A5065552266- DNA Repair Mechanisms
- Epigenetics and DNA Methylation
- Retinal Development and Disorders
- Microtubule and mitosis dynamics
- Aldose Reductase and Taurine
- Corneal Surgery and Treatments
- Axon Guidance and Neuronal Signaling
- Ubiquitin and proteasome pathways
- Neurogenesis and neuroplasticity mechanisms
- Prenatal Substance Exposure Effects
- Fibroblast Growth Factor Research
- Connexins and lens biology
- Adenosine and Purinergic Signaling
- Genomics and Chromatin Dynamics
- Connective Tissue Growth Factor Research
- Developmental Biology and Gene Regulation
- Nerve injury and regeneration
- Neurological Disorders and Treatments
- Cellular Mechanics and Interactions
- Retinal Diseases and Treatments
- Cancer Genomics and Diagnostics
- Neuroinflammation and Neurodegeneration Mechanisms
- S100 Proteins and Annexins
University of Pittsburgh
2021-2024
Center for Cancer Research
2016-2021
National Cancer Institute
2016-2021
University of Maryland, College Park
2017
National Institutes of Health
2016
As part of the central nervous system (CNS), retinal ganglion cells (RGCs) and their axons are only neurons in retina that transmit visual signals from eye to brain via optic nerve (ON). Unfortunately, they do not regenerate upon injury mammals. In ON trauma, microglia (RMG) become activated, inducing inflammatory responses resulting axon degeneration RGC loss. Since aldose reductase (AR) is an response mediator highly expressed RMG, we investigated if pharmacological inhibition AR can...
Abstract Safeguards against excess DNA replication are often dysregulated in cancer, and driving cancer cells towards over-replication is a promising therapeutic strategy. We determined synthesis patterns undergoing partial genome re-replication due to perturbed regulatory interactions (re-replicating cells). These exhibited slow replication, increased frequency of initiation events, skewed pattern that preferentially reactivated early-replicating origins. Unlike exposed stress, which...
Abstract Mammalian chromosome replication starts from distinct sites; however, the principles governing initiation site selection are unclear because proteins essential for DNA do not exhibit sequence-specific binding. Here we identify a replication-initiation determinant (RepID) protein that binds subset of sites. A large fraction RepID-binding sites share common G-rich motif and elevated initiation. RepID is required RepID-bound origins, including origin at human beta-globin (HBB) locus....
Mammalian central nervous system (CNS) axons cannot spontaneously regenerate after injury, creating an unmet need to identify molecular regulators promote axon regeneration and reduce the lasting impact of CNS injuries. While tubulin polymerization promoting protein family member 3 (Tppp3) is known outgrowth in amphibians, its role mammalian remains unknown. Here we investigated Tppp3 retinal ganglion cells (RGCs) neuroprotection axonal using optic nerve crush (ONC) model rodent. Single-cell...
We sought to evaluate the efficacy of growth differentiation factor (GDF)-15 treatment for suppressing epithelial-mesenchymal transition (EMT) and alleviating transforming β2 (TGFβ2)-induced lens opacity.
Abstract We determined replication patterns in cancer cells which the controls that normally prevent excess were disrupted (“re-replicating cells”). Single-fiber analyses suggested origins activated at a higher frequency re-replicating cells. However, nascent strand sequencing demonstrated utilized same pool of potential as replicating Surprisingly, exhibited skewed initiation correlating with timing. These differed from profiles observed non-re-replicating exposed to stress, novel group...