A5103112188- Cancer Cells and Metastasis
- Bacterial Infections and Vaccines
- Cancer Genomics and Diagnostics
- Pneumonia and Respiratory Infections
- Microfluidic and Bio-sensing Technologies
- Bacterial Genetics and Biotechnology
- Biosensors and Analytical Detection
- 3D Printing in Biomedical Research
- Yersinia bacterium, plague, ectoparasites research
- Glycosylation and Glycoproteins Research
- Pancreatic and Hepatic Oncology Research
- Cancer-related molecular mechanisms research
- Escherichia coli research studies
- Reproductive tract infections research
- Metastasis and carcinoma case studies
- MicroRNA in disease regulation
- Chronic Lymphocytic Leukemia Research
- Antibiotic Resistance in Bacteria
- Immune cells in cancer
- Syphilis Diagnosis and Treatment
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- Cancer, Stress, Anesthesia, and Immune Response
- Bacillus and Francisella bacterial research
- Vector-borne infectious diseases
University of Missouri
2024
National Medical Products Administration
2022
State Administration of Traditional Chinese Medicine of the People's Republic of China
2022
Beijing University of Chinese Medicine
2022
Sixth Affiliated Hospital of Sun Yat-sen University
2018-2019
Sun Yat-sen University
2018-2019
Creatv MicroTech (United States)
2008-2018
Center for Biologics Evaluation and Research
2001-2005
Institut Pasteur
2001-2004
Wellcome Sanger Institute
2004
The association of historical plague pandemics with Yersinia pestis remains controversial, partly because the evolutionary history this largely monomorphic bacterium was unknown. microevolution Y. therefore investigated by three different multilocus molecular methods, targeting genomewide synonymous SNPs, variation in number tandem repeats, and insertion IS 100 elements. Eight populations were recognized we propose an tree for these populations, rooted on pseudotuberculosis . invokes over...
Circulating tumor cells (CTCs) disseminated into peripheral blood from a primary, or metastatic, can be used for early detection, diagnosis and monitoring of solid malignancies. CTC isolation by size exclusion techniques have long interested researchers as simple broad based approach, which is methodologically diverse use in both genomic protein detection platforms. Though variety these microfiltration systems are employed academically commercially, the limited ability to easily alter...
Natural sequence variation was investigated among serogroup A subgroup IV‐1 Neisseria meningitidis isolated from diseased patients and healthy carriers in The Gambia, West Africa. frequencies of DNA import were analysed by sequencing fragments four linked genes encoding the immunogenic outer membrane proteins TbpB (transferrin binding protein B) OpaA (an adhesin) plus two housekeeping enzymes. Seventeen foreign tbpB alleles independently imported into 98 strains tested, apparently due to...
The genetic variability at six polymorphic loci was examined within a global collection of 502 isolates subgroup III, serogroup A Neisseria meningitidis. Nine "genoclouds" were identified, consisting genotypes that isolated repeatedly plus 48 descendent rarely. These genoclouds have caused three pandemic waves disease since the mid-1960s, most recent which imported from East Asia to Europe and Africa in mid-1990s. Many are escape variants, resulting positive selection we attribute herd...
Abstract Long noncoding RNAs (lncRNAs) play a critical role in the initiation and progression of colorectal cancer (CRC), but little is known about function lncRNAs liver metastasis (CLM). This study was designed to identify specific correlating CRC, further assess their clinical value. Seventeen patients with primary CRC lesions, adjacent normal mucosa, synchronous metastases lesions were divided into discovery set (six patients) test (11 patients). Transcriptome sequencing (RNAseq) used...
Metastatic disease is the most important factor in determining survival of sarcoma patients. Since metastasis predominantly hematogenous, we hypothesized that detection and quantification circulating tumor cells (CTCs) could reflect response to therapy risk metastatic relapse. We evaluated presence CTCs using a novel animal model blood patients with high grade sarcomas utilizing CellSieve™ size-based low pressure microfiltration system. Sarcoma were identified based on antibody staining...
The chemoresistance of 5-fluorouracil (5-FU) limited the application chemotherapy in colorectal cancer (CRC) treatment. Herein, we aimed to uncover potential mechanism behind 5-FU resistance CRC cells.The abundance long noncoding RNA urothelial carcinoma associated 1 (lncRNA UCA1), microRNA-23b-3p (miR-23b-3p) and zinc finger protein 281 (ZNF281) was measured by quantitative real-time polymerase chain reaction (qRT-PCR) tissues cells. Western blot conducted examine autophagy-related...
Previous data have indicated that the opc gene encoding an immunogenic invasin is specific to Neisseria meningitidis (Nm) and lacking in gonorrhoeae (Ng). The presented here show Nm Ng both contain two paralogous opc-like genes, opcA, corresponding former gene, (psi)opcB, a pseudogene. predicted OpcA OpcB proteins possess transmembrane regions with conserved non-polar faces but differ extensively four of five surface-exposed loops. Gonococcal was expressed weakly under vitro conditions, it...
