A5090385359- Glaucoma and retinal disorders
- Endoplasmic Reticulum Stress and Disease
- Nerve injury and regeneration
- Retinal Development and Disorders
- Retinal Diseases and Treatments
- Autophagy in Disease and Therapy
- Electromagnetic wave absorption materials
- Magnetic Properties and Synthesis of Ferrites
- Cell death mechanisms and regulation
- Mitochondrial Function and Pathology
- CRISPR and Genetic Engineering
- Cellular transport and secretion
- Hereditary Neurological Disorders
- Pigment Synthesis and Properties
- Axon Guidance and Neuronal Signaling
- Fire Detection and Safety Systems
- Alzheimer's disease research and treatments
- Pluripotent Stem Cells Research
- Drilling and Well Engineering
- Neurogenetic and Muscular Disorders Research
- Respiratory Support and Mechanisms
- RNA Interference and Gene Delivery
- Mast cells and histamine
- Neuroscience and Neural Engineering
- Hippo pathway signaling and YAP/TAZ
Stanford University
2021-2025
University of Minnesota
2023-2024
Hunan Provincial Maternal and Child Health Hospital
2024
Vision Eye Institute
2024
Chinese University of Hong Kong
2023
Shantou University
2023
Centre for Addiction and Mental Health
2017
Qingdao University
2017
Affiliated Hospital of Qingdao University
2017
Shandong University
2014-2016
The lack of neuroprotective treatments for retinal ganglion cells (RGCs) and optic nerve (ON) is a central challenge glaucoma management. Emerging evidence suggests that redox factor NAD+ decline hallmark aging neurodegenerative diseases. Supplementation with precursors overexpression NMNAT1, the key enzyme in biosynthetic process, have significant effects. We first profile translatomes RGCs naive mice silicone oil-induced ocular hypertension (SOHU)/glaucoma by RiboTag mRNA sequencing....
Optic neuropathy is a group of optic nerve (ON) diseases with progressive degeneration ON and retinal ganglion cells (RGCs). The lack neuroprotective treatments central challenge for this leading cause irreversible blindness. SARM1 (sterile α TIR motif-containing protein 1) has intrinsic nicotinamide adenine dinucleotide (NAD+) hydrolase activity that causes axon by degrading axonal NAD+ significantly after activation injury. deletion in many, but not all, neurodegenerative disease models....
Endoplasmic reticulum (ER) stress has been linked with various acute and chronic neurodegenerative diseases. We previously found that optic nerve (ON) injury diseases induce neuronal ER in retinal ganglion cells (RGCs). further demonstrated germline deletion of CHOP preserves the structure function both RGC somata axons mouse glaucoma models. Here we report RGC-specific and/or its upstream regulator ATF4 synergistically promotes ON survival visual crush silicone oil-induced ocular...
Optineurin (OPTN) mutations are linked to amyotrophic lateral sclerosis (ALS) and normal tension glaucoma (NTG), but a relevant animal model is lacking, the molecular mechanisms underlying neurodegeneration unknown. We find that OPTN C-terminus truncation (OPTN∆C) causes late-onset of retinal ganglion cells (RGCs), optic nerve (ON), spinal cord motor neurons, preceded by decrease axonal mitochondria in mice. discover directly interacts with both microtubules mitochondrial transport complex...
Abstract Recently, we established silicone oil-induced ocular hypertension (SOHU) mouse model with significant glaucomatous neurodegeneration. Here characterize two additional variations of this that simulate distinct glaucoma types. The first is a chronic produced by high frequency (HF) pupillary dilation after SO-induced block, which shows sustained moderate IOP elevation and corresponding slow, mild We also demonstrate although SO removal quickly returns to normal, the neurodegeneration...
Endoplasmic reticulum-associated degradation (ERAD) is a principal quality-control mechanism responsible for targeting misfolded ER proteins cytosolic degradation. Evidence suggests that impairment of ERAD contributes to neuron dysfunction and death in neurodegenerative diseases, many which are characterized by accumulation aggregation proteins. However, the physiological role neurons remains unclear. The Sel1L-Hrd1 complex consisting E3 ubiquitin ligase Hrd1 its adaptor protein Sel1L...
When the protein or calcium homeostasis of endoplasmic reticulum (ER) is adversely altered, cells experience ER stress that leads to various diseases including neurodegeneration. Genetic deletion an downstream effector, CHOP, significantly protects neuron somata and axons. Here we report three tricyclic compounds identified through a small-scale high throughput screening using CHOP promoter-driven luciferase cell-based assay, effectively inhibit by antagonizing their common target, histamine...
Using whole-exome sequencing, we surveyed all the potential pathogenic variants in an SPG42 family and found five SNPs four indels that are shared by two patients lie mapped region. Two variants, SLC33A1 p.Ser113Arg VEPH1 p.Gln433His, cosegregated with disease. However, p.Gln433His was predicted to be tolerated, thus leaving as most plausible causal variant this family. We phosphorylated SMAD1/5/8 (P-SMAD1/5/8) BMP receptor type 1A (BMPR1A) were substantially upregulated fibroblasts derived...
