A5081661991- Retinal Development and Disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Animal Virus Infections Studies
- Cancer-related molecular mechanisms research
- Single-cell and spatial transcriptomics
- Viral gastroenteritis research and epidemiology
- Retinal Diseases and Treatments
- Neurogenesis and neuroplasticity mechanisms
- interferon and immune responses
- Glaucoma and retinal disorders
- Viral Infections and Immunology Research
- Cardiac Ischemia and Reperfusion
- Sirtuins and Resveratrol in Medicine
- Autophagy in Disease and Therapy
- Olfactory and Sensory Function Studies
- Nerve injury and regeneration
- Corneal surgery and disorders
- Photoreceptor and optogenetics research
- Adipose Tissue and Metabolism
- MicroRNA in disease regulation
- Anesthesia and Neurotoxicity Research
- Adipokines, Inflammation, and Metabolic Diseases
- Plant Virus Research Studies
- Virus-based gene therapy research
- Cardiovascular Function and Risk Factors
Harvard University
2018-2025
Xijing Hospital
2013-2025
Sichuan University
2020-2025
Air Force Medical University
2011-2025
Ministry of Education of the People's Republic of China
2024
Stanford University
2023-2024
Affiliated Eye Hospital of Wenzhou Medical College
2022-2023
Wenzhou Medical University
2022-2023
Chinese Academy of Medical Sciences & Peking Union Medical College
2023
Center for Pain and the Brain
2018-2023
Amacrine cells (ACs) are a diverse class of interneurons that modulate input from photoreceptors to retinal ganglion (RGCs), rendering each RGC type selectively sensitive particular visual features, which then relayed the brain. While many AC types have been identified morphologically and physiologically, they not comprehensively classified or molecularly characterized. We used high-throughput single-cell RNA sequencing profile >32,000 ACs mice both sexes applied computational methods...
Abstract Most irreversible blindness results from retinal disease. To advance our understanding of the etiology blinding diseases, we used single-cell RNA-sequencing (scRNA-seq) to analyze transcriptomes ~85,000 cells fovea and peripheral retina seven adult human donors. Utilizing computational methods, identified 58 cell types within 6 classes: photoreceptor, horizontal, bipolar, amacrine, ganglion non-neuronal cells. Nearly all are shared between two regions, but there notable differences...
Cognitive processes require working memory (WM) that involves a brief period of retention known as the delay period. Elevated delay-period activity in medial prefrontal cortex (mPFC) has been observed, but its functional role WM tasks remains unclear. We optogenetically suppressed or enhanced pyramidal neurons mouse mPFC during Behavioral performance was impaired learning phase not after mice were well trained. Delay-period appeared to be more important than inhibitory control,...
Increased intraocular pressure (IOP) represents a major risk factor for glaucoma, prevalent eye disease characterized by death of retinal ganglion cells; lowering IOP is the only proven treatment strategy to delay progression. The main determinant equilibrium between production and drainage aqueous humor, with compromised generally viewed as primary contributor dangerous elevations. Drainage occurs through two pathways in anterior segment called conventional uveoscleral. To gain insights...
Retinal structure and function have been studied in many vertebrate orders, but molecular characterization has largely confined to mammals. We used single-cell RNA sequencing (scRNA-seq) generate a cell atlas of the chick retina. identified 136 types plus 14 positional or developmental intermediates distributed among six classes conserved across vertebrates – photoreceptor, horizontal, bipolar, amacrine, retinal ganglion, glial cells. To assess morphology molecularly defined types, we...
The anterior segment of the eye consists cornea, iris, ciliary body, crystalline lens, and aqueous humor outflow pathways. Together, these tissues are essential for proper functioning eye. Disorders vision have been ascribed to defects in all them; some disorders, including glaucoma cataract, among most prevalent causes blindness world. To characterize cell types that compose tissues, we generated an atlas human using high-throughput single-nucleus RNA sequencing (snRNAseq). We profiled...
