A5005970936- Lipid Membrane Structure and Behavior
- Immune Response and Inflammation
- Sphingolipid Metabolism and Signaling
- Glycosylation and Glycoproteins Research
- Cell Adhesion Molecules Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Protease and Inhibitor Mechanisms
- Caveolin-1 and cellular processes
- Platelet Disorders and Treatments
- Monoclonal and Polyclonal Antibodies Research
- Immune Cell Function and Interaction
- Antimicrobial Peptides and Activities
- Erythrocyte Function and Pathophysiology
- S100 Proteins and Annexins
- Phagocytosis and Immune Regulation
- Immunotherapy and Immune Responses
- Protein Kinase Regulation and GTPase Signaling
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Immune cells in cancer
- Chronic Lymphocytic Leukemia Research
- Blood Coagulation and Thrombosis Mechanisms
- Galectins and Cancer Biology
- Biochemical and Structural Characterization
- Cancer-related gene regulation
- Cell death mechanisms and regulation
Juntendo University
2014-2024
Juntendo University Urayasu Hospital
2015-2024
Juntendo University Hospital
2022
Gender Studies
2017
Fujita Health University
1999-2014
Fukuoka Higashi Medical Center
2014
Kyushu University
2014
In-Q-Tel
2012
RIKEN
1999-2004
Pacific Northwest Diabetes Research Institute
1998-2003
Summary The mast cell is one of the major effector cells in inflammatory reactions and can be found most tissues throughout body. During inflammation, an increase number local milieu occurs, such accumulation requires directed migration this population. As it has previously been reported that human cathelicidin‐derived antibacterial peptide, LL‐37, stimulates degranulation cells, we hypothesized LL‐37 could a chemotaxin. present study shows potent chemotactic factor for cells. response was...
Abstract Background Oxidized low-density lipoprotein (oxLDL) uptake by macrophages plays an important role in foam cell formation. It has been suggested the presence of heterogeneous subsets macrophage, such as M1 and M2, human atherosclerotic lesions. To evaluate which types contribute to atherogenesis, we performed cDNA microarray analysis determine oxLDL-induced transcriptional alterations each subset macrophages. Results Human monocyte-derived were polarized toward or M2 subset, followed...
Mouse melanoma B16 cells are characterized by the predominant presence of ganglioside GM3 and adhere to lactosylceramide- or Gg3-coated plates through interaction with lactosylceramide Gg3, whereby not only adhesion but also spreading enhancement cell motility occur (Kojima, N., Hakomori, S. (1991) J. Biol. Chem. 266, 17552–17558). We now report that process is based essentially on a glycosphingolipid-enriched microdomain (GEM) at surface, since >90% present in original found GEM, GEM...
Two membrane subfractions, one enriched in GM3 ganglioside and the other containing caveolin, were separated from low density detergent-insoluble fraction prepared by sucrose gradient centrifugation of postnuclear mouse melanoma B16 cells. The GM3-enriched subfraction, anti-GM3 monoclonal antibody DH2, contained sphingomyelin, cholesterol, c-Src, Rho A but not caveolin. In contrast, caveolin-containing anti-caveolin antibody, neither GM3, nor did contain glucosylceramide, Ras, a very small...
ABSTRACT After the first attachment of virus to cell surface through a primary receptor, efficient entry requires presence coreceptor. For adeno-associated type 2 (AAV2) infection, heparan sulfate proteoglycan is supposed as and αvβ5 integrin FGFR1 are reported act coreceptors. In this study, we were able demonstrate that hepatocyte growth factor c-Met, also coreceptor for AAV2 infection. AAV2-mediated transgene analyses revealed c-Met expression significantly up-regulated without increasing...
Mast cells are known to accumulate at the sites of inflammation in response chemoattractants generated local milieu. Since human β‐defensin‐2 (hBD‐2) is several epithelial tissues where mast present and because we have recently reported that this antibacterial peptide induces cell degranulation, thus hypothesized hBD‐2 could be a chemotaxin. Here report directly specifically migration with an optimal concentration 3 µg/ml. Checkerboard analysis showed was more chemotactic rather than...
There is a limited number of methods to examine transbilayer lipid distribution in biomembranes. We employed freeze-fracture replica-labelling immunoelectron microscopy combination with lipid-binding proteins and peptide both lateral various phospholipids mammalian cells. Our results indicate that are exclusively distributed either the outer or inner leaflet human red blood cell (RBC) membranes. In contrast, nucleated cells, such as skin fibroblasts neutrophils, sphingomyelin was leaflets...
