A5087748250- Neonatal Respiratory Health Research
- Glycosylation and Glycoproteins Research
- Heme Oxygenase-1 and Carbon Monoxide
- Cancer, Hypoxia, and Metabolism
- Fatty Acid Research and Health
- Asthma and respiratory diseases
- Neuroscience of respiration and sleep
- Alcohol Consumption and Health Effects
- Amyotrophic Lateral Sclerosis Research
- Proteoglycans and glycosaminoglycans research
- Cannabis and Cannabinoid Research
- Galectins and Cancer Biology
- HER2/EGFR in Cancer Research
- Pediatric health and respiratory diseases
- Medical Imaging and Pathology Studies
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Diet, Metabolism, and Disease
- Thermal Regulation in Medicine
- Fibroblast Growth Factor Research
- Hemoglobin structure and function
- RNA modifications and cancer
- Phagocytosis and Immune Regulation
- Pulmonary Hypertension Research and Treatments
- Monoclonal and Polyclonal Antibodies Research
- Immune Response and Inflammation
The University of Tokyo
2019-2023
Sapporo Medical University
2013-2020
Max Planck Society
2013
RIKEN Advanced Science Institute
2012
Keio University
1998-2012
Brigham and Women's Hospital
2008
Harvard University
2007-2008
Osaka University
2003-2007
Kyung Hee University
2007
Boston Children's Hospital
2007
Abstract —This study aimed to examine whether an elevated activity of heme oxygenase (HO)-1 in the tissue attenuates endothelial cell–leukocyte interactions microvessels vivo. When rats were pretreated with intraperitoneal injection hemin, HO-1 inducer, mesenteric tissues, including their microvessels, displayed a marked induction concurrent increase plasma concentrations bilirubin-IXα, product HO-catalyzed degradation protoheme IX. In these rats, oxidative stress such as superfusion H 2 O...
Whereas pneumonia is the most common cause of death and disability worldwide, cases spontaneously resolve. Mechanisms that promote resolution remain to be determined. Resolvin E1 (RvE1) an endogenous mediator displays proresolving actions in sterile inflammation. In this study, we developed a new model aspiration evaluate effect RvE1 on acute lung injury caused by acid subsequent bacterial challenge. Mice received hydrochloric into left followed enteric pathogen Escherichia coli. I.v....
We previously demonstrated that Siglec-15, a member of the Siglec family glycan-recognition proteins, is expressed on subset macrophages and preferentially recognizes sialyl-Tn (sTn) antigen, tumor-associated glycan structure. In this study, we report biological significance Siglec-15-mediated interaction between monocytes/macrophages cancer cells. Siglec-15 (TAMs) in various human tumor tissues. further its expression substantially elevated macrophage colony-stimulating factor-induced...
Choline supplies methyl groups for regeneration of methionine and the donor S-adenosylmethionine in liver. Here, we report that catabolism membrane phosphatidylcholine (PC) into water-soluble glycerophosphocholine (GPC) by phospholipase/lysophospholipase PNPLA8-PNPLA7 axis enables endogenous choline stored hepatic PC to be utilized metabolism. PNPLA7-deficient mice show marked decreases GPC, choline, several metabolites related cycle, accompanied various signs insufficiency, including growth...
Acrolein, a known toxin in tobacco smoke, might be involved atherogenesis. This study examined the effect of acrolein on expression cyclooxygenase-2 (COX-2) and prostaglandin (PG) production endothelial cells.Cyclooxygenase (COX)-2 induction by signal pathways were measured using Western blots, Northern immunofluorescence, ELISA, gene silencing, promoter assay. Colocalization COX2 acrolein-adduct was determined immunohistochemistry. Here we report that levels COX-2 mRNA protein are increased...
Section:ChooseTop of pageAbstract <<MATERIALS AND METHODSRESULTSDISCUSSIONReferencesCITING ARTICLES
Psoriasis is an inflammatory disorder characterized by keratinocyte hyper-proliferation and Th17-type immune responses. However, the roles of bioactive lipids regulation their biosynthesis in this chronic skin disease are not fully understood. Herein, we show that group IVE cytosolic phospholipase A2 (cPLA2 ε/PLA2G4E) plays a counterregulatory role against psoriatic inflammation producing anti-inflammatory lipid N-acylethanolamine (NAE). Lipidomics analysis mouse revealed NAE species...
This study aimed to examine whether livers overexpressing heme oxygenase (HO)-1 could alter the vascular resistance through vasorelaxing action of carbon monoxide (CO). The relationship among HO-1 expression, CO generation, and was assessed in perfused rat pretreated with hemin, an inducer HO-1. At 18 h after hemin treatment, displayed marked increases expression hepatocytes venous flux a reduction basal resistance. hemin-treated canceled by administration oxyhemoglobin, reagent trapping...
