A5077740388- Retinal Development and Disorders
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Ocular Oncology and Treatments
- Neuroscience and Neural Engineering
- Retinal Diseases and Treatments
- Cancer-related Molecular Pathways
- Photoreceptor and optogenetics research
- Corneal Surgery and Treatments
- Glaucoma and retinal disorders
- Retinopathy of Prematurity Studies
- Fungal Infections and Studies
- Antifungal resistance and susceptibility
- Multiple Sclerosis Research Studies
- Retinal and Macular Surgery
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- Extracellular vesicles in disease
- Photochromic and Fluorescence Chemistry
- Tissue Engineering and Regenerative Medicine
- 3D Printing in Biomedical Research
- RNA Research and Splicing
- Nail Diseases and Treatments
- Systemic Lupus Erythematosus Research
- RNA and protein synthesis mechanisms
China Academy of Space Technology
2025
Sun Yat-sen University
2015-2024
Key Laboratory of Guangdong Province
2022
Zhongshan Ophthalmic Center, Sun Yat-sen University
2005-2020
Johns Hopkins Medicine
2010-2018
Johns Hopkins University
2010-2018
StemCells (United States)
2018
The People's Hospital of Guangxi Zhuang Autonomous Region
2007
Zero to Three
2007
Third Affiliated Hospital of Sun Yat-sen University
2007
Purpose.: We sought to determine if human induced pluripotent stem cells (iPSCs) derived from blood could produce optic vesicle–like structures (OVs) with the capacity stratify and express markers of intercellular communication. Methods.: Activated T-lymphocytes a routine peripheral sample were reprogrammed by retroviral transduction iPSCs. The T-lymphocyte–derived iPSCs (TiPSCs) characterized for pluripotency differentiated OVs using our previously published protocol. TiPSC-OVs then...
Blood-retinal barrier [BRB] breakdown, characteristic of diabetic retinopathy (DR), is believed to depend on inflammation and apoptosis. Retinal almost completely suppressed in the absence TNFα, which also associated with This study was conducted determine role TNFα these complications.Diabetes induced streptozotocin Tnfa knockout (KO) mice, provide a chemical model diabetes, mice were crossed Ins2(Akita) generate genetic model, both models being devoid TNFα. The BRB assessed at 1, 1.5, 3, 6...
Purpose.: Somatic cells can be reprogrammed into an embryonic stem cell–like pluripotent state by Oct-3/4, Sox2, c-Myc, and Klf4. Sox2 as essential reprogramming factor also contributes to the development of eye retina. This study was conducted determine whether induced (iPS) express retinal progenitor cell (RPC)-related genes iPS directly differentiate ganglion (RGCs). Methods.: Mouse were ectopically expressed four factors in tail-tip fibroblasts (TTFs). The expression RPC-related analyzed...
The derivation and maintenance of human pluripotent stem cells (hPSCs) in stable naïve states has a wide impact developmental biology. However, hPSCs are unstable classical mouse embryonic cell (ESC) WNT MEK/ERK signal inhibition (2i) culture. We show that broad repertoire conventional hESC transgene-independent induced (hiPSC) lines could be reverted to preimplantation inner mass (ICM)-like with only WNT, MEK/ERK, tankyrase (LIF-3i). LIF-3i-reverted retained normal karyotypes genomic...
Background: Oxidative stress plays a central role in neuropathology of multiple sclerosis (MS). The patients with MS have low antioxidant status. Antioxidant therapy may represent an attractive treatment MS. However, the relationship between neuromyelitis optica (NMO), distinct nosologic entity or form MS, and status has not fully been investigated. Objectives: To investigate correlation NMO serum uric acid (UA), bilirubin albumin. Methods: levels molecules UA, (Tbil Ibil) albumin were...
The mechanisms underlying retinal development have not been completely elucidated. Extracellular vesicles (EVs) are novel essential mediators of cell-to-cell communication with emerging roles in developmental processes. Nevertheless, the identification EVs human tissue, characterization their cargo, and analysis potential role retina has accomplished. Three-dimensional tissue derived from induced pluripotent stem cells (hiPSC) provide an ideal system to achieve this goal. Here we report that...
Aiming at the development requirements of miniaturization and high performance motor controller, a miniaturized high-speed permanent magnet synchronous (PMSM) controller without position sensors based on FPGA is designed in this paper. The uses three-phase full-bridge inverter circuit to drive control PMSM achieve rated speed 60000r/min. replaces realize commutation detection by means back electromotive force(back-EMF) zero-crossing detection, realizes winding current sampling reconstructing...
All cancers develop from malignant transformation of normal cells, while it is challenging to identify these cells-of-origin since impossible witness the dynamic changes in human cancers. Retinoblastoma (Rb), an intraocular cancer, a typical model for study molecular and cellular mechanisms Although maturing cone precursors (CPs) are proposed as origin Rb, unknow whether other retinal cells also sensitive RB1 inactivation. Here we developed RB1-deficient organoids (ROs) models RB1-/- or...
