A5101481989- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- RNA Interference and Gene Delivery
- RNA regulation and disease
- Hearing, Cochlea, Tinnitus, Genetics
- Vestibular and auditory disorders
- Neuroscience and Neuropharmacology Research
- Retinal Development and Disorders
- Alzheimer's disease research and treatments
- Effects of Radiation Exposure
- Cancer-related molecular mechanisms research
- Neuroinflammation and Neurodegeneration Mechanisms
- DNA Repair Mechanisms
- Virus-based gene therapy research
- RNA and protein synthesis mechanisms
- Congenital heart defects research
- Extracellular vesicles in disease
- Neurological diseases and metabolism
- Renal and related cancers
- Genomics and Chromatin Dynamics
- Angiogenesis and VEGF in Cancer
- SARS-CoV-2 and COVID-19 Research
- Nuclear Receptors and Signaling
- Viral Infections and Immunology Research
- Genetics, Aging, and Longevity in Model Organisms
Center for Excellence in Brain Science and Intelligence Technology
2017-2025
Chinese Academy of Sciences
2017-2025
Shanghai Center for Brain Science and Brain-Inspired Technology
2021-2025
Shanghai Institutes for Biological Sciences
2017-2024
University of Chinese Academy of Sciences
2021
Neuroscience Institute
2018
Yichang Central People's Hospital
2018
China Three Gorges University
2018
Erasmus University Rotterdam
2014-2016
Erasmus MC
2014-2016
Due to the uniform cyto-architecture of cerebellar cortex, its overall physiological characteristics have traditionally been considered be homogeneous. In this study, we show in awake mice at rest that spiking activity Purkinje cells, sole output cells differs between modules and correlates with their expression glycolytic enzyme aldolase C or zebrin. Simple spike complex frequencies were significantly higher located zebrin-negative than zebrin-positive modules. The difference simple...
Targeted integration of transgenes can be achieved by strategies based on homologous recombination (HR), microhomology-mediated end joining (MMEJ) or non-homologous (NHEJ). The more generally used HR is inefficient for achieving gene in animal embryos and tissues, because it occurs only during cell division, although MMEJ NHEJ elevate the efficiency some systems. Here we devise a homology-mediated (HMEJ)-based strategy, using CRISPR/Cas9-mediated cleavage both transgene donor vector that...
Abstract Loss-of-function mutations in the gene encoding postsynaptic scaffolding protein SHANK2 are a highly penetrant cause of autism spectrum disorders (ASD) involving cerebellum-related motor problems. Recent studies have implicated cerebellar pathology aetiology ASD. Here we evaluate possibility that Purkinje cells (PCs) represent critical locus ASD-like pathophysiology mice lacking Shank2 . Absence impairs both PC intrinsic plasticity and induction long-term potentiation at parallel...
Engineered extracellular vesicles (EVs) are considered excellent delivery vehicles for a variety of therapeutic agents, including nucleic acids, proteins, drugs, and nanomaterials. Recently, several studies have indicated that clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9) delivered by EVs enable efficient DNA editing. However, an RNA editing tool is still unavailable. Here, signal peptide-optimized EVs-delivered guide (gRNA) CRISPR/CasRx...
RNA-targeting CRISPR system Cas13 offers an efficient approach for manipulating RNA transcripts in vitro. In this perspective, we provide a proof-of-concept demonstration that Cas13-mediated Vegfa knockdown vivo could prevent the development of laser-induced CNV mouse model Age-related macular degeneration.
The massive computational capacity of the cerebellar cortex is conveyed by Purkinje cells onto and vestibular nuclei neurons through their GABAergic, inhibitory output. This implies that pauses in cell simple spike activity are potentially instrumental information processing, but occurrence extent still heavily debated. cortex, although often treated as such, not homogeneous. Cerebellar modules with distinct anatomical connectivity gene expression have been described, these also differ...
