A5083326009- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Birth, Development, and Health
- RNA modifications and cancer
- Pregnancy and preeclampsia studies
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Wnt/β-catenin signaling in development and cancer
- RNA Research and Splicing
- Epigenetics and DNA Methylation
- Tissue Engineering and Regenerative Medicine
- Mesenchymal stem cell research
- Bioinformatics and Genomic Networks
- Fungal and yeast genetics research
- Protein Structure and Dynamics
- Microbial Metabolic Engineering and Bioproduction
- Cytokine Signaling Pathways and Interactions
- Ovarian function and disorders
- Neurogenesis and neuroplasticity mechanisms
- Cancer-related gene regulation
- Extracellular vesicles in disease
- Genetic Associations and Epidemiology
- Biofuel production and bioconversion
- Circular RNAs in diseases
- Apelin-related biomedical research
Second Hospital of Shanxi Medical University
2025
Shanxi Medical University
2025
Fudan University
2012-2024
State Key Laboratory of Genetic Engineering
2016-2024
Massachusetts General Hospital
2004-2022
Harvard University
2022
Academy of Military Transportation
2020
Huashan Hospital
2019
State Key Laboratory of Medical Neurobiology
2019
Obstetrics and Gynecology Hospital of Fudan University
2016
Fibroblasts can be reprogrammed to induced pluripotent stem cells (iPSCs) by application of transcription factors octamer-binding protein 4 (Oct4), SRY-box containing gene 2 (Sox2), Kruppel-like factor (Klf4), and c-Myelocytomatosis oncogene (c-Myc) (OSKM), but the underlying mechanisms remain unclear. Here, we report that exogenous Oct4 Sox2 bind at promoter regions mir-141/200c mir-200a/b/429 cluster, respectively, induce activation miR-200 family during OSKM-induced reprogramming....
Abstract Pulmonary fibrosis (PF) is a severe pulmonary disease with limited available therapeutic choices. Recent evidence increasingly points to abnormal lipid metabolism as critical factor in PF pathogenesis. Our latest research identifies the dysregulation of low-density lipoprotein (LDL) new risk for PF, contributing alveolar epithelial and endothelial cell damage, fibroblast activation. In this study, we first integrative summarize published literature about metabolite changes found...
Clarifying the regulatory mechanisms of embryonic stem cell (ESC) neural differentiation is helpful not only for understanding development but also obtaining high-quality progenitor cells required by therapy neurodegenerative diseases. Here, we found that long noncoding RNA 1604 (lncRNA-1604) was highly expressed in cytoplasm during differentiation, and knockdown lncRNA-1604 significantly repressed mouse ESCs both vitro vivo. Bioinformatics prediction mechanistic analysis revealed functioned...
Elevated homocysteine levels are known to be a risk factor for congenital heart disease (CHD), but the mechanism underlying this effect is unknown. During early embryonic development, removal dictated exclusively by MTR activity. To examine role of in CHD risk, we identified genetic variants and investigated mechanisms that affect its expression increase Chinese populations. The association between regulatory gene was examined three independent case–control studies total 2340 patients with...
Angiogenesis is crucially involved in many physiological and pathological processes including tumor growth, but the molecular mechanisms regulating angiogenesis are incompletely understood. In this study, we investigated functions mechanism of histone deacetylase 10 (HDAC10), a member HDAC II family, regulation angiogenesis. HDAC10 overexpression human umbilical vein endothelial cells (HUVECs) promoted tube formation, whereas depletion from HUVECs inhibited formation vitro vivo....
Background Osteoarthritis (OA) is a prevalent chronic joint disease for which there lack of effective treatments. In this study, we used Mendelian randomization analysis to identify circulating proteins that are causally associated with OA-related traits, providing important insights into potential drug targets OA. Method Causal associations between 1553 and five traits were assessed in large-scale two-sample MR analyses using Wald ratio or inverse variance weighting, the results corrected...
