A5065428373- Effects of Radiation Exposure
- Radiation Therapy and Dosimetry
- Radiation Dose and Imaging
- Cancer Cells and Metastasis
- Cancer-related Molecular Pathways
- Carcinogens and Genotoxicity Assessment
- Cancer Risks and Factors
- DNA Repair Mechanisms
- Cancer, Hypoxia, and Metabolism
- Cancer Research and Treatments
- Cancer Genomics and Diagnostics
- Nutrition, Genetics, and Disease
- Epigenetics and DNA Methylation
- Lung Cancer Treatments and Mutations
- Genetic factors in colorectal cancer
- Cancer Treatment and Pharmacology
- Estrogen and related hormone effects
- MicroRNA in disease regulation
- Radiation Effects and Dosimetry
- BRCA gene mutations in cancer
- Boron Compounds in Chemistry
- Ubiquitin and proteasome pathways
- Breast Cancer Treatment Studies
- Growth Hormone and Insulin-like Growth Factors
- Advanced Radiotherapy Techniques
National Institutes for Quantum Science and Technology
2016-2025
Radiation Effects Research Foundation
2018-2024
National Center for Global Health and Medicine
2023
Tokyo Metropolitan University
2016-2022
Fukushima Medical University
2022
University of Manchester
2015
Zero to Three
2013
Institute for Biotechnology and Bioengineering
2012
Cancer Research UK Cambridge Center
2012
Tokushima Bunri University
2012
Germline-mutation in BRCA1 tumor suppressor gene is an established risk for carcinogenesis not only females but also males. Deficiency the repair of DNA double-strand breaks hypothesized as a responsible mechanism carcinogenesis. However, supporting data insufficient both mutation spectra cancers patients with germline-mutation and murine knockout/knock-in models Brca1 haploinsufficiency. Furthermore, information on driving force toward carriers lacking. Here we applied Fenton reaction-based...
Data from animal experiments show that the radiation-related risk of cancer decreases if dose rate is reduced, even though cumulative unchanged (i.e., a dose-rate effect); however, underlying mechanism not well understood. To explore factors effect observed in experimental rat mammary carcinogenesis, we developed mathematical model accounts for cellular dynamics during and then examined whether predicts incidence. A multistage carcinogenesis involving radiation-induced cell death mutagenesis...
Abstract BRCA1 is one of the causative genes for hereditary breast and ovarian cancer syndrome with a high risk early‐onset cancer. Whereas olaparib (OLA), an inhibitor poly‐ADP‐ribose polymerase, has been applied as adjuvant therapy to those patients, its effect on reproductive function remains unelucidated. Recently, rat model (MUT; Brca1 (L63X/+) mutation) mimicking human pathogenic variant established. Using this model, we evaluated effects OLA in comparison wild‐type (WT) rats. MUT...
With the increase in number of long-term cancer survivors worldwide, there is a growing concern about risk secondary cancers induced by radiotherapy. Epigenetic modifications genes associated with carcinogenesis are attractive targets for prevention owing to their reversible nature. To identify possible changes functionally relevant DNA methylation patterns mammary carcinomas radiation exposure, we performed microarray-based global and expression profiling γ-ray-induced rat normal glands....
Fluorescence imaging is a critical tool to understand the spatial distribution of biomacromolecules in cells and vivo, providing information on molecular dynamics interactions. Numerous valuable insights into biological systems have been provided by specific detection various species. However, molecule-selective often hampered background fluorescence, such as cell autofluorescence fluorescence leakage from molecules stained other dyes. Here we describe method for all-optical selective...
Recent studies have identified interstitial deletions in the cancer genome as a radiation-related mutational signature, although most of them do not fall on driver genes. Pioneering field indicated presence loss heterozygosity (LOH) spanning Apc subset sporadic and radiation-induced intestinal tumors ApcMin/+ mice, albeit with substantial which LOH was detected; whether copy number losses accompany such has also been unclear. Herein, we analyzed C3B6F1 mice that were either left untreated or...
The relative biological effectiveness (RBE) of neutrons depends on their physical nature (e.g., energy) and the context end points, materials). From perspective radiological protection, age is an important that influences radiation-related cancer risk, but very few studies have addressed its potential impact neutron effects. We therefore investigated influence effect accelerator-generated fast (mean energy, ∼2 MeV) in animal model breast carcinogenesis. Female Sprague-Dawley rats at 1, 3 7...
Women who are heterozygous for deleterious BRCA1 germline mutations harbor a high risk of hereditary breast cancer. Previous Brca1-heterozygous animal models do not recapitulate the cancer phenotype, and thus all currently used knockout adopt conditional, mammary-specific homozygous Brca1 loss or addition Trp53 deficiency. Herein, we report creation characterization novel mutant rat model harboring L63X mutation, which mimics founder mutation in Japan, through CRISPR-Cas9-based genome...
Age at exposure is a critical factor that influences the risk of radiation-induced leukemia, which arises from hematopoietic stem and progenitor cells. However, little known about effect age on radiation response these In this study, we examined cells in infant (1-week-old), juvenile (3-week-old), adult (8- 14-week-old) C3H/He mice, are susceptible to myeloid leukemia. We first observed an age-dependent increase radioresistance after vivo irradiation. vitro irradiation did not show any...
The RERF International Low-Dose Symposium was held on 5–6 December 2013 at the campus in Hiroshima, Japan, to discuss issues facing Life Span Study (LSS) and other low-dose studies. Topics included current status of risk detection, strategies for epidemiological statistical research, methods improve communication between epidemiologists biologists, radiological studies tools. Key points made by participants necessity pooling materials over multiple gain greater insight where data from single...
Cancer risk after exposure to ionizing radiation can vary between individuals and populations, but the impact of factors governing those variations is not well understood. We previously conducted a series carcinogenesis experiments using rat model breast cancer, in which 1654 rats born 2002-2012 were exposed γ rays at various doses ages with or without non-radiation including high-fat diet, parity chemical carcinogens. herein reanalyze incidence data from these archival clarify effect age...
We examined the response of developing mouse intestine to X radiation using neonates (1 day postpartum), infants (2 weeks postpartum) and adults (7 postpartum). Irradiated adult small intestinal crypts displayed two waves apoptosis. The first wave peaked at 3 h was followed by a broad with peak persisting from 24 48 h. p53 expressed during but not second wave. For infant intestine, intensity approximately half that wave, for colon even smaller. In neonates, apoptosis delayed, peaking 6...
Abstract Childhood exposure to carcinogens renders a higher risk of breast cancer. The molecular mechanisms underlying cancer development after such are not, however, well understood. Here we examined how the mechanism relates age at ionizing radiation (IR) or carcinogen 1‐methyl‐1‐nitrosourea (MNU). Pre‐ and postpubertal (3‐ 7‐wk‐old, respectively) female Sprague–Dawley rats were whole‐body γ‐irradiated (2 Gy), injected intraperitoneally with MNU (20 mg/kg) left untreated autopsied 50 wk...
BRIP1 is a DNA helicase that directly interacts with the C-terminal BRCT repeat of breast cancer susceptibility protein BRCA1 and plays an important role in BRCA1-dependent repair damage–induced checkpoint control. Recent studies implicate as moderate/low-penetrance gene. However, phenotypic effects dysfunction its tumorigenesis remain unclear. To explore function acinar morphogenesis mammary epithelial cells, we generated BRIP1-knockdown MCF-10A cells by short hairpin RNA (shRNA)-mediated...