gave 98% conversion 6 h, while PCMCs only 58% 8 h conversions did not further increase time. The Fourier transform infrared spectra that...
Axons from the nucleus magnocellularis form a presynaptic map of interaural time differences (ITDs) in laminaris (NL). These inputs generate field potential that varies systematically with recording position and can be used to measure ITDs. In barn owl, representation best ITD shifts mediolateral NL, so as continuous, smoothly overlapping maps iso-ITD contours are not parallel NL border. Frontal space (0°) is, however, represented throughout thus overrepresented respect periphery....
Retinal ganglion cells (RGCs) die after optic nerve trauma or in degenerative disease. However, acute changes protein expression that may regulate RGC response to injury are not fully understood, and detailed methods quantify new synthesis have been tested. Here, we develop apply a vivo quantitative measure of newly synthesized proteins examine occurring the retina injury. Azidohomoalanine, noncanonical amino acid, was injected intravitreally into eyes rodents either sex with without Isotope...
The brain processes information and generates cognitive motor outputs through functions of spatially organized proteins in different types neurons. More complete knowledge their distributions within neuronal compartments intact circuits would help the understanding function. We used unbiased vivo protein labeling with intravitreal NHS-biotin for discovery analysis endogenous axonally transported visual system using tandem mass spectrometric proteomics, biochemistry, both light electron...
Inputs from the two sides of brain interact to create maps interaural time difference (ITD) in nucleus laminaris birds. How inputs each side are matched with high temporal precision ITD-sensitive circuits is unknown, given differences input path lengths side. To understand this problem birds, we modeled geometry axons and their corresponding conduction velocities latencies. Consistent existing physiological data, assumed a common latency up border laminaris. We analyzed biological...
Various breathing and cough simulators have been used to model respiratory droplet dispersion viral droplets, in particular for SARS-CoV-2 modeling. However, limited data are available comparing these simulations physiological coughing. In this study, three different (Teleflex Mucosal Atomization Device Nasal (MAD Nasal), a spray gun, GloGermTM MIST) that the literature were studied assess their physiologic relevance. Droplet size, velocity, dispersion, force generated by measured. size was...
Abstract Optineurin (OPTN) mutations are linked to amyotrophic lateral sclerosis (ALS) and normal tension glaucoma (NTG), but a relevant animal model is lacking, the molecular mechanisms underlying neurodegeneration unknown. We found that OPTN C-terminus truncation (OPTNΔC) causes late-onset of retinal ganglion cells (RGCs), optic nerve (ON), spinal cord motor neurons, preceded by striking decrease axonal mitochondria. Surprisingly, we discover directly interacts with both microtubules...
Neural progenitor cells lengthen their cell cycle to prime themselves for differentiation as development proceeds. It is currently not clear how they counter this lengthening and avoid being halted in the cycle. We show that N
Workplace violence is a significant issue among nurses, particularly in tertiary care hospitals, impacting their well-being and job performance. Understanding the prevalence types of can help develop effective strategies to mitigate these incidents. Objective: To investigate prevalence, types, frequency, impact workplace nurses hospitals Lahore. Methods: This descriptive cross-sectional study was conducted various Lahore over six-month period following approval. The population comprised...
To assess the views of paediatric specialist dental practitioners in United Kingdom use hand over mouth technique and physical restraint.Questionnaire survey all dentistry (n = 216). Replies were received from 179 individuals (82.8%).The majority sample (over 80%) described HOM as having three components, broadly mirroring description clinical textbooks. Approximately 60% respondents reported that should never be used (106 individuals, 59.2%). Those who endorsed suggested it with cases...