A5056285519- CRISPR and Genetic Engineering
- Insect and Pesticide Research
- Advanced Graph Neural Networks
- Topic Modeling
- Amphibian and Reptile Biology
- Environmental Toxicology and Ecotoxicology
- Physiological and biochemical adaptations
- Advancements in Battery Materials
- RNA regulation and disease
- SARS-CoV-2 and COVID-19 Research
- Electrocatalysts for Energy Conversion
- RNA and protein synthesis mechanisms
- Hydraulic Fracturing and Reservoir Analysis
- Aluminum Alloys Composites Properties
- Advanced Battery Materials and Technologies
- Advanced materials and composites
- Data Quality and Management
- Species Distribution and Climate Change
- Rock Mechanics and Modeling
- Seismic Imaging and Inversion Techniques
- Algal biology and biofuel production
- Animal Virus Infections Studies
- Advanced Battery Technologies Research
- Animal Behavior and Reproduction
- Biofuel production and bioconversion
China Agricultural University
2018-2025
Institute of Plant Protection
2024-2025
Southwest University
2023-2025
Hong Kong Polytechnic University
2024-2025
Chinese Academy of Agricultural Sciences
2024-2025
Harbin University of Science and Technology
2024-2025
Xiamen University
2020-2024
China University of Petroleum, Beijing
2024
Chinese Academy of Sciences
2020-2024
Shanghai Institutes for Biological Sciences
2021-2024
The type V-F CRISPR-Cas12f system is a strong candidate for therapeutic applications due to the compact size of Cas12f proteins. In this work, we identify six uncharacterized Cas12f1 proteins with nuclease activity in mammalian cells from assembled bacterial genomes. Among them, OsCas12f1 (433 aa) Oscillibacter sp. and RhCas12f1 (415 Ruminiclostridium herbifermentans, which respectively target 5' T-rich Protospacer Adjacent Motifs (PAMs) C-rich PAMs, show highest editing activity. Through...
A SARS-CoV-2 spike protein subunit vaccine induced humoral and cellular immune responses that protected hamsters against infection prevented transmission.
Knowledge graph, or knowledge base, plays an important role in a variety of applications the field artificial intelligence. In both research and application representation learning is one fundamental tasks. Existing approaches are mainly based on structural between entities relations, while among per se largely ignored. Though few integrated entity representations, these methods lack flexibility to apply multimodalities. To tackle this problem, paper, we propose new method, TransAE, by...
With global warming, the contradiction between supply and demand of water resources is increasingly prominent in arid areas. Enhancing resource management an important way to alleviate shortage ecological improve ecosystem services river basins. However, previous studies have rarely conducted comprehensive function zoning using two aspects: category (quantity) strength change (quality), based on changes services. In this regard, scientific problems need be solved:(1) quantifying temporal...
Mutations in the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike receptor-binding domain (RBD) may alter viral host tropism and affect activities of neutralizing antibodies. Here, we investigated 153 RBD mutants 11 globally circulating variants concern (VOCs) interest (VOIs) (including Omicron) for their antigenic changes cross-species cells expressing 18 ACE2 orthologs. Several mutations strengthened infectivity orthologs non-human animals, particularly those less...
High-capacity rechargeable batteries are crucial for portable electronics, electric vehicles, and smart power grids. High-quality porous Al current collectors with well-designed hole size density expected to strengthen lithium transportation as well accommodate volume variations during fast charging discharging. Ultrafast femtosecond laser drilling is exploited in this paper fabricate 3D precisely controlled porosity. With optimized processing parameters, a series of high-quality foils...
In this work, a series of CoCrFeNiCeX (x = 0.1, 0.3, 0.5) high-entropy alloys (HEAs) were designed and prepared through vacuum induction melting. The microstructure, wear resistance antibacterial behaviors the HEAs systematically investigated. results showed that are composed FCC phase with dendrite structure CeNi3 between dendrites. With increase Ce content, mechanical property rate was improved gradually. Due to combined effect fine grain strengthening, CoCrFeNiCe0.5 HEA exhibited high...
In order to promote the green development of agriculture, it is important study enantioselective effect chiral pesticides. The bioactivity fungicide penthiopyrad (PEN) racemate and enantiomers against phytopathogens, toxicity non-target organisms, on tomato fruit growth maturation, environmental fate in cultivation were evaluated at an level this study.The results indicated that same efficacy, optically pure S-(+)-PEN could lower dosage by 20-96%. S-enantiomer had low earthworms. Besides,...
Abstract Catalytically inactive CRISPR‐Cas13 (dCas13)‐based base editors can achieve the conversion of adenine‐to‐inosine (A‐to‐I) or cytidine‐to‐uridine (C‐to‐U) at RNA level, however, large size dCas13 protein limits its in vivo applications. Here, a compact and efficient editor (ceRBE) is reported with high editing efficiency. The larger replaced 199‐amino acid EcCas6e protein, derived from Class 1 CRISPR family involved pre‐crRNA processing, conducted optimization for toxicity ceRBE...
Abstract Recognition of specific chromatin modifications by distinct structural domains within “reader” proteins plays a critical role in the maintenance genomic stability. However, mechanisms involved this process remain unclear. Here we report that PHD-Bromo tandem domain tripartite motif-containing 66 (TRIM66) recognizes unmodified H3R2-H3K4 and acetylated H3K56. The aberrant deletion Trim66 results severe DNA damage instability embryonic stem cells (ESCs). Moreover, find recognition...