A5076929039- Polysaccharides and Plant Cell Walls
- Fungal Biology and Applications
- Antifungal resistance and susceptibility
- Immune Response and Inflammation
- Microbial Metabolites in Food Biotechnology
- Glycosylation and Glycoproteins Research
- Seaweed-derived Bioactive Compounds
- Enzyme Production and Characterization
- Antimicrobial Peptides and Activities
- Fungal Infections and Studies
- Herbal Medicine Research Studies
- NF-κB Signaling Pathways
- Kawasaki Disease and Coronary Complications
- Streptococcal Infections and Treatments
- Phytochemistry and Bioactivity Studies
- Carbohydrate Chemistry and Synthesis
- Galectins and Cancer Biology
- Food composition and properties
- Polysaccharides Composition and Applications
- Pharmacological Effects of Natural Compounds
- Immune Cell Function and Interaction
- Studies on Chitinases and Chitosanases
- Cell Adhesion Molecules Research
- Pediatric health and respiratory diseases
- Nitric Oxide and Endothelin Effects
Tokyo University of Pharmacy and Life Sciences
2016-2025
Meteorological Research Institute
2024
Japan Meteorological Agency
2024
Pfizer (United Kingdom)
2021
Seascape Consultants (United Kingdom)
2020
Caterpillar (United States)
2020
Kyiv City Clinical Oncology Center
2019
Ecologie Microbienne Lyon
2013
Charité - Universitätsmedizin Berlin
2012
Life Science Institute
2012
Toll-like receptors (TLRs) are innate recognition molecules for microbial products, but their direct interactions with corresponding ligands remain unclarified. LPS, a membrane constituent of gram-negative bacteria, is the best-studied TLR ligand and recognized by TLR4 MD-2, molecule associated extracellular domain TLR4. Although TLR4-MD-2 recognizes little known about physical interaction between LPS TLR4-MD-2. Here, we demonstrate cell surface LPS–TLR4-MD-2 complexes. CD14 greatly enhances...
To rapidly respond to invading microorganisms, humans call on their innate immune system. This occurs by microbe-detecting receptors, such as CD14, that activate cells eliminate the pathogens. Here, we link lipopolysaccharide receptor CD14 with Alzheimer's disease, a severe neurodegenerative disease resulting in dementia. We demonstrate this key immunity interacts fibrils of Alzheimer amyloid peptide. Neutralization antibodies against and genetic deficiency for significantly reduced peptide...
Abstract Mammalian myeloid and epithelial cells express several kinds of antibacterial peptides (α-/β-defensins cathelicidins) that contribute to the innate host defense by killing invading micro-organisms. In this study we evaluated LPS-neutralizing activities cathelicidin human CAP18 (cationic proteins 18 kDa) guinea pig CAP11 using CD14+ murine macrophage cell line RAW264.7 endotoxin shock model. Flow cytometric analysis revealed inhibited binding FITC-conjugated LPS cells. Likewise,...
Dectin 1 is a mammalian cell surface receptor for (1-->3)-beta-d-glucans. Since (1-->3)-beta-d-glucans are commonly present on fungal walls, it has been suggested that dectin important recognizing invasion. In this study we tried to deduce the amino acid residues in responsible beta-glucan recognition. HEK293 cells transfected with mouse cDNA could bind gel-forming (1-->3)-beta-d-glucan, schizophyllan (SPG). The binding of SPG transfectant was inhibited by pretreatment other beta-glucans...
Agaricus blazei is a medically important mushroom widely eaten and prescribed in Japan. Polysaccharide fractions were prepared from cultured A. by repeated extraction with hot water (AgHWE), cold NaOH (AgCA), then (AgHA). By chemical, enzymic, NMR analyses, the primary structures of AgHWE, AgCA, AgHA mainly composed 1,6-beta-glucan. Among these fractions, extracts showed antitumor activity against solid form Sarcoma 180 ICR mice. To demonstrate active component several chemical enzymic...
The ability of grifolan (GRN), a purified fungal (1-->3)-beta-D-glucan, to induce various cytokines from macrophages was examined in vitro. Interleukin-6 (IL-6) activity supernatants the culture macrophage cell line, RAW264.7 dependent on increasing doses GRN. level IL-6 induced with 500 micrograms/ml GRN comparable that lipopolysaccharide (LPS) 10 micrograms/ml. Enhancement mRNA by treatment detected reverse transcriptase-polymerase chain reaction (RT-PCR). effect production also observed...
Dectin-1 (gene Clec7a), a receptor for β-glucans, plays important roles in the host defense against fungi and immune homeostasis of intestine. Although this molecule is also suggested to be involved regulation tumorigenesis, role intestinal tumor development remains elucidated. In study, we find that azoxymethane-dextran-sodium-sulfate-induced ApcMin-induced tumorigenesis are suppressed Clec7a-/- mice independently from commensal microbiota. preferentially expressed on myeloid-derived...
