Abstract Background Periodontitis is the most common oral disease in dogs, and its progression severity are influenced by risk factors, such as age body size. Recent studies have assessed canine microbiota relation to different stages of periodontitis niches within cavity. However, knowledge bacterial composition at ages sizes, especially puppies, limited. This study aimed characterize healthy gingiva small breed puppies using next-generation sequencing. Additionally, we impact dental care...

To investigate the interrelationships among concerns regarding dental visits, status of regular and periodontal health during coronavirus disease 2019 (COVID-19) pandemic.Continuous oral care visits are important for maintaining health. Due to possibility contracting COVID-19, individuals have been reluctant visit medical institutions. It is unclear how Japanese population has affected by interruption COVID-19 pandemic attendance at visits.This study included 199 office workers in one...

Background/Objectives: Previously, we reported that high molecular weight hyaluronic acid (HMW-HA) exerts chondroprotective effects by enhancing dual specificity protein phosphatase 10/mitogen-activated kinase (MAPK) 5 (DUSP10/MKP5) expression and suppressing inflammatory cytokine-induced matrix metalloproteinase-13 (MMP13) in a human immortalized chondrocyte line (C28/I2 cells) via inhibition of MAPKs. The aim this study was to elucidate the mechanisms underlying enhancement DUSP10/MKP5...

Introduction Recent studies have elucidated a potential correlation between oral carcinogenesis and the microbiome. However, few reports exist on interaction Streptococcus spp., most common microflora bacterium, cancer. In this study, we aimed to elucidate effects of spp. squamous cell carcinoma (OSCC) cells in vitro . Methods HSC-3 (tongue carcinoma) Ca9-22 (gingival were used as models OSCC cells, their responses examined after adding major species— S. mitis , sanguinis anginosus...

Abstract Aim To evaluate the association between sleep duration and severe periodontitis in Japanese workers. Materials methods This cross‐sectional study included 1130 workers (mean age 43.0 years) who underwent full‐mouth periodontal examinations health check‐ups completed a self‐administered questionnaire that questions on duration. Logistic regression restricted cubic spline model were used to analyse data. Results Severe was identified 6.3% of population. Those with <5, 5–5.9, 6–6.9,...

Chondrogenesis is strictly regulated by several factors, including cytokines, hormones, and extracellular matrix proteins. Mouse teratocarcinoma-derived lineage cells, differentiate into chondrocytes in the presence of insulin. Although ascorbic acid promotes chondrogenic differentiation, detailed regulative mechanisms underlying its role chondrogenesis remain unclear. Therefore, this study, we evaluated effects on insulin-induced differentiation ATDC5 cells intracellular signaling. The...

Abstract We aimed to assess the validity of self-report questionnaire for periodontitis in a Japanese population. A 9-item questionnaire, developed by translating English-version questions that were used detect periodontitis, was validated against full-mouth clinically-assessed 949 adults (average age = 43.2 years). Multivariable logistic regression modeling calculate area under receiver operating characteristic curve (AUC), wherein case definition Centers Disease Control and...

Orthodontic treatment requires the regulation of bone remodeling in both compression and tension sides. Transforming growth factor-β1 (TGF-β1) is an important coupling factor for remodeling. However, mechanism underlying TGF-β1-mediated osteoclast-supporting activity osteoblasts stromal cells remain unclear. The current study investigated effect TGF-β1 on receptor activator nuclear kappa-B ligand (RANKL) expression induced by 1α,25(OH)2D3 (D3) dexamethasone (Dex). downregulated RANKL D3 Dex...

ABSTRACT Osteoclasts derived from hematopoietic stem cells express immunoreceptors on their cell surface. Previously, we showed that the β‐glucan curdlan suppressed osteoclastogenesis via binding to dectin‐1, a pattern recognition receptor. Curdlan negatively regulates osteoclast differentiation and bone resorption capacity by suppressing expression of nuclear factor activated T 1 (NFATc1), master for differentiation, in dectin‐1‐dependent manner; however, mechanism involved this process has...

This study aimed to explore whether the Trypsin-Like Peptidase Activity Assay Kit (TLP-AA-Kit), which measures activity of N-benzoyl-dl-arginine peptidase (trypsin-like peptidase), can be used as a reliable tool for periodontitis detection in population-based surveillance. In total, 105 individuals underwent full-mouth periodontal examination and provided tongue swabs specimens further analyses. The results TLP-AA-Kit were scored between 1 5; higher scores indicated trypsin concentrations....

Immunoreceptors expressed on osteoclast precursor cells modify differentiation and bone resorption activity. Dectin-1 is a lectin receptor of β-glucan specifically in cells. In this study, we evaluated the bioactivity activator nuclear factor-kappa B ligand (RANKL)-induced osteoclastogenesis observed that glucan from baker's yeast inhibited process mouse marrow dectin-1-overexpressing RAW264.7 (d-RAW) conjunction, RANKL-induced factor activated T cell c1 expression was suppressed,...

In recent years, magnesium hydroxide has been widely studied due to its bioactivity and biocompatibility. The bactericidal effects of nanoparticles on oral bacteria have also reported. Therefore, in this study, we investigated the biological inflammatory responses induced by periodontopathic bacteria. Macrophage-like cells, namely J774.1 were treated with LPS derived from Aggregatibacter actinomycetemcomitans two different sizes (NM80/NM300) evaluate their response. Statistical analysis was...

The differentiation and function of osteocytes are controlled by surrounding cells mechanical stress; however, the detailed mechanisms unknown. Recent findings suggest that IL-33 is highly expressed in periodontal tissues orthodontic tooth movement. present study aimed to elucidate effect on expression regulatory factors for bone remodeling their molecular osteocyte-like cell line MLO-Y4. MLO-Y4 were treated with IL-33, activation intracellular signaling molecules transcriptional was...

Objectives N-benzoyl-DL-arginine peptidase (trypsin-like peptidase) is specifically produced by certain strains of periodontitis-associated bacteria. We aimed to examine the effectiveness an objectively quantified trypsin-like activity assay (TLP-AA) for detecting severe periodontitis. Methods The study population included 347 adults (108 men and 239 women; average age, 43.3 years) who underwent a full-mouth periodontal examination. Specimens TLP-AA were obtained using tongue swabs. Using...

Cancer stem cells (CSCs) are considered to be responsible for recurrence, metastasis, and resistance treatment in many types of cancers; therefore, new strategies targeting CSCs attracting attention. In this study, we fabricated a polyethylene glycol-tagged microwell device that enabled spheroid formation from human oral squamous carcinoma cells. HSC-3 Ca9-22 cultured the aggregated generated single per well within 24–48 h. The circular shape smooth surface spheroids were maintained up five...

Abstract Aim To evaluate the association between trypsin‐like protease (TLP) activity in oral cavity as an indicator of periodontal health status and kidney function Japanese workers. Materials Methods This cross‐sectional study included 1117 workers (mean age = 43.8 years). Tongue‐swab TLP was quantified a * value (the redness intensity matrix disc assessment kit; larger indicates more intense enzymatic samples poorer status). Kidney assessed using estimated glomerular filtration rate...