An all-in-one Circulating Tumor Cell (CTC) isolation platform with streamlined multiplex integration of proteomic and genomic clinical testing.
Circulating tumor cells (CTCs), epithelial-mesenchymal transition (EMT) cells, as well a number of circulating cancer stromal (CStCs) are known to shed into the blood patients. Individually, and together, these provide biological clinical information about cancers. Filtration is method used isolate all while eliminating red white from whole peripheral blood. We have previously shown that accurate identification cell types paramount proper assessment by describing overlapping phenotypes CTCs...
Abstract Molecular analysis of 103 serogroup A Neisseria meningitidis strains isolated in Moscow from 1969 to 1997 showed that four independent clonal groupings were responsible for successive waves meningococcal disease. An epidemic the mid-1970s was caused by genocloud 2 subgroup III, possibly imported China. Subsequent endemic disease through early 1990s X and then VI, which has also elsewhere Eastern Europe. 1996 part pandemic spread Asia 8 III. Recent may represent an warning these...
Molecular analysis of 103 serogroup A Neisseria meningitidis strains isolated in Moscow from 1969 to 1997 showed that four independent clonal groupings were responsible for successive waves meningococcal disease. An epidemic the mid-1970s was caused by genocloud 2 subgroup III, possibly imported China. Subsequent endemic disease through early 1990s X and then VI, which has also elsewhere eastern Europe. 1996 part pandemic spread Asia 8 III. Recent may represent an warning these bacteria...
Lipooligosaccharide (LOS) is a major virulence factor of the pathogenic Neisseria. Nine lgt genes at three chromosomal loci (lgt-1, 2, 3) encoding glycosyltransferases responsible for biosynthesis LOS oligosaccharide chains were examined in 26 Neisseria meningitidis, 51 gonorrhoeae and 18 commensal strains. DNA hybridization, PCR nucleotide sequence data compared to previously reported genes. Analysis genetic organization revealed that N. lgt-1 lgt-3 hypervariable genomic regions, whereas...
Cryopreserved peripheral blood mononuclear cells (PBMCs) are commonly collected in biobanks. However, little data exist regarding the preservation of tumor-associated cryopreserved collections. The objective this study was to determine feasibility using CellSieve™ microfiltration assay for isolation circulating tumor (CTCs) and cancer-associated macrophage-like (CAMLs) from PBMC samples. Blood samples spiked with breast (MCF-7), prostate (PC-3), renal (786-O) cancer cell lines were used...
Abstract While previous studies have shown that the number of circulating tumor cells (CTCs) alone is not sufficient to reflect progression and cyclooxygenase‐2 (COX‐2) expression correlated with colorectal cancer (CRC) metastasis, COX‐2 status its potential functions in CTCs CRC patients are unknown. Here, epithelial‐mesenchymal transition (EMT) phenotype‐based subsets were identified their clinical values assessed 91 patients. enumerated peripheral blood (epithelial [eCTCs], mesenchymal...
ABSTRACT Serogroup C Neisseria meningitidis belonging to the electrophoretic type (ET) ET-15, a variant of ET-37, is endemic in Canada. Like other serogroup ET-37 meningococci, ET-15 strains are usually found carry serotype and serosubtype antigens 2a:P1.5,2. In 2001, sudden increase number cases meningococcal disease Quebec, Canada, was caused by an antigenic strain. This carries unique marker P1.7,1. Strains C:2a:P1.7,1 meningococci were not isolated Canada large numbers prior...
Objective: This study aimed to identify the changes of miRNAs in colorectal cancer (CRC) complicated with diabetes mellitus (DM) (CRC + DM) tissues and their potential effects. Methods: The CRC DM were determined by miRNA microarray. expression levels miR-99a 40 clinical specimens 6 cell lines qRT-PCR. capacity for induce proliferation invasion was examined miR-99a-overexpressing HCT-116 cells. relative mTOR mRNA protein qRT-PCR Western blotting, respectively, cells transfected miR-99a. dual...
Molecular characterization of disseminated tumor cells (DTCs) in the bone marrow (BM) breast cancer (BC) patients has been hindered by their rarity. To enrich for these using an antigen-independent methodology, we have evaluated a size-based microfiltration device combination with several downstream biomarker assays.BM aspirates were collected from healthy volunteers or BC patients. Healthy BM was mixed specified number to calculate recovery and fold enrichment microfiltration. Specimens...
ABSTRACT The porin gene ( porB ) of Neisseria gonorrhoeae encodes the major outer membrane protein identified as PI or Por. To examine utility por variable-region (VR) typing, from 206 isolates was characterized by using oligonucleotide probes in a checkerboard hybridization assay that identifies sequence types five VRs both PIA and PIB alleles. strains represented temporally geographically distinct isolates, large cluster, epidemiologically linked partner collection disseminated gonococcal...
The motor behavior in patients with lumbar intervertebral disc degeneration (IDD) and animal models should be changed due to pain. However, there does not seem a strong correlation between IDD behavior. Therefore, it is necessary understand the age-related IDD.