The S113R mutation (c.339T>G) (MIM #603690.0001) in SLC33A1 #603690), an ER membrane acetyl-CoA transporter, has been previously identified patients with hereditary spastic paraplegia type 42 (SPG42; MIM #612539). also shown to inhibit bone morphogenetic protein (BMP) signaling pathway zebrafish. To better understand the function of SLC33A1, we generated and characterized Slc33a1S113R knock-in mice. Homozygous mutant mice were embryonic lethal, while heterozygous Slc33a1...
The unfolded protein response (UPR), which comprises three branches: PERK, ATF6α, and IRE1, is a major mechanism for maintaining cellular proteostasis. Many studies show that the UPR player in regulating neuron viability function various neurodegenerative diseases; however, its role neurodegeneration highly controversial. Moreover, while evidence suggests activation of neurons under normal conditions, deficiency individual branches has no effect on brain animals. It remains unclear whether...
Retinal ganglion cells (RGCs) are heterogeneous projection neurons that convey distinct visual features from the retina to brain. Here, we present a high-throughput in vivo RGC activity assay response light stimulation using noninvasive Ca 2+ imaging of thousands RGCs simultaneously living mice. Population and single-cell analyses longitudinal reveal functional responses unprecedented individual conversions during traumatic glaucomatous degeneration. This study establishes foundation for...
Abstract Optineurin (OPTN) mutations are linked to amyotrophic lateral sclerosis (ALS) and normal tension glaucoma (NTG), but a relevant animal model is lacking, the molecular mechanisms underlying neurodegeneration unknown. We found that OPTN C-terminus truncation (OPTNΔC) causes late-onset of retinal ganglion cells (RGCs), optic nerve (ON), spinal cord motor neurons, preceded by striking decrease axonal mitochondria. Surprisingly, we discover directly interacts with both microtubules...
Abstract Primary open‐angle glaucoma (POAG) is the most common type of glaucoma. Using whole‐exome sequencing, we identified two independent families diagnosed as POAG from China with a novel EFEMP1 variant (Exon3, c.175A>C p.Met59Leu); Three previously reported variants c.1160G>A p.R387Q, c.1189T>C p.Y397H, and c.1429C>T p.R477C in EFEPM1 55 sporadic individuals were also identified. The p.Met59Leu co‐segregated disease phenotype within families. Immunoprecipitation western blot...
Mechanical ventilation is extensively used to treat patients with lung injury but may result in ventilator-induced (VILI). The present study investigated the protective effect of alpha 1-antitrypsin (AAT) on VILI. Adult male rats were subjected sham, + saline, or AAT treatment and injuries evaluated. Peripheral blood bronchoalveolar lavage fluid (BALF) obtained assess systemic local inflammatory responses, respectively. resulted injury, as evidenced by histological abnormalities well...
Cullin 4B (CUL4B) mutations have been implicated in mental retardation and dopamine‐related behaviors due to disruptions their interaction with cullin‐RING E3 ligases (CRLs). Thus, further identification of CUL4B substrates can increase the knowledge protein homeostasis illuminate role neuropsychiatric disease. However, transient nature coupling between its is difficult detect vivo using current approaches, thus hampers efforts investigate functions CRLs within unperturbed living systems. In...
Method of cathode protection is employed to slow down the corrosion terrestrial pipe.This paper aims develop a system monitor pipe equipment wholly track operation status buried timely.The monitoring integrates technology wireless sensor networks, GPRS network and Internet not only collect potential data in time realize remote transmission but also regulate guard mode on demand.Optimization mechanisms are adopted effectively lower energy assumption ensure reliability, from architecture...
Optic neuritis, inflammation, and demyelination of the optic nerve (ON), is one most common clinical manifestations multiple sclerosis; affected patients suffer persistent visual symptoms due to ON degeneration secondary retinal ganglion cell (RGC) death. The mouse experimental autoimmune encephalomyelitis (EAE) model replicates neuritis significant RGC soma axon loss. Nicotinamide mononucleotide adenylyltransferases (NMNATs) are NAD + -synthetic enzymes that have been shown be essential for...
Oil-based drilling fluid is used more and in the field of oil gas exploration. However, because unrecyclable treating agent hard treatment conditions, traditional technologies waste oil-based have some defects, such as resource, bulky equipment, complex processes, low recovery rate. In this work, switchable deoiling (SDA), a novel surfactant for fluid, was synthesized by amine, formic acid, formaldehyde solution. With agent, can be treated without process expensive equipment. Furthermore,...
Abstract CRISPR-Cas nucleases and their nuclease-deactivated dCas variants have revolutionized the field of genome editing gene regulation. Cas12a possesses intrinsic RNAse activity can process multiple functional crRNAs from a single long transcript, making it powerful tool for multiplex targeting. We engineered dCas12a variant termed hyperCas12a with superior efficacy in regulation, especially at restrictive crRNA concentrations. Here, we describe step-by-step protocol constructing...