Long noncoding RNAs (lncRNAs) play a wide range of roles in the epigenetic regulation crucial biological processes, but functions lncRNAs cortical development are poorly understood. Using human embryonic stem cell (hESC)-based 2D neural differentiation approach and 3D cerebral organoid system, we identified that lncRNA PAUPAR, which is adjacent to PAX6, plays essential by interacting with PAX6 regulate expression large number genes. Mechanistic studies showed PAUPAR confers proper binding...
Induced pluripotent stem (iPS) cells were first generated by forced expression of transcription factors (TFs) in fibroblasts. Recently, iPS have been more rapidly and efficiently using miRNAs with or without other factors. However, the specific collaborative roles pluripotency acquisition maintenance remain to be further investigated. Here, based on miRNA profiling mouse embryonic fibroblasts (MEFs), MEFs infected Oct3/4, Sox2, Klf4 c-Myc (OSKM) for 1, 2, 4, 8 day, two cell lines ES cells,...
Fetal growth restriction (FGR) contributes the primary cause of perinatal mortality and morbidity with impacts on long-term health. To determine core gene expression network signatures, which in combination ultrasound confirmation will more effectively differentiate constitutionally normal small for gestational age pathological FGR groups, we performed RNA sequencing protein-coding genes, lncRNAs, RNAs a case-control study umbilical cord blood. Five pairs case control blood samples were used...
Abstract DNA methylation and histone (H3K27me3) have been reported as major barriers to induced pluripotent stem cell (iPSC) generation using four core transcription factors (Oct4, Sox2, Klf4, c-Myc, termed OSKM). Here, illustrate the possibility of deriving iPSCs via demethylation, well exact effects modification on gene expression regulation, we performed RNA sequencing characterize transcriptomes ES cells derived by demethylation with miR-29b or shDnmt3a, carried out integrated analyses....
Recently, the debate on centrality-lethality rule is resolved by "second-generation" high-throughput Y2H data from yeast interactome network, which suggests no significant correlation between degree of connectedness and essentiality proteins. However, it still not clear why essential proteins strongly tend to interact with each other. Previously, concept protein-protein interactions was proposed explain mechanism underlying clustering In this article we show that 67 75% excessive (IBEPs) in...
Large-intergenic noncoding RNAs (lincRNAs) cooperate with core transcription factors to coordinate the pluripotency network of embryonic stem cells. The mechanisms by which lincRNAs affect chromatin structure and gene remain mostly unknown. Here, we identified that a lincRNA (linc1614), occupied at its promoter, was indispensable for both maintenance acquisition pluripotency. Linc1614 served as specific partner factor Sox2 in maintaining pluripotency, primarily mediating function repression...
Abstract Embryonic stem (ES) cells have the property of self-renewal and multi-directional differentiation, provide an ideal model for studying early embryo development in vitro. Wnt3, as Wnt family member 3, plays a vital role during ES cell differentiation. However, exact regulatory mechanism Wnt3 remains to be elucidated. MicroRNAs can directly regulate gene expression at post-transcriptional level play critical function fate determination. Here, we found miR-184 decreased when...
Innate lymphoid cells (ILCs) develop from ILC progenitors in the bone marrow. Various precursors (ILCPs) with different subset lineage potentials have been identified based on expression of cell surface markers and ILC-associated key transcription factor reporter genes. This study characterized an interleukin (IL)-7Rα + IL-18Rα progenitor population mouse marrow multi-ILC potential clonal level. Single-cell gene analysis revealed heterogeneity this several subpopulations specific...
Abstract Phages, the natural predators of bacteria, were discovered more than 100 years ago. However, increasing antimicrobial resistance rates have revitalized phage research. Methods that are time-consuming and efficient wet-laboratory experiments needed to help screen phages quickly for therapeutic use. Traditional computational methods usually ignore fact phage-bacteria interactions achieved by key genes proteins. intraspecific prediction rare since almost all existing consider only at...
Embryonic stem cells (ESCs) have self-renewal and multi-lineage differentiation potential perform critical functions in development biomedicine. Several long noncoding RNAs (lncRNAs) been reported as key regulators of cell pluripotency differentiation. However, the function regulatory mechanism lncRNAs during initiation ESC remains unclear. Here, we found that linc1557 was highly expressed mouse ESCs required for Knockdown increased expression phosphorylation levels signal transducer...