A5110029359- Carbohydrate Chemistry and Synthesis
- Polyamine Metabolism and Applications
- Enzyme Structure and Function
- Biochemical and Molecular Research
- Mosquito-borne diseases and control
- Bacillus and Francisella bacterial research
- Viral Infections and Vectors
- Mass Spectrometry Techniques and Applications
- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Herpesvirus Infections and Treatments
- Bacterial Identification and Susceptibility Testing
- SARS-CoV-2 and COVID-19 Research
- Bacteriophages and microbial interactions
- Influenza Virus Research Studies
- Photodynamic Therapy Research Studies
- Long-Term Effects of COVID-19
- Plant tissue culture and regeneration
- Neural dynamics and brain function
- Cytomegalovirus and herpesvirus research
- Microbial Metabolites in Food Biotechnology
- Porphyrin Metabolism and Disorders
- vaccines and immunoinformatics approaches
- Blood groups and transfusion
- Microbial Inactivation Methods
Naval Medical Research Command
2019-2024
Jackson Foundation
2022
Henry M. Jackson Foundation
2020-2022
Science and Engineering Services (United States)
2011
Creatv MicroTech (United States)
2005-2008
University of Michigan
2000-2004
Center for Biologics Evaluation and Research
2004
Wayne State University
2003
The Ohio State University
1997-1999
COVID-19, caused by the SARS-CoV-2 virus, can lead to widespread neurological complications, including cognitive deficits and neurodegenerative symptoms, even in absence of significant structural brain abnormalities. The potential neuroprotective effects vaccination remain underexplored. Here, we demonstrate a psoralen-inactivated vaccine non-human primate model using resting-state magnetoencephalography (MEG), non-invasive neurophysiological recording technique with sub-millisecond temporal...
Dengue fever, caused by dengue viruses (DENV 1-4) is a leading cause of illness and death in the tropics subtropics. Therefore, an effective vaccine urgently needed. Currently, only available licensed chimeric live attenuated that shows varying efficacy depending on serotype, age baseline DENV serostatus. Accordingly, seronegative adults, children all ages immunocompromised individuals still We are currently researching use psoralen to develop inactivated tetravalent vaccine. Unlike...
Escherichia coli NeuNAc (N-acetylneuraminic acid) synthase catalyses the condensation of PEP (phosphoenolpyruvate) and ManNAc (N-acetylmannosamine) to form is encoded by neuB gene. Campylobacter jejuni has three genes, one which very similar E. We have characterized C. neuraminic acid with respect acylamino sugar specificity stereochemistry condensation. determined for N-acetylmannosamine, N-butanoylmannosamine, N-propionoylmannosamine N-pentanoylmannosamine. find that, although this enzyme...
COVID-19 caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has significantly impacted public health and the economy worldwide. Most of currently licensed vaccines act inhibiting receptor-binding function SARS-CoV-2 spike protein. The constant emergence variants resulting from mutations in domain (RBD) leads to vaccine immune evasion underscores importance broadly acting vaccines. Inactivated whole virus can elicit broader responses multiple epitopes several antigens help...
The development of a safe and effective vaccine to protect against COVID-19 is global priority due the current high SARS-CoV-2 infection rate. Currently, there are over 160 candidates at clinical or pre-clinical stages development. Of these, only three whole-virus produced using β-propiolactone formalin inactivation. Here, we prepared (SARS-CoV-2 PsIV) novel psoralen inactivation method evaluated its immunogenicity in mice two different adjuvants, alum Advax-2. We compared PsIV DNA vaccines...
Escherichia coli 3-deoxy-d-manno-octulosonate 8-phosphate (KDO8-P) synthase is able to utilize the five-carbon phosphorylated monosaccharide, 2-deoxyribose 5-phosphate (2dR5P), as an alternate substrate, but not d-ribose (R5P) nor four carbon analogue d-erythrose 4-phosphate (E4P). However, E. KDO8-P in presence of either R5P or E4P catalyzes rapid consumption approximately 1 mol PEP per active site, after which slows a negligible measurable rate. The mechanism this abortive utilization was...