Abstract Aiming at the reconstruction problem of Hall drift current during operation thrusters, this study focuses on compensating accuracy reduced by magnetic signal loss. It investigated influence sensor loss reconstruction, and elaborates applying Kriging interpolation to improve accuracy, so as enhance anti-interference ability magnetostatic inversion method in practical applications. This utilizes make up missing signals then uses interpolated field reconstruct current. The obtained...
The serum uric acid (UA) levels were measured in 112 patients with multiple sclerosis (MS) and 794 different types of other neurological diseases (OND) or healthy control group. Serum UA levels, along relevant clinical parameters MS OND, also investigated. had significantly lower than those transient ischemia attack (344.6 ± 130.6 µmol/L, P = 0.000), cerebral hemorrhage (311.9 104.7 infarction (291.3 101.6 0.014) the group (312.1 92.8 0.000). higher cryptococcus meningitis...
Urine cells, a body trash, have been successfully reprogrammed into human induced pluripotent stem cells (U-hiPSCs) which hold huge promise in regenerative medicine. However, it is unknown whether or to what extent U-hiPSCs can generate retinal so far. With modified differentiation protocol without addition of retinoic acid (RA), our study revealed that were able differentiate towards fates and form 3D organoids containing laminated neural retina with all cell types located proper layer as...
Abstract Retinoblastoma (RB) is the most common malignant tumor of retina in human children. Although it has been hypothesized for a long time that RB derives from multipotent retinal stem cells (RSCs) or retinoblasts, direct evidence presence tumorigenic RSCs tumors still lacking. Some studies indicate contain similar to their normal tissue cell counterparts. With vitro culture and differentiation method we demonstrate stem‐like (RSLCs) indeed exist lesions tumor‐derived cultures encompass...
Abstract Effective derivation of three-dimensional (3D) retinal tissue from human-induced pluripotent stem cells (hiPSCs) could provide models for drug screening and facilitate patient-specific cell replacement therapy. However, some hiPSC lines cannot undergo 3D self-organization show inadequate differentiation efficiency to meet clinical demand. In this study, we developed an optimized system tissue. We found that the Wnt signaling pathway antagonist Dickkopf-related protein 1 (DKK-1)...
RPE65-associated Leber congenital amaurosis (LCA) is one of highly heterogeneous, early onset, severe retinal dystrophy with at least 130 gene mutation sites identified. Their pathogenicity has not been directly clarified due to lack diseased cells. Here, we generated human induced pluripotent stem cells (hiPSCs) from putative LCA patient carrying two novel RPE65 mutations c.200T>G (p.L67R) and c.430T>C (p.Y144H), named RPE65-hiPSCs, which were confirmed contain the same mutations. The...
Induced pluripotent stem cells (iPSCs) have provided new opportunities for motor neuron disease (MND) modeling, drug screening, and cellular therapeutic development. Among the various types of iPSCs, urine-derived iPSCs become a promising source because they can be safely noninvasively isolated easily reprogrammed. Here, first time, we differentiated (urine-iPSCs) into neurons (MNs) compared capacity urine-iPSCs cord-blood-derived (B-iPSCs) to differentiate MNs. With use small molecules,...
Purpose: Retinal pigment epithelium (RPE) and neural retina could be generated concurrently through retinal organoid induction approaches using human induced pluripotent stem cells (hiPSCs), providing valuable sources for cell therapy of degenerations. This study aims to enrich expand hiPSC-RPE acquired with this platform explore characteristics serially passaged RPE cells. Methods: has been differentiated from hiPSCs a published method. After detachment on the 4th week, remaining mixture...
This study was conducted to determine the dynamic Islet1 and Brn3 (POU4F) expression pattern in human fetal retina human-induced pluripotent stem cell- (hiPSC-) derived retinal organoid. Human eyes from 8 27 weeks (Fwks), adult retina, hiPSC-derived organoid 7 31 differentiation (Dwks), rhesus were collected for cyrosectioning. Immunofluorescence analysis showed that expressed ganglion cells organoids. Unexpectedly, after Fwk 20, gradually decreased retina. In midstage of development,...
Human pluripotent stem cell-derived retinal pigment epithelium (iRPE) is an attractive cell source for disease modeling and replacement therapy of disorders with RPE defects. However, there are still challenges to develop appropriate culture conditions close in vivo microenvironment generate iRPE sheets, which mimic more faithfully the characteristics functions human cells. Here, we developed a simple, novel platform construct authentic sheets using amniotic membrane (hAM) as natural...
Aim: The aim of this study was to investigate morphologic changes retina and sclera in form-deprived myopic rabbits following intravitreal dopamine injection. Methods: Neonatal were monocularly deprived form vision by suturing the right eyelids after natural eye opening. In deprivation (FD) group, received FD alone. dopamine–form (DA-FD) an injection 20 μg every 5 days for a total 4 injections. saline-FD saline injections same schedule as DA-FD group. untreated contralateral eyes used...