Abstract Background Cell division cycle associated 3 (CDCA3), part of the Skp1-cullin-F-box (SCF) ubiquitin ligase, refers to a trigger mitotic entry and mediates destruction mitosis inhibitory kinase. Little is known about relevance CDCA3 human malignancy including oral squamous cell carcinoma (OSCC). We aimed characterize expression state function in OSCC. Methods evaluated mRNA protein both OSCC-derived lines primary OSCCs performed functional analyses cells using shRNA system. Results...

BackgroundKinesin family member 4A (KIF4A), a microtubule-based motor protein, was implicated in regulation of chromosomal structure and kinetochore microtubule dynamics. Considering the functions KIF4A, we assumed that KIF4A is involved progression oral squamous cell carcinomas (OSCCs) via activation spindle assembly checkpoint (SAC). However, little known about relevance behavior OSCC. We investigated expression status its functional mechanisms MethodsThe levels seven OSCC-derived cells...

Adenosine A2b receptor (ADORA2B) encodes an adenosine that is a member of the G protein-coupled superfamily. This integral membrane protein stimulates adenylate cyclase activity in presence adenosine. Little known about relevance ADORA2B to human malignancy including oral squamous cell carcinoma (OSCC). We aimed characterize expression state and function OSCC. The levels nine OSCC-derived cells were analyzed by quantitative reverse transcriptase-polymerase chain reaction immunoblotting...

Dermatopontin (DPT), a component of the extracellular matrix (ECM), is involved in promotion cellular adhesion and ECM assembly activities. However, role DPT pathogenesis carcinoma unclear. We evaluated expression human oral cancer its possible roles including invasiveness. first investigated mRNA protein status squamous cell (OSCC)-derived cells. Real-time quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) immunoblotting analysis detected frequent downregulation...

Cell division cycle associated 2 (CDCA2) recruits protein phosphatase 1 to chromatin antagonize activation of ataxia telangiectasia mutated (ATM)-dependent signal transduction. ATM kinase plays a critical role in the DNA damage response and its phosphorylation cascade inhibit p53-MDM2 interaction, which releases p53 induce p21 G1 cell-cycle arrest. However, relevance CDCA2 human malignancy including oral squamous cell carcinoma (OSCC) is unknown. In current study, we found that expression...

Annexins are calcium and phospholipid binding proteins that form an evolutionary conserved multigene family. Considerable evidence indicates annexin A10 (ANXA10) is involved in tumoral progression, although little known about its role human oral carcinogenesis. In this study, we investigated the involvement of ANXA10 squamous cell carcinoma (OSCC).ANXA10 mRNA protein expressions were assessed by quantitative reverse transcriptase polymerase chain reaction immunoblotting, conducted a...

Inflammatory abnormalities have been implicated in the pathogenesis of various human diseases, including cancer. Interleukin-1 receptor antagonist (IL1RN) is a potent anti-inflammatory molecule that modulates biological activity proinflammatory cytokine, interleukin-1. The aim this study was to examine expression IL1RN oral squamous cell carcinomas (OSCCs), and determine its clinical significance. Expression levels matched normal tumor specimens from 39 OSCCs were evaluated using real-time...

Matrix metalloproteinases (MMP) play an important role in tumor cell invasion and metastasis. We examined the expression of MMPs hepatocellular carcinoma (HCC) to determine whether they may help indicate progression HCC patient outcome. The MMP-1, -2, -7, -9, MT1-MMP were determined 37 pairs adjacent non-tumor tissue specimens by semiquantitative reverse transcription polymerase chain reaction (RT-PCR). (T/N) ratios for mRNA each MMP compared with clinicopathological findings two-year...

LIM and SH3 protein 1 (LASP-1) is a specific focal adhesion involved in several malignant tumors. However, its role oral squamous cell carcinoma (OSCC) unknown. The aim of this study was to characterize the molecular status/mechanism LASP-1 OSCC.We evaluated mRNA expressions OSCC-derived lines primary OSCCs. Using an shRNA system, we analyzed effect on biology function OSCC lines, HSC-3 Ca9-22. cells also were subcutaneously injected evaluate tumor growth vivo. Data by Fisher's exact test or...