Oxidative stress has been implicated as a cause of various diseases such anaemia. We found that the SOD1 [Cu,Zn-SOD (superoxide dismutase)] gene deficiency causes anaemia, production autoantibodies against RBCs (red blood cells) and renal damage. In present study, to further understand role oxidative in autoimmune response triggered by deficiency, we generated mice had hSOD1 (human SOD1) transgene under regulation GATA-1 promoter, bred onto SOD1−/− background (SOD1−/−;hSOD1tg/+). The...
Cigarette smoke (CS) induces oxidative stress, which disables macrophage function. In this study, we examined whether Resolvin E1 (RvE1), a pro‐resolving mediator known to enhance functions, attenuates the damage of macrophages by CS extract (CSE) induced stress. RvE1 blocked p47phox translocation plasma membrane CSE in cell line, RAW264.7 cells, resulting suppression superoxide production. Furthermore, pretreatment cells with restored phagocytic activity and reduced death treatment CSE....
With the recent increasing use of nanoparticles, there is concern that they may become an environmental risk factor as airborne particles. However, impact these particles on susceptible subjects with predisposing lung disease have not been sufficiently elucidated. In present study, we investigated effects nanoparticles pulmonary inflammatory and fibrotic changes induced by intratracheal bleomycin (BLM) challenge in mice. Mice were intratracheally administered either vehicle, 14-nm carbon...
Nucleotide sugars are the donor substrates of various glycosyltransferases, and an important building block in N- O-glycan biosynthesis. Their intercellular concentrations regulated by cellular metabolic states including diseases such as cancer diabetes. To investigate fate UDP-GlcNAc, we developed a tracing method for UDP-GlcNAc synthesis use, GlcNAc utilization using (13)C6-glucose (13)C2-glucosamine, respectively, followed analysis mass isotopomers LC-MS. Metabolic labeling cultured cells...
This study examined the role of bilirubin in heme oxygenase (HO)-1-mediated amelioration mast cell (MC)-elicited inflammatory responses. Pretreatment rats with an intraperitoneal injection hemin, inducer HO-1, evolved a marked induction enzyme MCs. Intravital videomicroscopy revealed that hemin pretreatment attenuated compound 48/80-elicited degranulation MCs and resultant leukocyte adhesion venules. Superfusion biliverdin or bilirubin, but not carbon monoxide (CO), another product HO...
Among the phospholipase A2 (PLA2) superfamily, group IVA cytosolic PLA2 (cPLA2α) is currently attracting much attention as a central regulator of arachidonic acid (AA) metabolism linked to eicosanoid biosynthesis. Following cell activation, cPLA2α selectively releases AA, precursor variety eicosanoids, from phospholipids in perinuclear membrane compartments. cPLA2α-null mice display various phenotypes that could be largely explained by reduced signaling. In contrast, IVE cPLA2ε, another...
Efferocytosis is believed to be a key regulator for lung inflammation in chronic obstructive pulmonary disease. In this study we pharmacologically inhibited efferocytosis with annexin V and attempted determine its impact on the progression of emphysema mouse. We first demonstrated vitro vivo experiments using V, an inhibitor phosphatidylserine-mediated efferocytosis. then porcine pancreatic elastase (PPE)-treated mice. PPE-treated mice were instilled intranasally starting from day 8 until...
Amyotrophic lateral sclerosis (ALS) involves the progressive degeneration of motor neurons in spinal cord and cortex. It has been shown that 15-20% patients with familial ALS (FALS) have defects Sod1 gene encodes Cu, Zn-superoxide dismutase (SOD). To elucidate pathological role mutated Zn-SODs FALS, susceptibility mutants to glycation was examined. Mutated (G37R, G93A, I113T) related FALS wild type were produced a baculovirus/insect cell expression system. Glycated nonglycated proteins...
Abstract The extent to which defective innate immune responses contribute chronic obstructive pulmonary disease (COPD) is not fully understood. Pulmonary surfactant protein A (SP-A) plays an important role in regulating immunity the lungs. In this study, we hypothesised that cigarette smoke (CS) and its component acrolein might influence by affecting function of SP-A. Indeed, acrolein-modified SP-A was detected lungs mice exposed CS for 1 week. To further confirm finding, recombinant human...
Amyotrophic lateral sclerosis (ALS) involves the progressive degeneration of motor neurons in spinal cord and cortex. It has been shown that 15-20% patients with familial ALS (FALS) have defects Sod1 gene, which encodes Cu,Zn-superoxide dismutase (SOD). To elucidate pathological role mutated Cu,Zn-SOD, we examined issue whether Cu,Zn-SOD affects cell cycle. Mouse neuroblastoma Neuro-2a cells were transfected human wild-type or (G37R, G93A) Cu,Zn-SOD. Mutated, Cu,Zn-SOD-transfected exhibited...
Abstract Surfactant protein A (SP-A) is a multifunctional host defense collectin that was first identified as component of pulmonary surfactant. Although SP-A also expressed in various tissues, including the urinary tract, its innate immune functions nonpulmonary tissues are poorly understood. In this study, we demonstrated adherence uropathogenic Escherichia coli (UPEC) to bladder enhanced SP-A–deficient mice, which suggests plays an important role immunity against UPEC. To understand...