Differentiation and neuritogenesis of mouse neuroblastoma Neuro2a cells are induced by exogenous ganglioside but not nerve growth factor because its receptor is absent in these cells. In view the emerging concept "glycosphingolipid-enriched domain" (GEM), we studied mechanism effect, focusing on structure function such a domain. GEM cells, separated as low density membrane fraction, contains essentially all glycosphingolipids sphingomyelin, together with five signal transducer molecules...
Laminin-1, an extracellular matrix molecule, promotes neurite outgrowth through the interaction of integrin and actin. Monosialoganglioside GM1 in lipid rafts associates with activates NGF receptor TrkA, enhances outgrowth. However, role laminin-1-induced was still unclear. Here, we describe that laminin-1 binds to a carbohydrate moiety specific conformation GM1, induces focal formation large clusters relocation TrkA membrane dorsal root ganglion (DRG) PC12 cells. We found laminin-1-mediated...
Mycobacterial-specific glycolipids determine pathogenesis after the mycobacteria are engulfed by human neutrophils.
Abstract A recombinant soluble form of human Fas ligand (sFasL) was tested for its chemotactic activity against and mouse polymorphonuclear neutrophils (PMN) by the Boyden chamber method. sFasL exhibited a potent both PMN HL-60 cells when differentiated into or monocytes. neutralizing anti-FasL mAb abolished activity, while control did not. Ligation either IgM- IgG-type anti-Fas also induced migration. derived from lpr mice that express few molecules not respond to sFasL. In contrast, those...
Abstract Polysaccharide β-1,3-D-glucans (β-glucans) are components of the cell wall various fungi and show immunomodulatory activities. β-Glucans have been reported to enhance neutrophil accumulation during pathogenic fungi-induced lung inflammation. Therefore, we examined whether β-glucans themselves possess chemotactic activities for human neutrophils. Among several kinds β-glucans, β-1,6-long glucosyl side-chain-branched β-glucan, isolated from Candida albicans [Candida soluble β-D-glucan...
Abstract The integrin CD11b/CD18 plays a central role in neutrophil phagocytosis. Although binds wide range of ligands, including C3bi and β-glucan, transmits outside-in signaling, the mechanism this signaling responsible for phagocytosis remains obscure. Here, we report that lactosylceramide (LacCer)-enriched lipid rafts are required CD11b/CD18-mediated nonopsonized zymosans (NOZs) by human neutrophils. Anti-CD11b anti-LacCer antibodies inhibited binding NOZs to neutrophils NOZs. During...
Macrophages are key immune system cells involved in inflammatory processes. Classically activated (M1) macrophages characterized by strong antimicrobicidal properties, whereas alternatively (M2) wound healing. Severe inflammation can induce postoperative complications during the perioperative period. Invasive surgical procedures polarization to M1 and associated complications. As management, it is an important strategy regulate functions of Although propofol has been found exhibit...
Cholesterol-rich membrane domains function in various events as diverse signal transduction and traffic. We studied the interaction of a fluorescein ester polyethylene glycol-derivatized cholesterol (fPEG-Chol) with cholesterol-rich membranes both cells model membranes. Unlike filipin other probes, this molecule could be applied an aqueous dispersion to samples. When added live cells, fPEG-Chol distributed exclusively outer plasma leaflet was enriched microdomains that dynamically clustered...
A protein analysis using a mass spectrometry indicated that there are serum proteins showing significant quantitative changes after the administration of infliximab. Among them, connective tissue growth factor (CTGF) seems to be related pathogenesis rheumatoid arthritis (RA). Therefore, this study was conducted investigate how CTGF is associated with disease progression RA.Serum samples were collected from RA patients in active or inactive stages, and before treatments production evaluated...
Lactosylceramide [LacCer; β-Gal-(1-4)-β-Glc-(1-1)-Cer] has been shown to contain very long fatty acids that specifically modulate neutrophil properties. The interactions between LacCer and proteins their role in cell signaling processes were assessed by synthesizing two molecular species of azide-photoactivable tritium-labeled having acyl chains different lengths. lengths the corresponded those a short/medium acid, comparable stearic lignoceric acids, respectively. These derivatives,...