An open reading frame, encoding for KDOPS (3-deoxy-D-manno-octulosonate 8-phosphate synthase), from Arabidopsis thaliana was cloned into a T7-driven expression vector. The protein overexpressed in Escherichia coli and purified to homogeneity. Recombinant A. KDOPS, solution, displays an apparent molecular mass of 76 kDa subunit 31.519 kDa. Unlike previously studied bacterial KDOPSs, which are tetrameric, appears be dimer solution. optimum temperature the enzyme is 65 °C pH 7.5, with broad...
The five-carbon phosphorylated monosaccharide analogues, d-arabinose 5-phosphate, d-ribose and 2-deoxy-d-ribose were separately condensed with (Z)- (E)-[3-2H]-phosphoenolpyruvate (PEP) in the presence of Escherichia coli 3-deoxy-d-arabino-heptulosonate 7-phosphate (DAH 7-P) synthase (phe) to give case (Z)-[3-2H]-PEP (3S)-[3-2H]-3-deoxy-d-manno-octulosonate 8-phosphate, (3S)-[3-2H]-3-deoxy-d-altro-octulosonate (3S)-[3-2H]-3,5-dideoxy-d-altro-octulosonate respectively, whereas incubation...
ABSTRACT Homogeneous, recombinant 3-deoxy- d - manno -octulosonate 8-phosphate synthase from Neisseria gonorrhoeae is shown to catalyze the formation of phosphoenolpyruvate and -arabinose 5-phosphate as determined 1 H-nuclear magnetic resonance analysis product. This enzyme does not condensation -erythrose 4-phosphate form ribo -heptulosonate 7-phosphate, was previously reported (P. S. Subramaniam, G. Xie, T. Xia, R. A. Jensen, J. Bacteriol. 180:119–127, 1998).
Traditional blood sampling by venipuncture is cumbersome and relatively expensive. Dried spot (DBS) desirable because of its ease sample collection, transportation storage. It has been used in clinical diagnosis but not thoroughly studied for the potential use to assess immune status individuals following natural infection or preventive vaccination. The goal this study was compare DBS traditional samplings detection antibodies vaccinated against measles, hepatitis A, tetanus, influenza...
Dengue fever, caused by any of four dengue viruses (DENV1-4), is a major global burden. Currently, there no effective vaccine that prevents infection in naïve populations. We tested the ability two novel adjuvants (Advax-PEI and Advax-2), using aluminum hydroxide (alum) as control, to enhance immunogenicity formalin- or psoralen-inactivated (PIV PsIV) DENV2 vaccines mice. Mice were vaccinated on days 0 30, serum samples collected 60, 90, 101. Neutralizing antibodies determined...
Vaccine immunogenicity is affected by a variety of factors. Melatonin has been reported to affect immune responses vaccines and infection. This was randomized open-label trial – in which adults scheduled receive the influenza vaccine were 5 mg melatonin or control evaluate effect post-vaccination on humoral (hemagglutination-inhibition assays, HAI) cellular (FluoroSpot) vaccine-specific cytokine 14–21 days post-vaccination. A total 108 participants (melatonin treatment group: 53; 55)...
Neisseria gonorrhoeae 3-deoxy-d-arabino-heptulosonate 7-phosphate (DAH 7-P) synthase catalyzes an aldol-type condensation between d-erythrose 4-phosphate and phosphoenolpyruvate (PEP) to form not 3-deoxy-d-ribo-heptulosonate 7-phosphate. Similar the Escherichia coli enzyme, N. DAH 7-P condenses d-arabinose 5-phosphate with PEP give 3-deoxy-d-manno-octulosonate 8-phosphate. Therefore, stereochemistry of reaction catalyzed by at C1 phosphorylated monosaccharide is same as that for E. namely,...
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