A5103116141- Amyotrophic Lateral Sclerosis Research
- Redox biology and oxidative stress
- Nitric Oxide and Endothelin Effects
- Parkinson's Disease Mechanisms and Treatments
- Cholesterol and Lipid Metabolism
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Alzheimer's disease research and treatments
- Neurogenetic and Muscular Disorders Research
- Iron Metabolism and Disorders
- Synthesis and Biological Evaluation
- Synthesis and Reactions of Organic Compounds
- Heme Oxygenase-1 and Carbon Monoxide
- Stress Responses and Cortisol
- Metal-Catalyzed Oxygenation Mechanisms
- Antioxidant Activity and Oxidative Stress
- Glutathione Transferases and Polymorphisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Trace Elements in Health
- Vitamin C and Antioxidants Research
- Prion Diseases and Protein Misfolding
- Hemoglobinopathies and Related Disorders
- Organ Transplantation Techniques and Outcomes
- Biomedical and Engineering Education
- Problem and Project Based Learning
- Adenosine and Purinergic Signaling
Hyogo Medical University
2013-2022
Saitama University
2014
Aichi Prefectural Government
2012
Juntendo University
1994-2011
Université Laval
2010
Osaka University
1996-2010
Seikagaku Corporation (Japan)
2010
Kyoto University
2010
RIKEN Advanced Science Institute
2010
National Center of Neurology and Psychiatry
2010
Although estrogen is known to activate endothelial nitric oxide synthase (eNOS) in the vascular endothelium, molecular mechanism responsible for this effect remains be elucidated. In studies of both human umbilical vein cells (HUVECs) and simian virus 40-transformed rat lung (TRLECs), 17β-estradiol (E2), but not 17α-E2, caused acute activation eNOS that was unaffected by actinomycin D specifically blocked pure receptor antagonist ICI-182,780. Treatment TRLECs HUVECs with 17β-E2 stimulated...
Copper-zinc superoxide dismutase (SOD1) plays a protective role against oxidative stress. On the other hand, recent studies suggest that SOD1 itself is major target of damage and has its own pathogenicity in various neurodegenerative diseases, including familial amyotrophic lateral sclerosis. Only human great ape SOD1s among mammals have highly reactive free cysteine residue, Cys(111), at surface molecule. The purpose this study was to investigate Cys(111) protein, by comparing...
Recently, the successful application of portal inflow modulation has led to renewed interest in use left lobe grafts adult-to-adult living donor liver transplantation (LDLT). However, data on hepatic hemodynamics supporting are limited, and optimal circulation for a graft is still unclear. We analyzed 42 consecutive LDLT cases without splenectomy or portocaval shunt. The mean actual volume (GV)/recipient standard (SLV) ratio was 39.8% ± 5.7% (median = 38.9%, range 26.1%-54.0%). GV/SLV less...
Abstract Background A subset of familial forms amyotrophic lateral sclerosis (ALS) are caused by mutations in the gene coding Cu/Zn-superoxide dismutase (SOD1). Mutant SOD1 proteins susceptible to misfolding and abnormally accumulated spinal cord, which is most severely affected ALS. It, however, remains quite controversial whether wild-type involved more prevalent sporadic ALS (sALS) cases without mutations. Methods Cerebrospinal fluid (CSF) from patients including sALS as well several...
Vaccinations targeting extracellular superoxide dismutase 1 (SOD1) mutants are beneficial in mouse models of amyotrophic lateral sclerosis (ALS). Because its misfolded nature, wild-type nonmetallated SOD1 protein (WT-apo) may have therapeutic application for vaccination various mutants. We compared the effects WT-apo to those a G93A vaccine low-copy transgenic mice. Both vaccines induced antibody against and significantly delayed disease onset with saline/adjuvant controls. extended life...
Modification of cell surface oligosaccharides by reactive oxygen species (ROS) and the biological effect such modifications on adhesion were investigated. Treatment HL60, a human promyelocyte leukemia line, with ROS, generated combination hypoxanthine xanthine oxidase (HX/XO), decreased sialic acid content surface, as indicated flow cytometric analysis involving acid-specific lectins, concomitant increase free was observed in supernatant. A assay showed that HX/XO treatment HL60 cells...
Nonalcoholic fatty liver disease (NAFLD) is one of the most prevalent chronic diseases, and results in development fibrosis. Oxidative stress thought to be underlying causes NAFLD. Copper/zinc superoxide dismutase (SOD1) a primary antioxidative enzyme that scavenges anion radicals. Although SOD1 knockout (KO) mice have been reported develop livers, it not known whether this lack leads Since accumulation collagen typically precedes fibrosis, we assessed balance between synthesis degradation...
Mammalian thioredoxin reductase (TR) is a flavoprotein catalysing reduction of oxidized in an NADPH-dependent manner, and contains selenocysteine (Sec) residue near the C-terminus. We observed that TR activity was decreased A549 cells by lowering fetal bovine serum content culture medium recovered addition selenium. To study role Sec activity, we have isolated full-length clone rat cDNA (3.3 kb) expressed it COS-1 transient-expression system. activities expressing were increased accordance...
We previously showed by in vitro experiments that the cysteine residue (Cys111) near dimer interface is critical for monomerization and resultant aggregate formation of mutant Cu, Zn-superoxide dismutase (SOD1) protein, which toxic to motor neurons familial amyotrophic lateral sclerosis (ALS). To verify importance Cys111 SOD1-associated ALS pathogenesis vivo, we analyzed disease phenotype SOD1 transgenic mice harboring H46R mutation alone (H46R mice) or H46R/C111S double mutations...
Accumulating evidence suggests that pathogenic TAR DNA-binding protein (TDP)-43 fragments contain a partial RNA-recognition motif domain 2 (RRM2) in amyotrophic lateral sclerosis (ALS)/frontotemporal lobar degeneration. However, the molecular basis for how this links to conformation and function of TDP-43 is unclear. Previous crystal analyses have documented RRM2-DNA complex dimerizes under acidic high salt conditions, mediated by intermolecular hydrogen bonds Glu246-Ile249 Asp247-Asp247....
Amyotrophic lateral sclerosis (ALS) involves the progressive degeneration of motor neurons in spinal cord and cortex. It has been shown that 15-20% patients with familial ALS (FALS) have defects Sod1 gene encodes Cu, Zn-superoxide dismutase (SOD). To elucidate pathological role mutated Zn-SODs FALS, susceptibility mutants to glycation was examined. Mutated (G37R, G93A, I113T) related FALS wild type were produced a baculovirus/insect cell expression system. Glycated nonglycated proteins...
Star anise is an important fragrance material that has a characteristic anise-like odor. Although the main component of star (E)-anethole, which accounts for over 90% constituents, odor (E)-anethole different from itself. Here, we examined aroma profile anise. GC-MS analysis extracts showed it contains many compounds with structures similar to (E)-anethole. Our results indicate key compound in anise, but structurally play role creating its We structure-odor relationship focusing on methoxy...