A5081909652- Sarcoma Diagnosis and Treatment
- Lymphoma Diagnosis and Treatment
- CRISPR and Genetic Engineering
- Acute Lymphoblastic Leukemia research
- Hematopoietic Stem Cell Transplantation
- CAR-T cell therapy research
- Pluripotent Stem Cells Research
- Transplantation: Methods and Outcomes
- Mechanical Circulatory Support Devices
- Renal and related cancers
- Childhood Cancer Survivors' Quality of Life
- Simulation-Based Education in Healthcare
- Testicular diseases and treatments
- Polyomavirus and related diseases
- Respiratory Support and Mechanisms
- Neuroscience of respiration and sleep
- Cancer Cells and Metastasis
- Family and Patient Care in Intensive Care Units
- Ultrasound in Clinical Applications
- Cancer Research and Treatments
- Cardiovascular Issues in Pregnancy
- Blood donation and transfusion practices
- Genetic Syndromes and Imprinting
- Acute Myocardial Infarction Research
- Pulmonary Hypertension Research and Treatments
Johns Hopkins University
2015-2024
Johns Hopkins Medicine
2015-2024
Bloomberg (United States)
2021
Children's Center
2021
Sidney Kimmel Comprehensive Cancer Center
2016-2017
University of Baltimore
2016
Johns Hopkins Hospital
2016
The derivation and maintenance of human pluripotent stem cells (hPSCs) in stable naïve states has a wide impact developmental biology. However, hPSCs are unstable classical mouse embryonic cell (ESC) WNT MEK/ERK signal inhibition (2i) culture. We show that broad repertoire conventional hESC transgene-independent induced (hiPSC) lines could be reverted to preimplantation inner mass (ICM)-like with only WNT, MEK/ERK, tankyrase (LIF-3i). LIF-3i-reverted retained normal karyotypes genomic...
Allogeneic hematopoietic stem cell transplantation (HSCT) is curative for many nonmalignant pediatric disorders, including hemoglobinopathies, bone marrow failure syndromes, and immunodeficiencies. There great success using HLA-matched related donors these patients; however, the use of alternative has been associated with increased graft failure, graft-versus-host disease (GVHD), transplant-related mortality (TRM). HSCT post-transplantation cyclophosphamide (PT/Cy) GVHD prophylaxis performed...
Even though virtually all patients with Ewing sarcoma achieve a radiographic complete response, up to 30% of who present localized disease and 90% those metastases experience metastatic recurrence, highlighting the inability identify residual at end therapy. Up 95% sarcomas carry driving EWS-ETS translocation that has an intronic breakpoint is specific each tumor, authors developed system quantitatively detect DNA fragment in patient plasma.The used long-range multiplex polymerase chain...
Rapid-onset obesity with hypothalamic dysfunction, hypoventilation, and autonomic dysregulation (ROHHAD) is a rare, generally progressive, potentially fatal syndrome of unclear etiology. The characterized by normal development followed sudden, rapid hyperphagic weight gain beginning during the preschool period, central often accompanied personality changes developmental regression, leading to substantial morbidity mortality. We describe 2 children who had symptomatic neuropsychological...
Abstract Background Outcomes for patients with oncologic disease and/or after hematopoietic stem cell transplant (HSCT) requiring intensive care unit admission have improved, but indications and outcomes extracorporeal membrane oxygenation (ECMO) support in this population are poorly characterized. Procedure We analyzed data from consecutive < 18 years HSCT reported to a pediatric ECMO registry by nine centers the United States between 2011 2018. Results identified HSCT, 415 controls...
Introduction: Children in resource-limited settings are disproportionately affected by common childhood illnesses, resulting high rates of mortality. A major barrier to improving child health such regions is limited pediatric-specific training, particularly the care children with critical illness. While global rotations for trainees from North America and Europe have become commonplace, residency fellowship programs struggle ensure that these mutually beneficial do not place an undue burden...
Prior research on red blood cell (RBC) storage duration and clinical outcomes in paediatric cardiac surgery has shown conflicting results. The purpose of this study was to evaluate whether stored for a longer is harmful these patients.We performed retrospective cohort patients undergoing at our institution between January 2011 June 2015. Patients were stratified based they transfused RBCs ≤15 days (fresher blood) or >15 (older blood). primary outcome composite morbidity, with prolonged...
Hematopoietic stem cell transplantation (HSCT) is curative for many non-malignant pediatric disorders, including hemoglobinopathies, bone marrow failure syndromes, and immunodeficiencies. There has been great success using HLA-matched related donors; however, use of alternative donors associated with increased graft failure, versus host disease (GVHD), transplant-related mortality (TRM). HSCT post-transplantation cyclophosphamide (PT/Cy) performed hematologic malignancies engraftment, GVHD,...
Abstract The inability to effectively treat hematogenous metastasis remains the largest challenge of sarcoma therapy. ability isolate, quantify, and study circulating tumor cells (CTC) in sarcomas has potential enhance our understanding biology metastasis, altering way we patients with high grade sarcomas. Using a size based filtration device, CellSieve™, were able successfully identify quantify CTC metastatic xenograft model as well blood samples from In mice, utilized orthotopic...
Human pluripotent stem cells (hPSC) share developmental, biochemical, and epigenetic commonalities with "primed" rodent post-implantation epiblast (EpiSC), which possess a less primitive, more restricted lineage-primed functional pluripotency. The derivation of stable hPSC in naïve ground states has wide impact human developmental biology. However, are unstable classical state mouse ESC (mESC) WNT MEK/ERK signal inhibition (2i) culture conditions. Recently described have required complex...