The 47,000-D collagen-binding glycoprotein, heat shock protein 47 (HSP47), is a stress-inducible localized in the ER of collagen-secreting cells. location and activity this led to speculation that HSP47 might participate collagen processing. Chemical crosslinking studies were used test hypothesis both before after perturbation procollagen association with was demonstrated using cleavable bifunctional reagents. shown be coprecipitated by treatment intact cells anti-HSP47 or anticollagen...

HSP47 is a collagen-binding stress protein and assumed to act as collagen-specific molecular chaperone during the biosynthesis secretion of procollagen in living cell. The synthesis has been reported correlate with that collagen several cell lines. We examined expression mRNA progression carbon tetrachloride (CCl4)-induced liver fibrosis rats. Northern blot analysis revealed was markedly induced parallel alpha 1(I) 1(III) mRNAs. By situ hybridization, distribution transcripts similar...

Heat shock proteins of chick embryo fibroblasts were analyzed on SDS polyacrylamide gradient gels and found to include not only three previously well-characterized 25,000, 73,000, 89,000 D, but also a 47,000-D protein. Two-dimensional gel electrophoresis revealed that this protein was unusually basic (pI = 9.0) corresponded recently characterized, major gelatin- collagen-binding The induction synthesis membrane glycoprotein after heat stress particularly apparent in preparations isolated by...

Transcriptional activation of human heat shock protein (HSP) genes by or other stresses is regulated the a factor (HSF). Activated HSF posttranslationally acquires DNA-binding ability. We previously reported that quercetin and some flavonoids inhibited induction HSPs in HeLa COLO 320DM cells, derived from colon cancer, at level mRNA accumulation. In this study, we examined effects on HSP70 promoter-regulated chloramphenicol acetyltransferase (CAT) activity binding to element (HSE) gel...

Heat shock protein 47 (HSP47), a collagen-specific stress protein, has been postulated to be molecular chaperone localized in the ER. We previously demonstrated that HSP47 transiently associated with newly synthesized procollagen ER (Nakai, A., M. Satoh, K. Hirayoshi, and Nagata. 1992. J. Cell Biol. 117:903-914). In present work, we examined location where binds dissociates from within cells, whether associates nascent single polypeptide chains and/or mature triple-helix procollagen. This...

A major collagen-binding heat shock protein of molecular mass 47,000 D was found to bind collagen by a pH-dependent interaction; binding abolished at pH 6.3. Native 47-kD could therefore be purified from chick embryo homogenates in milligram quantities gelatin-affinity chromatography and gentle acidic elution. Rat monoclonal rabbit polyclonal antibodies were generated against the protein. Immunofluorescence microscopy cultured fibroblasts with these revealed bright, granular perinuclear...

We report the isolation and characterization of a cDNA clone encoding HSP47, transformation-sensitive heat shock protein that binds to collagen. A library was prepared from total RNA isolated heat-shocked chicken embryo fibroblasts screened by using oligonucleotide mixtures on basis N-terminal amino acid sequence biochemically purified HSP47. The insert contained 3,278 bp, which encoded 15-amino-acid signal peptide mature coding region consisting 390 residues; it also included part 5'...

Glycoprotein folding and degradation in the endoplasmic reticulum (ER) is mediated by ER quality control system. Mannose trimming plays an important role forming specific N-glycans that permit recognition sorting of terminally misfolded conformers for ERAD (ER-associated degradation). The EDEM (ER enhancing α-mannosidase-like protein) subgroup proteins belonging to Class I α1,2-mannosidase family (glycosylhydrolase 47) has been shown enhance ERAD. We recently reported overexpression EDEM3...

The thermodynamic stabilities of semiclathrate hydrates were investigated in the tetra-n-butyl ammonium chloride (TBAC) aqueous solution (mole fraction TBAC is 0.0323) + H2, N2, CH4, CO2, and C2H6 systems. dissociation temperature each hydrate higher than that simple (hydration number 30) except for C2H6-containing system whole pressure region under present experimental conditions. Isobaric temperatures CO2 increase this order. temperature−pressure projection indicated structural transition...