The aim of the present study was to identify a target molecule that could predict efficacy radiotherapy in oral squamous cell carcinoma (OSCC). We used DNA microarray analysis differences gene expression after X-ray irradiation. compared profiles between (8 Gy)-irradiated Ca9-22 cells (an OSCC-derived line) and unirradiated cells. A total 167 genes with 2-fold higher level induced by irradiation were identified. Lipocalin-2 (LCN2) had greatest increase irradiation, it categorized network has...

While circulating tumor-derived molecules have been identified in a variety of malignant tumors, it is sometimes difficult to detect the molecular targets due lower serum concentration. We report that evaluation mitochondrial DNA (mtDNA) seems novel efficiency for detecting tumoral micrometastasis. In murine xenografting human oral cancer cells, mtDNAs could be quantitatively detected from multiple organs and blood samples whereas nucleic DNAs not. also determined if this mtDNA test was...

Persephin (PSPN) is a neurotrophic factor of the glial cell line-derived (GDNF) family that promotes survival multiple populations neurons. Little known about relevance PSPN in human malignancy including oral squamous carcinoma (OSCC). This study was undertaken to evaluate mRNA and protein expression by analyzing cellular proliferation cycle knockdown cells vitro. were significantly (P < 0.05) up-regulated OSCC-derived compared with normal keratinocytes (n = 7). Cellular decreased reduced...

The four and a half LIM domains 1 (FHL1) gene has been related to carcinogenesis. However, the expression status of FHL1 in human oral squamous cell carcinoma (OSCC) remains unclear detailed mechanism silencing is poorly understood. aim this study was examine level its regulatory OSCCs. Quantitative reverse-transcriptase-polymerase chain reaction (PCR) western blotting showed significant downregulation all OSCC-derived lines (Sa3, HSC-2, HSC-3, HSC-4, HO-1-u-1, HO-1-N-1, KON Ca9-22) compared...

Abstract We previously reported that human squamous cell carcinoma ( SCC ) lines refractory to cis‐diaminedichloro‐platinum II (cisplatin [ CDDP ]) had significant upregulation of the phosphodiesterase 3B gene PDE3B ), suggesting inhibiting suppresses resistance. sh RNA ‐mediated depletion in ‐resistant cells derived from and Hela induced sensitivity inhibited tumor growth with elevated cyclic GMP induction resulting multidrug‐resistant molecule, but this did not occur...

Dickkopf-1 (Dkk1), a negative regulator of the Wnt signaling pathway, is implicated in tumorigenesis several types cancer. The purpose this study was to determine involvement Dkk1 oral squamous cell carcinoma (OSCC). We found that frequently upregulated OSCC-derived lines and primary OSCCs compared with normal counterparts. Unexpectedly, Dkk1-positive cases were correlated significantly (P<0.05) low risk regional lymph node metastasis. also cellular migration invasiveness increased knockdown...

We consider the problem of correct motion planning for T-intersection merge-ins arbitrary geometry and vehicle density. A merge-in support system has to estimate chances that a gap between two consecutive vehicles can be taken successfully. In contrast previous models based on heuristic size rules, we present an approach which optimizes integral risk situation using parametrized velocity ramps. It accounts risks from curves all involved (front rear paths) with so-called survival analysis....

No definitive therapy exists to treat human metastatic tumors. We reported previously that down-regulation of Lin-7C is essential for metastasis squamous cell carcinomas (hSCCs). In this study, we investigated the chemical restoration expression and demonstrated its effectiveness suppressing potential in cancer cells. Ingenuity Pathway Analysis (IPA) identified candidate agents, i.e., apomorphine, caffeine, risperidone, quetiapine mirtazapine. Among them, mirtazapine, an antagonist HTR2C,...