Abstract The basic plan of the retina is conserved across vertebrates, yet species differ profoundly in their visual needs 1 . Retinal cell types may have evolved to accommodate these varied needs, but this has not been systematically studied. Here we generated and integrated single-cell transcriptomic atlases from 17 species: humans, two non-human primates, four rodents, three ungulates, opossum, ferret, tree shrew, a bird, reptile, teleost fish lamprey. We found high molecular conservation...
Primary open-angle glaucoma (POAG), characterized by retinal ganglion cell death, is a leading cause of irreversible blindness worldwide. However, its molecular and cellular causes are not well understood. Elevated intraocular pressure (IOP) major risk factor, but many patients have normal IOP. Colocalization Mendelian randomization analysis >240 POAG IOP genome-wide association study (GWAS) loci overlapping expression splicing quantitative trait (e/sQTLs) in 49 GTEx tissues retina...
Cardiac metabolic remodeling is a central event during heart failure (HF) development following myocardial infarction (MI). It well known that glucose and fatty acid dysmetabolism contribute to post-MI cardiac dysfunction remodeling. However, the role of amino metabolism in HF remains elusive. Branched chain acids (BCAAs) are an important group essential function as crucial nutrient signaling mammalian animals. The present study aimed determine BCAA progression. Utilizing coronary artery...
Our previous studies have shown that hyperbaric oxygen preconditioning (HBO-PC) induces tolerance to cerebral ischemia/reperfusion (I/R). This study aimed investigate whether SirT1, a class III histone deacetylase, is involved in neuroprotection elicited by HBO-PC animal and cell culture models of ischemia. Rats were subjected middle artery occlusion for 120 minutes after (once day 5 days). Primary cultured cortical neurons exposed 2 hours oxygen–glucose deprivation (OGD). We showed...
Salmonella Enteritidis (S. Enteritidis) being one of the most prevalent foodborne pathogens worldwide poses a serious threat to public safety. Prevention zoonotic infectious disease and controlling risk transmission S. Enteriditidis critically requires evolution rapid sensitive detection methods. The methods based on nucleic acid conventional antibodies are fraught with limitations. Many these limitations can be circumvented using natural nanobodies which endowed characteristics, such as...
Vertebrate retinas share a basic blueprint comprising 5 neuronal classes arranged according to common wiring diagram. Yet, vision is aligned with species differences in behavior and ecology, raising the question of how evolution acts on this circuit adjust its computational characteristics. We address that problem by comparing thalamic visual code retinal cell composition closely related occupying different niches: Rhabdomys pumilio, which are day-active murid rodents, nocturnal laboratory...
Human guanylate-binding protein 1 (hGBP1) is an interferon-inducible involved in the host immune response against viral infection. In to infection by influenza A virus (IAV), hGBP1 transcript and were significantly upregulated. Overexpression of inhibited IAV replication a dose-dependent manner vitro. The lysine residue at position 51 (K51) was essential for inhibition replication. Mutation K51 resulted that unable inhibit nonstructural (NS1) found interact directly with hGBP1. region...
The basic plan of the retina is conserved across vertebrates, yet species differ profoundly in their visual needs (Baden et al., 2020). One might expect that retinal cell types evolved to accommodate these varied needs, but this has not been systematically studied. Here, we generated and integrated single-cell transcriptomic atlases from 17 species: humans, two non-human primates, four rodents, three ungulates, opossum, ferret, tree shrew, a teleost fish, bird, reptile lamprey. Molecular...
Although the visual system extends through brain, most vision loss originates from defects in eye. Its central element is neural retina, which senses light, processes signals, and transmits them to rest of brain optic nerve (ON). Surrounding retina are numerous other structures, conventionally divided into anterior posterior segments. Here, we used high-throughput single-nucleus RNA sequencing (snRNA-seq) classify characterize cells six extraretinal components segment: ON, head (ONH),...