Mammalian myeloid and epithelial cells express various peptide antibiotics (such as defensins cathelicidins) that contribute to the innate host defense against invading microorganisms. Among these peptides, human cathelicidin CAP18/LL-37 (L(1) S(37)) possesses not only potent antibacterial activity gram-positive gram-negative bacteria but also ability bind lipopolysaccharide (LPS) neutralize its biological activities. In this study, develop derivatives with improved LPS-neutralizing...
In a previous study, we reported that one of the gel-forming (1-->3)-beta-D-glucans, grifolan (from Grifola frondosa, GRN), stimulated cytokine production from macrophages in vitro. However, several other such as sonifilan (SPG) and SSG, did not induce macrophages. The ultrastructure especially triple- single-helix, does affect cytokine-inducing activity. action on tumor necrosis factor alpha (TNF alpha) release was correlated with molecular weight GRN, since highest fraction Mr > or =...
Abstract The mechanism of host cell recognition Cryptococcus neoformans, an opportunistic fungal pathogen in immunocompromised patients, remains poorly understood. In the present study, we asked whether DNA this yeast activates mouse bone marrow-derived myeloid dendritic cells (BM-DCs). BM-DCs released IL-12p40 and expressed CD40 upon stimulation with cryptococcal DNA, response was abolished by treatment DNase, but not RNase. production expression were attenuated chloroquine, bafilomycin A,...
The innate immune system of humans recognizes the human pathogenic fungus Candida albicans via sugar polymers present in cell wall, such as mannan and beta-glucan. Here, we examined whether nucleic acids from C. activate dendritic cells. DNA induced interleukin-12p40 (IL-12p40) production CD40 expression by murine bone marrow-derived myeloid cells (BM-DCs) a dose-dependent manner. BM-DCs that lacked Toll-like receptor 4 (TLR4), TLR2, dectin-1, which are pattern recognition receptors for...
Abstract Polysaccharide β-1,3-D-glucans (β-glucans) are components of the cell wall various fungi and show immunomodulatory activities. β-Glucans have been reported to enhance neutrophil accumulation during pathogenic fungi-induced lung inflammation. Therefore, we examined whether β-glucans themselves possess chemotactic activities for human neutrophils. Among several kinds β-glucans, β-1,6-long glucosyl side-chain-branched β-glucan, isolated from Candida albicans [Candida soluble β-D-glucan...
Abstract Dectin‐1 is known as a sole receptor for β‐glucan, major cell wall component of fungal microorganisms. In the current study, we examined role this molecule in host defense to Cryptococcus neoformans , an opportunistic pathogen AIDS patients. There was no significant difference clinical course and cytokine production between dectin‐1 gene‐deficient control mice. These results indicate that not likely essential development protective responses C. .
The immunopharmacological activities of a fungal (1-->3)-beta-D-glucan, OL-2, isolated from "Leiwan" Omphalia lapidescens were examined. Intraperitoneal (i.p.) administration OL-2 to ICR mice induced significant number peritoneal exudate cells (PEC) and white blood over the period few days. Spleen cell numbers also increased by i.p. at about week. These changes reverted normal level within month. Responses spleen bone marrow (BM) colony stimulating factors (CSF) augmented assessed...
Changes of biological activities manifested by (1----6)-branched (1----3)-beta-D-glucans various molecular weights obtained heat treatment the corresponding intact beta-glucan at 150 degrees C (HD-LE) were examined. The assessed in this study as follows: an antitumor activity, activation alternative complement pathway, glucose consumption macrophages, macrophage-mediated lysosomal enzyme activity culture supernatant and cell lysate, interleukin-1 (IL-1) adjuvant activity. HD-LE could be...
The present study was designed to elucidate the role of Toll-like receptor (TLR) 2 and TLR4 in host response Cryptococcus neoformans. Both TLR2 knockout (KO) TLR4KO mice produced interleukin-1beta (IL-1beta), IL-6, IL-12p40 tumor necrosis factor-alpha (TNF-alpha) sera cleared this fungal pathogen from infected lungs at a comparable level control littermate (LM) mice. Synthesis these cytokines not significantly different KO LM mice, although IL-1beta, IL-6 tended be lower TLR2KO, but TLR4KO,...
Chemical characterization of the extracts, and purification structural an antitumor glucan obtained from liquid-cultured mycelium Grifola frondosa are described in this paper. The (13 g dry weight/1 culture) was extracted successively with hot water (LMHW, 14.9 g/100 mycelium), cold alkali (LMCA, 6.3 (LMHA, 4.5 mycelium). Each extract dialyzed then polysaccharide fraction precipitated ethanol. Another portion incubated a buffer composed glucose (5.0%) citric acid, pH 4.5, for 3 d supernatant...
Cordyceps militaris, an entomopathogenic fungus traditionally used in East Asian medicine, contains 1,3-β-glucans with well-known immunomodulatory properties. Our previous research has demonstrated that both fruit body powder and hot water extract of C. militaris can activate bone marrow-derived dendritic cells through dectin-1 signaling. However, the immunological effects on Fms-like tyrosine kinase 3 ligand-induced (fDCs), which closely resemble steady-state conventional vivo, remain...