Based on information of the nucleotide sequence obtained from rat genome clones, a new CYP3A (CYP3A62) cDNA was isolated library liver. The CYP3A62 1746 base pairs (bp) in length, which included 1491 bp an open reading frame and 93 209 respective 5′- 3′-noncoding regions. Amino acid deduced shared highest similarity with CYP3A9 (79.9%) among human forms previously reported. mRNA protein were consistently detected small intestines as well livers. major form both sexes but female-predominant...

Data from animal experiments show that the radiation-related risk of cancer decreases if dose rate is reduced, even though cumulative unchanged (i.e., a dose-rate effect); however, underlying mechanism not well understood. To explore factors effect observed in experimental rat mammary carcinogenesis, we developed mathematical model accounts for cellular dynamics during and then examined whether predicts incidence. A multistage carcinogenesis involving radiation-induced cell death mutagenesis...

The synthesis of a major collagen-binding heat-shock protein molecular weight 47,000 (hsp47) was shown previously to be decreased after transformation chick embryo fibroblasts by Rous sarcoma virus (Nagata, K., and Yamada, K. M. (1986) J. Biol. Chem. 261, 7531-7536; Nagata, Saga, S., Cell 103, 223-229). In this paper, further study demonstrated that the total amount hsp47 are also factor three in BALB/3T3 cells transformed simian 40 (SV40). Higher observed for resting state compared those...

Quantitative and qualitative changes in cellular actin were followed during differentiation of a myeloid leukemia cell line, namely Ml, which was inducible with conditioned medium (CM). During 3 d incubation CM, when the Ml cells differentiated to macrophages lost their mitotic activity, content, F-actin ratio total actin, synthesis showed an increase. A greater difference before after found ability G-actin polymerize. Actin harvested from CM-treated polymerize, depending on increased...

The development of an in vivo system for investigating osteoclast differentiation is important because molecular events occurring can be observed during the authentic osteoclasts. In adjuvant arthritic rats, experimental model human rheumatoid arthritis, extensive bone resorption distal diaphysis tibia. area resorption, it always accompanied with clusters numerous multinucleated giant cells (MGCs) as well bone-resorbing Here we characterized morphological properties these MGCs use...

The mechanism of osteoclast-like cell formation induced by periodontopathic bacterium Actinobacillus actinomycetemcomitans Y4 (serotype b) capsular-polysaccharide-like polysaccharide (capsular-like polysaccharide) was examined in a mouse bone marrow culture system. When cells were cultured with A. capsular-like for 9 days, many multinucleated formed. showed several characteristics osteoclasts, including tartrate-resistant acid phosphatase (TRACP) and the ability to resorb calcified dentine....

Journal Article Molecular Cloning and Expression of an Amine Sulfotransferase cDNA: A New Gene Family Cytosolic Sulfotransferases in Mammals Get access Kouichi Yoshinari, Yoshinari *Division Drug Metabolism Toxicology, Faculty Pharmaceutical Sciences, Tohoku UniversityAramaki-Aoba, Aoba-ku, Sendai 980-8578 Search for other works by this author on: Oxford Academic PubMed Google Scholar Kiyoshi Nagata, Nagata 2 2To whom correspondence should be addressed. Phone: 81-22-217-6830, Fax:...

1. The effects of chemical agents on the metabolism antihistamine drug astemizole were investigated to evaluate drug-drug interactions. 2. Chemical inhibitors O-demethylation screened using small intestinal and liver microsomes from rabbit as an animal model for first-pass humans. In intestine, was clearly inhibited by ebastine, arachidonic acid, alpha-naphthoflavone, ketoconazole, tranylcypromine, troglitazone terfenadine. 3. humans, these also reduced microsomal in both intestine liver....

The mouse HSP47 gene consists of six exons separated by five introns. Three cDNAs differing only in their 5' noncoding regions have been reported. One these alternatively spliced mRNAs was detected after heat shock, which caused an alternative splice donor site selection. Other stress inducers, including amino acid analog and sodium arsenite, had no effect on the splicing. mRNA, 169 nucleotides longer region compared to mRNA transcribed non-heat shock conditions, efficiently translated under...