To supply tumor tissues with nutrients and oxygen, endothelial progenitor cells (EPCs) home to sites contribute neovascularization. Although the precise mechanism of EPCs‐induced neovascularization remains poorly understood in non‐small cell lung cancer (NSCLC), histone deacetylase 7 (HDAC7) is considered as a critical regulator. explore function HDAC7 induced by EPCs, tube formation assay, immunofluorescence, microarray, Western blot analysis animal models were performed. In vitro ,...
The response to low doses of X rays was assessed in cells three hamster cell lines which are defective DNA repair and compared with their parental lines. Cells the V79-derived double-strand break repair-deficient line XR-V15B showed no radioresistance 0.5-Gy range V79B wild type, but instead an exponential response. single-strand EM9 hyper-radiosensitivity exhibited increased radioresistance. Most interestingly, UV-20 appeared respond exponentially, as a continuation hyper-radiosensitive...
The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has resulted in catastrophic damage worldwide. Accordingly, the development of powerful, safe, easily accessible vaccines with long-term effectiveness is understood as an urgently needed countermeasure against this ongoing pandemic. Guided strong promise using AAVs, we here designed, optimized, and developed AAV-based (including AAV-RBD(max), AAV-RBD(wt), AAV-2xRBD,...
A preliminary investigation of the effect topoisomerase inhibitors on structure survival curve at low doses has been carried out in Chinese hamster V79 cells, where there is a deviation from predicted response to radiation (Marples and Joiner, Radiat. Res. 113, 41-51, 1993). Cells were treated with one representative drug for each enzyme (topoisomerase I or II) prior X irradiation air assessed cell using automated microscopic location cells. VP-16 causes little no effect, while camptothecin...
SUMMARY Interspecies organogenesis via blastocyst complementation provides a unique platform to study development in an evolutionarily context and holds potential overcome world-wide organ shortages 1 . By using this technique, rat pancreas, thymus, heart, eye tissues have been generated mice 2–4 To date, however, xeno-generation of brain has not achieved through complementation. Here, we developed optimized one-step strategy based on C-CRISPR 5 , which facilitated rapid screening candidate...
Abstract Williams syndrome is a developmental disorder caused by microdeletion entailing loss of single copy 25-27 genes on chromosome 7q11.23. Patients with suffer from cardiovascular and neuropsychological symptoms. So far, the structural abnormalities system in have been attributed to elastin ( ELN ) gene. In contrast, consequences syndrome, including motor deficits, hypersociability cognitive impairments, mainly altered expression transcription factors like LIMK1, GTF2I GTF2IRD1, while...
DNA variants in or closed to the human TBX15 and PAX1 genes have been repeatedly associated with facial morphology independent genome-wide association studies, while their functional roles determining remains be understood. We generated Tbx15 knockout ( -/- ) Pax1 mice by applying one-step CRISPR/Cas9 method. A total of 75 adult were used for subsequent phenotype analysis, including 38 (10 homozygous , 18 heterozygous +/- 10 wild-type WT) 37 (12 15 WT mice). Facial other physical...
The human face represents a combined set of highly heritable phenotypes, but knowledge on its genetic architecture remains limited. A series genome-wide association studies 78 facial shape phenotypes quantified from 3-dimensional images 10,115 Europeans identified 24 loci, among which 17 were previously unpublished. multi-ethnic replication study in 7,917 individuals confirmed 13 loci including 8 unpublished ones. Allele frequencies at the face-associated significantly more differentiated...
Abstract The smallest Cas13 family protein, CasRx, has a high cleavage activity and targeting specificity, offering attractive opportunity for therapeutic applications. Here we report that delivery of CasRx by adeno-associated virus via intravitreal injection could efficiently knockdown Vegfa transcripts significantly reduce the area laser-induced choroidal neovascularization in mouse model age-related macular degeneration. Thus, RNA-targeting CRISPR system be used vivo gene therapy.
Abstract Detection of genetic information in the natural context is essential to understand biological principles. However, ability sense activity endogenous genes limited for conventional tools. Here, we developed a highly programmable sgRNA switch system (Ents) that enables detection and lncRNAs, even with very low expression level. This protocol related publication “Endogenous promoter-driven monitoring low-abundant lncRNAs” Nature Cell Biology.