A5023611360- Glaucoma and retinal disorders
- Retinal Diseases and Treatments
- Intraocular Surgery and Lenses
- Retinal Development and Disorders
- Traumatic Ocular and Foreign Body Injuries
- Ocular Infections and Treatments
- Corneal surgery and disorders
- Retinal and Optic Conditions
- Ocular Diseases and Behçet’s Syndrome
- Corneal Surgery and Treatments
- Herpesvirus Infections and Treatments
- Retinal Imaging and Analysis
- Cancer-related molecular mechanisms research
- Virus-based gene therapy research
- Facial Trauma and Fracture Management
- Restraint-Related Deaths
- Pneumocystis jirovecii pneumonia detection and treatment
- Cerebral Venous Sinus Thrombosis
- Neuroinflammation and Neurodegeneration Mechanisms
- Cell Adhesion Molecules Research
- Lipid metabolism and disorders
- Rabies epidemiology and control
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Ophthalmology and Visual Impairment Studies
- Retinal and Macular Surgery
Regeneron (United States)
2021-2025
Yale University
2012-2024
Harvard University
2015-2024
Massachusetts Eye and Ear Infirmary
2015-2023
Joint Base San Antonio
2021
Brooke Army Medical Center
2021
Cornea Associates of Texas
2021
Abstract Most irreversible blindness results from retinal disease. To advance our understanding of the etiology blinding diseases, we used single-cell RNA-sequencing (scRNA-seq) to analyze transcriptomes ~85,000 cells fovea and peripheral retina seven adult human donors. Utilizing computational methods, identified 58 cell types within 6 classes: photoreceptor, horizontal, bipolar, amacrine, ganglion non-neuronal cells. Nearly all are shared between two regions, but there notable differences...
Increased intraocular pressure (IOP) represents a major risk factor for glaucoma, prevalent eye disease characterized by death of retinal ganglion cells; lowering IOP is the only proven treatment strategy to delay progression. The main determinant equilibrium between production and drainage aqueous humor, with compromised generally viewed as primary contributor dangerous elevations. Drainage occurs through two pathways in anterior segment called conventional uveoscleral. To gain insights...
The anterior segment of the eye consists cornea, iris, ciliary body, crystalline lens, and aqueous humor outflow pathways. Together, these tissues are essential for proper functioning eye. Disorders vision have been ascribed to defects in all them; some disorders, including glaucoma cataract, among most prevalent causes blindness world. To characterize cell types that compose tissues, we generated an atlas human using high-throughput single-nucleus RNA sequencing (snRNAseq). We profiled...
Primary open-angle glaucoma (POAG), characterized by retinal ganglion cell death, is a leading cause of irreversible blindness worldwide. However, its molecular and cellular causes are not well understood. Elevated intraocular pressure (IOP) major risk factor, but many patients have normal IOP. Colocalization Mendelian randomization analysis >240 POAG IOP genome-wide association study (GWAS) loci overlapping expression splicing quantitative trait (e/sQTLs) in 49 GTEx tissues retina...
Although the visual system extends through brain, most vision loss originates from defects in eye. Its central element is neural retina, which senses light, processes signals, and transmits them to rest of brain optic nerve (ON). Surrounding retina are numerous other structures, conventionally divided into anterior posterior segments. Here, we used high-throughput single-nucleus RNA sequencing (snRNA-seq) classify characterize cells six extraretinal components segment: ON, head (ONH),...
Proliferative diabetic retinopathy (PDR) is a common cause of blindness in the developed world’s working adult population and affects those with type 1 2 diabetes. We identified Runt-related transcription factor (RUNX1) as gene upregulated CD31+ vascular endothelial cells obtained from human PDR fibrovascular membranes (FVMs) via transcriptomic analysis. In vitro studies using retinal microvascular (HRMECs) showed increased RUNX1 RNA protein expression response to high glucose, whereas...
Glaucoma is a leading cause of blindness. Current glaucoma medications work by lowering intraocular pressure (IOP), risk factor for glaucoma, but most treatments do not directly target the pathological changes to increased IOP, which can manifest as medication resistance disease progresses. To identify physiological modulators we performed genome- and exome-wide association analysis in >129,000 individuals with IOP measurements extended these findings an risk. We report identification...
Although the visual system extends through brain, most vision loss originates from defects in eye. Its central element is neural retina, which senses light, processes signals, and transmits them to rest of brain optic nerve (ON). Surrounding retina are numerous other structures, conventionally divided into anterior posterior segments. Here we used high-throughput single nucleus RNA sequencing (snRNA-seq) classify characterize cells extraretinal components segment: ON, head (ONH), peripheral...
Abstract Primary open-angle glaucoma (POAG), characterized by retinal ganglion cell death, is a leading cause of irreversible blindness worldwide; however, the molecular and cellular causes are not well understood. Elevated intraocular pressure (IOP) major risk factor, but many patients have normal IOP. Colocalization Mendelian randomization analysis >240 POAG IOP GWAS loci overlapping eQTLs sQTLs in 49 GTEx tissues retina prioritized causal genes for 60% loci. These were enriched...
ABSTRACT Most irreversible blindness results from retinal disease. To advance our understanding of the etiology blinding diseases, we used single-cell RNA-sequencing (scRNA-seq) to analyze transcriptomes ∼85,000 cells fovea and peripheral retina seven adult human donors. Utilizing computational methods, identified 58 cell types within 6 classes: photoreceptor, horizontal, bipolar, amacrine, ganglion non-neuronal cells. Nearly all are shared between two regions, but there notable differences...
ABSTRACT The anterior segment of the eye consists cornea, iris, ciliary body, crystalline lens and aqueous humor outflow pathways. Together, these tissues are essential for proper functioning eye. Disorders vision have been ascribed to defects in all them; some, including glaucoma cataract, among most prevalent causes blindness world. To characterize cell types that comprise tissues, we generated an atlas human using high throughput single-nucleus RNA sequencing (snRNAseq). We profiled...
A young man presented with a 1-day history of vision loss in the left eye, eye redness for 1 week, mild posterior headache, and tingling numbness thumb index middle fingers. What would you do next?
HPV (human papillomavirus) is a highly contagious virus that spread by skin-to-skin contact. It can infect the skin, mouth, rectum and anus. also both male female genital areas, including skin on penis or vulva (area around vagina), as well
Purpose: This study examines treatment-based outcomes of endophthalmitis due to antivascular endothelial growth factor (anti-VEGF) intravitreal injection and its effect on subsequent management neovascular disease. Methods: A retrospective multicenter was conducted 157 patients with a diagnosis following anti-VEGF at 10 major ophthalmic centers. Results: The median number injections before (range, 1 84 injections). Initial treatment tap inject or without vitrectomy trended toward smaller...